Integration of peripheral and central activity patterns in the devoping visual cortex [Elektronische Ressource] / Friederike Siegel. Betreuer: Tobias Bonhoeffer

Friederike Siegel

Sujets

• Gesundheit

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2010
• Nombre de lectures: 36
• Langue: Deutsch
• Poids de l'ouvrage: 4 Mo

Extrait

Integration of peripheral and
central activity patterns in the
developing visual cortex Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften der Fakultät für Biologie der Ludwig-Maximilians-Universität München Vorgelegt von Friederike Siegel München, 20. September 2010














Erstgutachter: Prof. Dr. Tobias Bonhoeffer Zweitgutachter: Prof. Dr. Rainer Uhl Tag der mündlichen Prüfung: 08. November 2010




















Ehrenwörtliche Versicherung: Ich versichere hiermit ehrenwörtlich, dass ich die Dissertation mit dem Titel “Integration of peripheral and central activity patterns in the developing visual cortex“ selbständig und ohne unerlaubte Hilfe angefertigt habe. Ich habe mich dabei keiner anderen als der von mir ausdrücklich bezeichneten Hilfen und Quellen bedient.
Erklärung: Hiermit erkläre ich, dass ich mich nicht anderweitig einer Doktorprüfung ohne Erfolg unterzogen habe. Die Dissertation wurde in ihrer jetzigen oder ähnlichen Form bei keiner anderen Hochschule eingereicht und hat noch keinen sonstigen Prüfungszwecken gedient. München, den 20. September 2010 Friederike Siegel

Die vorliegende Arbeit wurde zwischen Juli 2006 und September 2010 unter Anleitung von Dr. Christian Lohmann am Max-Planck-Institut für Neurobiologie in Martinsried sowie am Netherlands Institute for Neuroscience in Amsterdam, Niederlande durchgeführt.


Table of contents


List of Figures.................................................................................................................................................. 5
List of Tables ................................................................................................................................................... 6
Abbreviations ................................................................................................................................................. 7
1 Abstract ....................................................................................................................................................... 9
2 Introduction .......................................................................................................................................... 10 2.1 Nervous system development: The concerted interplay of activity-dependent mechanisms and molecular guidance cues ........................................................... 11 2.2 Spontaneous activity patterns guide the formation of neuronal networks ............. 13 2.3 Visual system development ......................................................................................................... 16 2.3.1 The mouse visual system...................................................................................................... 17 2.3.2 Molecular cues and visual system development ........................................................ 19 2.3.3 Activity-dependent mechanisms and visual system development ..................... 22 2.3.4 Visually evoked activity and critical period.................................................................. 29 2.4 Objective of the study ..................................................................................................................... 31
3 Material and methods ...................................................................................................................... 33 3.1 Material ................................................................................................................................................ 33 3.1.1 Chemicals .................................................................................................................................... 33 3.1.2 Buffers and solutions ............................................................................................................. 34 3.1.3 Pharmacological agents ........................................................................................................ 35 3.1.4 Equipment .................................................................................................................................. 35
3.1.5 Custom-built two-photon microscope (based on the MOM two-photon microscope, Sutter Instruments) ................................................................................................. 37 3.1.6 Software ....................................................................................................................................... 37 3.2 In vitro study to investigate the effects of anesthesia on spontaneous network activity in the developing cortex..................................................................................... 38 3.3 Surgery of neonatal mice to expose the visual cortex ....................................................... 39 3.4 Multi-cell bolus loading in the cortex of neonatal mice.................................................... 41 3.5 In vivo imaging of spontaneous calcium dynamics in the visual cortex of the neonatal mouse ........................................................................................................................................ 43 3.6 Manipulations of spontaneous activity patterns in the retina of developing mice ............................................................................................................................................................... 45 3.7 Analysis of spontaneous network activity in the developing cortex in vivo ........... 46
4 Results....................................................................................................................................................... 49 4.1 Network activity of organotypic cortical slice cultures is not affected by the anesthetic isoflurane .............................................................................................................................. 50 4.2 The developing visual cortex generates distinct patterns of spontaneous network activity in vivo ........................................................................................................................ 52 4.3 Binocular enucleation reduces network activity in the developing visual cortex ............................................................................................................................................................ 60 4.4 Binocular NKH477 injections increase the frequency of cortical network events ............................................................................................................................................................ 65 4.5 Distinct mechanisms of spontaneous activity drive cortical network events......... 69 4.6 Binocular epibatidine injections have ambiguous effects on cortical network activity ....................................................................................................................................... 72 4.7 Comparison of the effects of different retinal manipulations on cortical network activity ....................................................................................................................................... 75