Mercury resistance (mer) operons in enterobacteria abstract

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Publié le : jeudi 21 juillet 2011
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Biometals 2002: Third International Biometals Symposium
Mercury resistance (mer) operons in enterobacteria 1 J. L. Hobman , A. M. M. Essa and N. L. Brown School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K.
Abstract GVhTkho hVi]ijRdTV ]i WekdU ]d cRdo ZVdVhR eW SRTjVh]R. 7ecced RcedZij VdjVheSRTjVh]R RhV jhRdifeiedi hVbRjVU je Ld10’ m[]T[ ]i Sej[ cVh( Tkh]T ]ed( RdU ijhVfjecoT]d(}ifVTj]decoT]d( RdU ikbf[edRc]UV(hVi]ijRdj. Ij[Vh Ld10(hVbRjVU jhRdifeiedi eWjVd [RlV U]ffVhVdj Rdj]S]ej]T hV( i]ijRdTVi TecfRhVU m]j[ Ld10’ Skj i[RhV cRdo ded(Rdj]S]ej]T(hVi]ijRdTV ZVdVi m]j[ ]j. Cd j[]i Rhj]TbV mV U]iTkii feii]SbV cVT[Rd]ici Weh j[V Vlebkj]ed eW Ld10RdU hVbRjVU ZVdVj]T VbVcVdji.
Introduction GVhTkho RdU cVhTkho(TedjR]d]dZ TecfekdUi’ ikT[ Ri cVhTkh]T T[beh]UV %BZ7b)’ cVhTkheki # T[beh]UV %BZ 7b ) RdU cVhTkh]T ikbf[]UV %BZK)’ # # [RlV SVVd kiVU Ri cVU]T]dVi Weh j[V jhVRjcVdj eW cRdo [kcRd R]bcVdji’ hRdZ]dZ Whec Tedij]fRj]ed je ik]d U]iVRiV’ Weh cRdo [kdUhVUi eW oVRhi [,]. GVhTkho mRi fVh[Rfi ceij WRcekibo %j[ekZ[ dej dVTViiRh]bo VffiTRT]ekibo) kiVU Weh j[V jhVRjcVdj eW iof[]b]i fh]eh je j[V iodj[Vi]i RdU kiV eW j[V phij iVbVTj]lV(jen]T]jo T[Vcej[VhRfVkj]T RZVdj’ Rhi( f[VdRc]dV %PKRblRhiRd ')’m[]T[ mRi U]iTelVhVU So PRkb 9[hb]T[ RdU KR[RT[]he BRjR ]d ,9)3. L[V fheUkTj]ed eW fVd]T]bb]d ]d bRhZV gkRdj]j]Vi Ukh]dZ j[V ,90)i RdU ]ji []Z[bo VffVTj]lV jhVRj( cVdj eW iof[]b]i ikfVhTVUVU Sej[ cVhTkh]Rbi RdU PKRblRhiRd .'BemVlVh’ cVhTkho(TedjR]d]dZ TecfekdUi ikT[ Ri cVhTkh]T T[beh]UV’ cVj[ob( cVhTkho’ f[VdobcVhTkho’ cVhShec]d RdU j[]( cVheiRb mVhV ij]bb ]d m]UVifhVRU kiV ]d [eif]jRbi kdj]b Rj bVRij j[V VRhbo ,93)i ]d j[V M.E.’ M.K.5.’ AVhcRdo RdU DRfRd [-]’ RdU bRjVh ]d ej[Vh Tekd( jh]Vi. CdUVVU’ cVhTkh]Rbi RhV ij]bb ]d kiV jeURo Ri fhViVhlRj]lVi ]d lRTT]dVi RdU iecV [VRbj[TRhV fheUkTji RdU ]d UVdjRb RcRbZRci.
r Mercury resistance (Hg ) in bacteria, and Tn21 r BZ ]iWekdU ]d cRdo ZVdVhR eW SRTjVh]R’ RdU [Ri SVVd WekdU ]d Vdl]hedcVdjRb’ TeccVdiRb RdU
Key words: composite transposon, pre-antibiotic era, Tn21. r Abbreviations used: Hg , mercury resistance; ORF, open reading frame. 1 To whom correspondence should be addressed (e-mail J.L.Hobman!bham.ac.uk).
