Nanobiotechnology: tools for proteomics [Elektronische Ressource] : molecular organization and manipulation of proteins and protein complexes in nanodimensions / von Ali Tinazli

Mindlabs

Sujets

• Biologie

Informations

• Publié par: goethe_universitat_frankfurt_am_main
• Publié le: 01 janvier 2007
• Nombre de lectures: 112
• Langue: English
• Poids de l'ouvrage: 15 Mo

Extrait

Nanobiotechnology: Tools for Proteomics
–
Molecular Organization and Manipulation of
Proteins and Protein Complexes
in Nanodimensions










Dissertation
zur Erlangung des Doktorgrades
der Naturwissenschaften

vorgelegt beim Fachbereich
Chemische und Pharmazeutische Wissenschaften (FB 14)
der Johann Wolfgang Goethe-Universität am Main
von
Ali Tinazli
aus Bad Homburg v. d. H.
2
















Vom Fachbereich Chemische und Pharmazeutische Wissenschaften (FB 14)
der Johann Wolfgang Goethe-Universität als Dissertation angenommen.



Dekan: Prof. Dr. H. Schwalbe
Gutachter: Prof. Dr. R. Tampé Prüfungskommission: Prof. Dr. R. Tampé
Prof. Dr. E. Bamberg Prof. Dr. E. Bamberg
Prof. Dr. H.-J. Butt B. Kolbesen
Prof. Dr. B. Ludwig
Datum des Druckes: 14. Februar 2007
Datum der Disputation: 02. Juli 2007
Note: sehr gut „summa cum laude“ 3
Publications & Patents
1. R. Gamsjäger, B. Wimmer, H. Kahr, A. Tinazli, S. Pi ćuri ć, S. Lata, R. Tampé, Y. Maulet, H. J.
Gruber, P. Hinterdorfer, and C. Romanin, Oriented binding of the His -tagged carboxyl-tail of the 6
2+L-type Ca channel 1-subunit to a new NTA-functionalized self-assembled monolayer.
2004, Langmuir, 20, 5885-5890.
2. S. Hutschenreiter, A. Tinazli, K. Model, and R. Tampé, Two-substrate association with the
20S proteasome at single-molecule level. 2004, EMBO J., 23, 2488-2497.
3. A. Tinazli & R. Tampé, Small is beautiful – Bioforschung in der Nanowelt.
2004, Forschung Frankfurt, 3-4, 10-14.
4. A. Tinazli, J. Tang, R. Valiokas, S. Pi ćuri ć, S. Lata, J. Piehler, B. Liedberg, and R. Tampé, High-
affinity chelator thiols for switchable and oriented immobilization of histidine-tagged proteins:
a generic platform for protein chip technologies. 2005, Chem. Eur. J., 11, 5249-5259.
5. G. Klenkar, R. Valiokas, I. Lundström, A. Tinazli, R. Tampé, J. Piehler, and B. Liedberg, Piezo
dispensed microarray of multivalent chelating thiols for dissecting complex protein-protein
interactions. 2006, Anal. Chem.,78, 3643-3650.
6. K. Schulze, A. Mulder, A. Tinazli, and R. Tampé, Activity control of the 20S proteasome complex
by synthetic gatekeepers. 2006, Angew. Chem. Int. Ed. Engl., 45, 5702-5205.
7. R. Valiokas, G. Klenkar, A. Tinazli, R. Tampé, B. Liedberg, and J. Piehler, Differential protein
assembly on micropatterned surfaces with tailored molecular and surface multivalency.
2006, ChemBioChem, 7, 1325-1329.
8. F. Kienberger, H. Artelsmaier, A. Tinazli, C. Rankl, R. Tampé, H. Gruber, P. Hinterdorfer, D. Blaas,
AFM-based nanochip for the detection and characterization of viruses.
2006, patent No. A961/2006.
9. C. D. Hahn, C. Leitner, T. Weinbrenner, R. Schlapak, A. Tinazli, R. Tampé, B. Lackner, C. Steindl,
P. Hinterdorfer, H. J. Gruber, and M. Hölzl, Self-assembled monolayers with latent aldehydes for
protein immobilization. 2007, Bioconjug. Chem., 18, 247-253.
10. C. D. Hahn, A. Tinazli, M. Hölzl, C. Leitner, P. J. Winklehner, F. Frederix, B. Lackner, N. Müller,
C. Klampfl, F. Kienberger, P. Hinterdorfer, R. Tampé, and H. J. Gruber, Pragmatic studies on
protein-resistant gold surfaces. 2007, Chemical Monthly, 01/2007 online first article.
11. C.Q. Scheckhuber, N. Erjavec, A. Tinazli, T. Nyström, and H. D. Osiewacz, Reducing
mitochondrial fission by deleting Dnm1 increases the health span in two fungal ageing models.
2007, Nature Cell Biol., 9, 99-105.
12. A. Tinazli, J. Piehler, M. Beuttler, R. Guckenberger, and R. Tampé, Native protein
nanolithography that can write, read and erase. 2007, Nature Nanotech., 2, 220-225. 4
13. M. Hölzl, A. Tinazli, C. Leitner, C. D. Hahn, B. Lackner, R. Tampé, and H. J. Gruber Protein-
resistant SAMs on gold with latent aldehyde functions. 2007, Langmuir, 23, 5571-5577.
14. K. Thelen, T. Wolfram, B. Maier, S. Jährling, A. Tinazli, J. Piehler, J. P. Spatz, and G. E. Pollerberg
Cell adhesion molecule DM-GRASP presented in different nanopatterns to neurons regulates
attachment and neurite growth. 2007, submitted.
15. F. Kienberger, H. Artelsmair, A. Tinazli, R. Schlapak, G. Kada, J. Wruss, C. Rankl, S. Howorka,
R. Tampé, D. Blaas, H. Gruber, and P. Hinterdorfer, Nanochip for the detection and
characterization of single virus particles. 2007, submitted.
16. A. Turchanin, A. Tinazli, M. El-Desawy, R. Tampé, and A. Gölzhäuser, E-beam nanolithography
meets molecular self-assembly and chemical biology – a new route for protein chip fabrication.
2007, in preparation.
17. T. Hoang, A. Tinazli, R. Tampé, and M. Karas, Protein chips for purification and analysis of
phosphopeptides by mass spectrometry. 2007, in preparation.
18. A. Tinazli, K. Buchholz, A. Kleefen, R. Tampé, and M. Tornow, SOI nanopored cavities for lipid
membrane investigations. 2007, in preparation.

