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Pga e 1fo1 (2apegum nr bet nor foaticnoitrup esops)
Research Open Access Two discrete events, human T-cell leukemia virus type I Tax oncoprotein expression and a separa te stress stimulus, are required for induction of apoptosis in T-cells Takefumi Kasai and Kuan-Teh Jeang*
Address: Laboratory of Molecular Microb iology, NIAID, National Institutes of Health, Bethesda, Maryland 20892-0460, USA Email: Takefumi Kasai - tkasai@m.kufm.kagosh ima-u.ac.jp; Kuan-Teh Jeang* - kj7e@nih.gov * Corresponding author
Background esses are dysregulated by Tax in parallel (reviewed in Human T-lymphotropic virus type I (HTLV-I) causes adult [1,13,14]. This is likely explained by the fact that Tax can T-cell leukemia (ATL; reviewed in [1-3]). ATL develops in activate NF-κ B, SRF , and CREB/ATF-responsive genes and -a minority of HTLV-I infected individuals with a long can markedly accelerate cell cycle progression [15]; latent period. This pathological course suggests a multi- reviewed in [16]. stage process of immortalization and transformation of T-lymphocyte. HTLV-I encodes a 40 kDa phosphoprotein, The ability to transform and the ability to engender apop-Tax. Tax immortalizes T- lymphocytes [4-6] and trans- tosis seem to be contradictory functions. Interestingly, forms rat fibroblasts [7,8]. Tax is also a transcriptional both abilities have been widely reported in the literature activator of the HTLV-I LTR [9-11]; reviewed in [12]. for the HTLV-I Tax protein. Tax has been shown to inhibit While the exact events leading to transformation are apoptosis [4,5,17-22]. On the other hand, Tax has also incompletely understood, several important cellular proc- been shown to induce apoptosis [23-32]. Indeed Kao et al.
Bio Med  Central
Abstract Background: It is poorly understood why many transf orming proteins reportedly enhance both cell growth (transformation) and ce ll death (apoptosis). At first glance, the ability to transform and the ability to engender apoptosis seem to be cont radictory. Interestingly, both abilities have been widely reported in the literatu re for the HTLV-I Tax protein. Results: To reconcile these apparently divergent find ings, we sought to understand how Tax might cause apoptosis in a Jurkat T-cell line, JPX-9. Tax expression can be induced equally by either cadmium (Cd) or zinc (Zn) in JP X-9 cells. Surprisingly, when induced by Zn, but not when induced by Cd, Tax-expression produced significant apoptosis. Under our experimental conditions, Zn but not Cd, induced SAPK (stress activated protein ki nase)/JNK (Jun kinase) activation in cells. We further showed that transient over-expression of Tax-alone or Jun-alone did not induce cell death. On the other hand, co-expressi on of Tax plus Jun did effe ctively result in apoptosis. Conclusion: We propose that Tax-expression alone in a T-cell background insufficiently accounts for apoptosis. On the other hand, Ta x plus activation of a stress kina se can induce cell death. Thus, HTLV-I infection/transformation of cells requires two discrete even ts (i.e. oncoprotein expression and stress) to produce apoptosis.
Retrovirology
Published: 06 May 2004 Received: 23 April 2004 Retrovirology 2004, 1 :7 Accepted: 06 May 2004 This article is available from: http ://www.retrovirology.com/content/1/1/7 © 2004 Kasai and Jeang; licensee BioMed Cent ral Ltd. This is an Open Access article: verbatim copying and redistribution of thi s article are permitted in all media for any purpose, provided this notice is preserved along with the article s original URL. '
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