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Tb]d]TRbbo ]cfehjRdj ]iebRjVi %hVl]VmVU ]d [..)] L[V r phij hVfehj eW BZ]d SRTjVh]R mRi RcedZDiEeTyld( IdIIuh EugPuh]d ,92)’ m[Vd GeehV [0] WekdU j[Rj cVhTkh]T iRbji kiVU je U]i]dWVTj TRjZkj kiVU ]d ikjkhVi mekbU dej ]d[]S]j Zhemj[ eWD.EugPuh ]iebRjVU Whec ikhZ]TRb mekdUi’ Skj mekbU ]d[]S]j j[V Zhemj[ eWD.EugPuhWhec ej[Vh iekhTVi. L[V r Te(jhRdiUkTj]ed eW Sej[ BZRdU fVd]T]bb]d hV( i]ijRdTV je iVdi]j]lVD.EugPuhmRi hVfehjVU 0 oVRhi r bRjVh [1]. BZmRi phij UVjVTjVU RcedZij Rdj]S]ej]T( hVi]ijRdj Tb]d]TRb VdjVheSRTjVh]R ]d ,923 [2]. KkS( iVgkVdj hViVRhT[ So ej[Vh Zhekfi %hVl]VmVU ]d [.’3)] i[emVU j[Rj j[V ZVdVj]T b]dkRZV SVjmVVd Rdj]S]ej]T hVi]ijRdTV RdU cVhTkho hVi]ijRdTV ]d VdjVheSRTjVh]R [RU eTTkhhVU fh]eh je j[V bRjV ,91)i ]d DRfRd. 5 Tb]d]TRb ijhR]d eWDTiSPlE ¯PlcPgieh]Z]dRbbo ]iebRjVU Rj j[]i j]cV ed j[V SRi]i eW ]ji ckbj]fbV Rdj]S]ej]T(hVi]ijRdTV f[VdejofV’ mRi ikS( iVgkVdjbo i[emd je TedjR]d R ckbj](hVi]ijRdTV fbRic]U’ HJ, %J,)).) J,)) TRhh]Vi’ RcedZij ej[Vh ZVdVj]T VbVcVdji’ jhRdifeied Ld10%hV( l]VmVU ]d [3)]. Ld10SVbedZi je j[V Ld2ikSZhekf eW j[V 7bRii CC jhRdifeiRSbV VbVcVdji. Cj ]i SekdUVU So .4 Sf]dlVhjVU hVfVRj 8H5 iVgkVdTVi’ RdU Vd( TeUVi R cVhTkho(hVi]ijRdTV %bPg) efVhed %bPg( CTP6A7A)’ jhRdifei]j]ed WkdTj]edi %gPhiceC RdUiceA)’ jme ej[Vh efVd hVRU]dZ WhRcVi %IJ;i)’ ugR1RdUiceM’ RdU hVi]ijRdTVi je ijhVfjecoT]d} ifVTj]decoT]d RdU ikbf[edRc]UVi [2]. L[ViV hV( i]ijRdTVi RdU ej[Vh IJ;i RhV TRhh]VU ed Rd ,,.) kS ]djVZhed’ R 8H5 VbVcVdj m]j[ j[V TRfRT]jo je RTgk]hV Rdj]S]ej]T(hVi]ijRdTV TRiiVjjVi %;]ZkhV ,; hVl]VmVU ]d [.’3]). Ld10RdU hVbRjVU VbVcVdji RhV []Z[bo ikT( TViiWkb RdU RhV fRdUVc]T. L[Vo RhV WekdU ]d Tb]d]TRb’ Vdl]hedcVdjRb RdU TeccVdiRb SRTjVh]R %hVl]VmVU ]d [3’4.)] L[]i kS]gk]jo RdU lRh]Vjo eW Ld10(hVbRjVU VbVcVdji [Ri ]d fRhj SVVd Rjjh]Sk( jVU So Ah]dijVU Vj Rb. [4] je j[V RTgk]i]j]ed eW j[V ]djVZhRiV ioijVc So Rd RdTVijeh eW Ld10VdRSb]dZ j[V hRf]U UVlVbefcVdj eW dVm jofVi eW jhRdifeied So ZVdV RTgk]i]j]ed. L[V ceij eSl]( eki RUlRdjRZV TedWVhhVU je Ld10RdU hVbRjVU jhRdifeiedi So j[V fhViVdTV eW j[V ]djVZhed ]i j[V RS]b]jo je RTgk]hV ckbj]fbV Rdj]S]ej]T hV( i]ijRdTVi. L[]i TRfRS]b]jo’ fRhj]TkbRhbo ]d cVU]( TRbbo ]cfehjRdj SRTjVh]R’ mekbU [RlV SVVd R
