Structural studies on kinesin-1 motor domain and light chain from Rattus norvegicus and Neurospora crassa [Elektronische Ressource] / presented by Spyridon Mylonas

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Structural Studies on Kinesin-1 Motor Domain and Light Chain from Rattus norvegicus and Neurospora crassa Thesis Submitted to the University of Hamburg in Partial Fulfilment of the Requirements for the Degree of Ph.D. Presented by Spyridon Mylonas from Greece Hamburg, 2005 Table of contents i 1. Introduction 1 1.1 Microtubule-associated motor: the kinesin superfamily 1 1.2 Conventional kinesin (kinesin-1) 4 1.2.1 Biological function of kinesin-1 6 1.2.2 Structure of the motor domain of kinesin-1 7 1.3 Kinesin light chains 11 1.3.1 The tetratricopeptide repeat region of KLC 13 1.4 Aim of this study 16 2 Materials and methods 17 2.1 17 2.1.1 Chemicals 2.1.2 Enzymes 2.1.3 Bacterial strains 18 2.1.4 Cloning Vectors 19 2.1.5 Expression 2.1.6 Media 20 2.1.7 Crystallization 20 2.1.8 Equipment and accessories 21 2.2 Molecular biology and microbiological methods 22 2.2.1 Culture and storage of E.coli 22 2.2.2 Transformation of E.coli strains Transformation by heat-shock 22 Transformation electroporation 23 2.2.3 Isolation of plasmid DNA 23 2.2.4 Determination of DNA concentration and purity 23 2.2.5 Ligation reaction 24 2.2.

Sujets

• Biologie

Informations

• Publié par: universitat_hamburg
• Publié le: 01 janvier 2005
• Nombre de lectures: 26
• Langue: English
• Poids de l'ouvrage: 2 Mo

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