Lectures & Posters
09/2006 Department of Medicine, J. W. Goethe University, Frankfurt am Main, Germany (oral)
08/2006 Faculty of Chemistry, Heinrich Heine University Düsseldorf, Germany (oral)
07/2006 Faculty of Chemistry, Philipps University Marburg, Germany (oral)
06/2006 Fujirebio Inc., Tokyo, Japan (oral)
03/2006 Interdisciplinary Nanoscience Center (iNano), University of Aarhus, Denmark (oral)
02/2006 VIII. Annual Linz Winter Workshop, Linz, Austria (oral)
02/2006 DECHEMA status seminar chip technologies, Frankfurt am Main, Germany (poster)
11/2005 2nd Nanotechnology forum Hesse, Hanau, Germany (poster)
11/2005 Invited speaker at the faculty celebration of the J. W. Goethe University, Frankfurt am Main,
Germany (oral)
09/2005 Chemical Nanotechnology Talks VI, Frankfurt am Main, Germany (poster)
09/2005 International Summer School Hirschegg 2005, Austria (oral)
07/2005 Bio-Image Summer School “Visualization, Manipulation and Modeling of Single
Biomolecules”, Paris, France (poster)
05/2005 3rd International Workshop to Develop a Global Nanotechnology Network, Saarbrücken,
Germany (poster) 5
05/2005 Bielefeld Institute for BioPhysics and Nanoscience, University of Bielefeld, Germany (oral)
04/2005 Faculty of Biology, Ruhr University Bochum, Germany (oral)
04/2005 NanoBionics III – from Molecules to Applications, Marburg, Germany (poster);
stPresentation was awarded with the 1 Prize.
02/2005 VII. Annual Linz Winter Workshop, Austria (oral)
11/2004 1st Nanotechnology forum Hesse, Hanau, Germany (poster)
09/2004 International Summer School Hirschegg 2004, Austria (oral)
09/2004 MESA+ Institute for Nanotechnology, University of Twente, The Netherlands (oral)
09/2004 Faculty of Chemistry, Philipps University Marburg, Germany (oral)
02/2004 Applied Biosystems – Applera Deutschland GmbH, Darmstadt, Germany (oral)
10/2003 1st BMBF-Symposium Nanobiotechnology, Hannover, Germany (poster)
09/2003 Scanning Probe Microscopy and Organic Materials XII, Mainz, Germany (oral)
09/2003 International Summer School Hirschegg 2003, Austria (oral)
09/2003 Faculty of Chemistry, Philipps University Marburg, Germany (oral)
02/2003 DECHEMA status seminar chip technologies, Frankfurt am Main, Germany
02/2003 V. Annual Linz Winter Workshop, Austria (poster)
12/2002 Max-Planck-Institute of Biochemistry, Martinsried, Germany (oral)
09/2002 3rd International Symposium on Physics, Chemistry and Biology with Single Molecules,
Tutzing, Germany
09/2002 International Summer School Hirschegg 2002, Austria (oral)

Grants
The results of this Ph.D. thesis contributed significantly to successfully approved research grants
from the “Bundesministerium für Bildung und Forschung” (BMBF)”:

• Einschritt-Fabrikation von Biochip Nanotemplaten: Molekulare Gasphasenabscheidung zur
Funktionalisierung nanostrukturierter Oberflächen (BMBF)
• MultiplexLAB on NanoChip: Multiplexes elektrisches, mechanisches und optisches
Auslesen von biologischen Membranprozessen an Silizium-Nanoporenchips (BMBF) 6

7
Acknowledgment

This work was enabled by a fruitful atmosphere at the interface of biology, chemistry, and physics.
First of all, I want to thank my “Doktorvater” Prof. Dr. Robert Tampé for his continuous challenging,
motivation, and teaching of science. He always supported me in the best way and provided me a stimulating
scientific environment which gave me the freedom to operate as independent as possible. I also appreciated
his special support for one of my favourite music style: Robert always encouraged me to play-back Black
Metal (e.g. Khold) at Christmas parties and other occasions of public joy.
Special thanks appertain to Prof. Dr. Jacob Piehler. He was always open for discussing
experimental or conceptual issues. Furthermore, he had this kind of humor which enabled an autocatalytic
feedback loop of laughter at different occasions (e.g. “RNA squirrels” in Marburg).
Another very important fundament for this Ph.D. was the ability to evaporate gold. Herefore I am
very grateful to Prof. Dr. Ernst Bamberg a