#2002 Biochemical Society
Biochemical Society Transactions (2002) Volume 30, part 4
cR‘eh WRTjeh ]d j[V ikTTVii eW j[]i jhRdifeiRSbV VbVcVdj. 5bj[ekZ[ j[V kiV eW cVhTkho(TedjR]d]dZ Rdj]( c]TheS]Rbi [Ri SVVd bRhZVbo U]iTedj]dkVU’ VnTVfj Weh ifVT]pT b]c]jVU kiVi dejVU VRhb]Vh ]d j[]i hVl]Vm’ j[VhV ]i Vl]UVdTV je i[em j[Rj cVhTkho( hVi]ijRdj SRTjVh]R mVhV ij]bb SV]dZ WekdU ]d [eif]jRb SRTjVh]Rb fefkbRj]edi’ RbSV]j Rj bemVh bVlVbi j[Rd SVWehV’ iVlVhRb oVRhi RWjVh j[V U]iTedj]dkRj]ed eW cVhTkh]Rb kiRZV ]d j[V [eif]jRbi [-]. GehV hVTVdj ikhlVoi eW SRTjVh]Rb fefkbRj]edi [RlV i[emd j[Rj bPgefVhedi’ fRhj]TkbRhbo j[eiV hVbRjVU je Ld10RhV fhViVdj ]d VdjVheSRTjVh]R Whec Sej[ [kcRdi RdU Rd]cRbi’ RdU RhV RiieT]RjVU m]j[ ]djVZhedi RdU
ckbj]fbV Rdj]S]ej]T hVi]ijRdTVi [3’.9] Ok$[biUehW RdU WV]UVcRdd [,)] WekdU j[Rj 4% eW 4)3 kdiVbVTjVU AhRc(dVZRj]lV Tb]d]TRb ]iebRjVi TebbVTjVU ]d ,942 Whec [eif]jRbi ]d 5kijh]R’ Km]jzVhbRdU RdU AVhcRdo [oSh]U]zVU je plV Ld10(hVbRjVU fheSVi’ ]dTbkU]dZ j[VIciI0ZVdV Whec j[V Ld10]djVZhed Cd- %iVV ;]ZkhV ,). 5 Wkhj[Vh ,,% eW j[V ]iebRjVi [oSh]U]zVU je SVjmVVd edV RdU Wekh Ld10(ifVT]pT fheSVi. 6Rii Vj Rb. 9[] VnRc]dVU j[V ]dT]UVdTV eW ]djVZhedi ]d Rl]Rd fRj[eZVd]TAhITPgiITiE Idil ]iebRjVU Whec U]iVRiVU fekbjho’ RdU WekdU j[Rj 2. %eW j[ViV ]iebRjVi TedjR]dVU j[V TbRii C( ]djVZhed(RiieT]RjVUiciI0RdUfEIΔA0ZVdVi’ RdU 1) %eW j[V ]iebRjVi TedjR]dVU j[V ZVdViiciI0
Figure 1 Genetic maps of Tn21[6] and Tn5075L. Brown and J.L. Hobman,(A. M. M.Essa, D.J. Julian, S.P. Kidd, N. unpublished work) The black boxes represent the Tn2138 bp inverted repeats. The gene functions formerRTPCADEare reviewed in [3,7], the transposition gene functions forres,tnpRandtnpAare reviewed in [7,8]. The possible role oftnpMis also discussed in [7]. Tn5075is flanked by the insertion sequence elements IS5075L and IS5075R, which are 99.53:% identical to each other. Integron genes areintI1, the integrase gene,attI, insertion site,aadA1, aminoglycoside adenylyltransferase gene,qacEΔ1, resistance to quaternary ammonium compounds andsulI, sulphonamide resistance. The integron contains an ORF of unknown function (orf5) and two IS elements, IS1326and IS1353, as well as integron transposition genestniAand the partially deletedtniB[7]. IR, inverted repeat.
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EEMA0fEIΔA0RdUbPgA’ Whec R Ld10(b]kV jhRdifeied %;]ZkhV ,). 8kh]dZ ]dlVij]ZRj]edi ed j[V ]dT]UVdTV eW f[Vdejof]TRbbo VnfhViiVU Rdj]( S]ej]T hVi]ijRdTV ]dA.IdilWhec j[V Zkj ¯ehR eW [VRbj[o im]dV’ KkdUV RdU Kùhkc [,,] WekdU j[Rj j[Vo TekbU ]UVdj]Wo R Ld10(hVbRjVU jhRdifeied Whec edV eW j[ViV ]iebRjVi So P7J Rcfb]pTRj]ed ki]dZ fh]cVhi UVi]ZdVU Weh Ld10( eh ]djVZhed( ifVT]pT ZVdVi. L[V jhRdifeied mRi TRhh]VU ed R iVbW(jhRdic]ii]SbV fbRic]U’ fJj,3’ m[]T[ TekbU jhRdiWVh je fRj[eZVd]TA.IdliDElbdcPlE PciPgiIE iVhelRhTyeTibugiubRdU dehcRb f]Z Zkj ¯ehR A.IdlikdUVh bRSehRjeho TedU]j]edi.
r Hg in‘ pre-antibioticera ’bacteria r FeZ]TRbbo’ edV mekbU VnfVTj j[Rj BZ]i R ZVdejofV j[Rj SRTjVh]R [RlV TRhh]VU Weh R Tedi]UVhRSbV fVh]eU eW j]cV’ Z]lVd j[Rj cVhTkho [Ri SVVd b]SVhRjVU ]dje j[V S]eif[VhV So ZVebeZ]TRb fheTViiVi Weh c]b( bVdd]R’ RdU [Ri SVVd kiVU Ri Rd Rdj](c]TheS]Rb eh cVU]T]dV Weh cRdo [kdUhVUi eW oVRhi. L[V GkhhRo TebbVTj]ed eW VdjVheSRTjVh]R TebbVTjVU Whec bRhZVbo Tb]d]TRb iekhTVi So 9. 8. A. GkhhRo SVjmVVd ,9,3 r RdU ,910 TedjR]di BZSRTjVh]R. W[Vd j[V iVRbVU SRTjVh]Rb TkbjkhVi j[Rj WehcVU j[V GkhhRo Teb( bVTj]ed mVhV efVdVU ]d ,94)’ j[Vo mVhV jVijVU Weh hVi]ijRdTV je R hRdZV eW Rdj]S]ej]Ti RdU [VRlo cVjRbi. L[VhV mVhV lVho WVm SRTjVh]R Whec j[V TebbVTj]ed j[Rj mVhV Rdj]S]ej]T(hVi]ijRdj %-}0.. mVhV fVd]T]bb]d(hVi]ijRdj’ 9}0.. mVhV jVjhRToTb]dV( hVi]ijRdj)’ bRhZVh dkcSVhi j[Rj mVhV hVi]ijRdj je TeffVh’ jVbbkh]jV RdU RhiVd]T’ RdU j[hVV ijhR]di r mVhV]UVdj]pVUj[RjmVhVBZ[,-.] WV hVTVdjbo TedphcVU j[Rj dedV eW j[V j[hVV r BZ SRTjVh]RmVhV Rdj]S]ej]T(hVi]ijRdj’ RdU UV( jVhc]dVU j[V 8H5 iVgkVdTVi eW j[VbPgefVhedi Whec Rbb j[hVV eW j[Vc’ RdU ¯Rdk]dZ 8H5 Whec edV eW j[Vc %5.G. G.9iiR’ 8.D. Dkb]Rd’ K..P E]UU’ H.F. 6hemd RdU D.F. BeScRd’ kdfkS( b]i[VU meh.)k 8H5 iVgkVdTV RdRboi]i [Ri i[emd j[Rj jme eW j[V GkhhRo TebbVTj]ed ]iebRjVi Ted( jR]dVUbPgefVhedi j[Rj mVhV9% ]UVdj]TRb jebPg jhRdifeiedi j[Rj [RlV SVVd fhVl]ekibo ]iebRjVU Whec Vdl]hedcVdjRb iRcfbVi %Ld4042RdU Ld4025; hVl]VmVU ]d [,.).] L[V j[]hU ]iebRjV %Whec GkhhRo ijhR]dA.IdilG2.0’ m[]T[ mRi eh]Z]dRbbo ]iebRjVU ]d eh SVWehV ,9.,) mRi9% ]UVdj]TRb je Ld10’ RTheii j[VbPgZVdVi RdU jhRdifei]j]ed WkdTj]edi’ Skj de ]djVZhed mRi fhViVdj. CdijVRU eW ugR1RdUiceM’ R i]dZbV IJ;’ugR-M’ mRi fhViVdj %;]ZkhV ,). L[V ej[Vh cR‘eh U]ffVhVdTV SVjmVVd Ld10RdU Ld4704mRi j[Rj Ld4704mRi ¯RdkVU So
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]diVhj]ed iVgkVdTV VbVcVdji %CK4704F RdU CK4704J ;.9]UVdj]TRb je VRT[ ej[Vh) j[Rj1. % mVhV 9,% ]UVdj]TRb je CK3210F RdU CK3210J j[Rj ¯Rdk j[V PThofj]T 'jhRdifeiRSbV VbVcVdj Ld3210Whec fbRic]U J31,. BemVlVh’ j[V []Z[Vij ]UVdj]jo je j[V Ld4074 bPgZVdVi’ jhRdifei( ]j]ed WkdTj]edi’ CK4704F RdU CK4074J mRi j[V cVhTkho(hVi]ijRdTV jhRdifeied ]UVdj]pVU ]d j[V fbRic]U fB7G,. L[]i fbRic]U 8H5 iVgkVdTV TecVi Whec j[V hVTVdjbo fkSb]i[VU ZVdecV iV( gkVdTV eW j[V ckbj]fbo UhkZ(hVi]ijRdjD.PciPgiIE iVhelRhTyeTiijhR]d 7L,4 [,0]’ m[]T[ [RU SVVd ]iebRjVU ]d j[V GVkedZ 8VbjR hVZ]ed eW N]VjdRc ]d ,9. Whec R ‘klVd]bV [kcRd WVcRbV. L[V Ld10ikSZhekf eW jhRdifeiRSbV VbVcVdji ]i ikTTViiWkb SVTRkiV j[Vo RhV eWjVd TRhh]VU ed Ted‘kZRj]lV fbRic]Ui’ TRd jhRdifeiV RdU [RlV j[V RS]b]jo je RTgk]hV Rdj]S]ej]T hVi]ijRdTVi. Ikh 8H5 iVgkVdTV URjR %5. G. G. 9iiR’ 8. D. Dkb]Rd’ K. .P E]UU’ H.F. 6hemd RdU D.F. BeScRd’ kdfkS( b]i[VU meh)k RdU j[Rj eW PRhk[]bb Vj Rb. [,0] ikZZVij j[Rj Ld10(hVbRjVU VbVcVdji cRo SV TecfedVdji eW Tecfei]jV jhRdifeiedi %;]ZkhV ,)’ m[VhV j[V Ld10 jhRdifeied ]i ¯RdkVU Rj Sej[ j[VbPgRdU jhRdi( feied VdUi So l]hjkRbbo ]UVdj]TRb CK VbVcVdji. 5i oVj ]j ]i dej kdemd [em m]UVifhVRU j[ViV VbVcVdji RhV RcedZij Ld10(jofVbPghVi]ijRdTVi’ Skj j[VhV ]i iecV Vl]UVdTV je ikZZVij j[Rj j[Vo RhV dej kdTecced %5.G. G.9iiR’ 8.D. Dkb]Rd’ K..P E]UU’ H.F. 6hemd RdU D.F. BeScRd’ kdfkS( b]i[VU meh)k.
References 1 Goldwater,L. J. (1972) Mercury: a History of Quicksilver, York Press, Baltimore 2 Porter,F. D., Silver, S., Ong, C. and Nakahara, H. (1982) Antimicrob. Agents Chemother.22, 852±858 3 Hobman,J. L. and Brown, N. L. (1997) in Metal Ions in Biological Systems (Sigel, A. and Sigel, H., eds), pp. 527–568, Marcel Dekker, New York 4 Moore,B. (1960) Lancet2, 453±458 5 Richmond,M. H. and John, M. (1964) Nature (London) 202, 1360±1361 6 Smith,D. H. (1967) Science156, 1114±1116 7 Liebert,C. A., Hall, R. M. and Summers, A. O. (1999) Microbiol. Mol. Biol. Rev.63, 507±522 8 Grinsted,J., de la Cruz, F. and Schmitt, R. (1990) Plasmid 26, 163±189 9 Bass,L., Liebert, C. A., Lee, M. D., Summers, A. O., White, D. G., Thayer, S. G. and Maurer, J. G. (1999) Antimicrob. Agents Chemother.43, 2925±2929 10 Zu$hlsdorf, M. T. and Weidemann, B. (1992) Antimicrob. Agents Chemother.36, 1915±1921 11 Sunde,M. and Sørum, H. (2001) Microb. Drug Resist.7, 191±196
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12 Hughes,V. M. and Datta, N. (1983) Nature (London)302, 725±726 13 Nikiforov,V. G., Bass, I. A., Bogdanova, E. S., Gorlenko, Zh. M., Kalyaeva, E. S., Kopteva, A. V., Lomovskaya, O. L., Minakhin, L. S., Minakhina, S. V., Mindlin, S. Z. et al. (1999) Molekulyarna Biologiya33, 44±50
14 Parkhill,J., Dougan, G., James, K. D., Thomson, N. R., Pickard, D., Wain, J., Churcher, C., Mungall, K. L., Bentley, S. D., Holden, M. T. G. et al. (2001) Nature (London)413, 848±852
Received 1 March 2002
Molecular evidence for the role of a ferric reductase in iron transport 1 A. T. McKie* , G. O. Latunde-Dada*, S. Miret*, J. A. McGregor*, G. J. Anderson, C. D. Vulpe, J. M. Wrigglesworth* and R. J. Simpson* *Division of Life Sciences, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NN, U.K.,Queensland Institue of Medical Research, P.O. Royal Brisbane Hospital, Hereston, Queensland 4029, Australia, andDepartment of Nutritional Sciences, University of California, Berkeley, CA, U.S.A.
Abstract 8keUVdRb TojeT[hecVF%8TojS) ]i R [RVc fhejV]d i]c]bRh je j[V TojeT[hecVF12, fhejV]d WRc]bo. 8TojS ]i []Z[bo VnfhViiVU ]d UkeUVdRb Shki[( SehUVh cVcShRdV RdU ]i ]cfb]TRjVU ]d U]VjRho ]hed RSiehfj]ed So hVUkT]dZ U]VjRho WVhh]T ]hed je j[V WVhheki Wehc Weh jhRdifehj l]R HhRcf-}87L, %U]lRbVdj(TRj]ed jhRdifehjVh ,)}8GL, %U]lRbVdj cVjRb(jhRdifehjVh ,.) L[V fhejV]d ]i VnfhViiVU ]d ej[Vh j]iikVi RdU cRo RTTekdj Weh WVhh]T hVUkTjRiV RTj]l]jo Rj ej[Vh i]jVi ]d j[V SeUo.
Background 7hRdV RdU []i TebbVRZkVi [,] ikZZVijVU j[V hebV eW R fbRicR(cVcShRdV hVUkTjRiV ]d ]hed kfjRkV RdU fhel]UVU Vl]UVdTV Weh U]WVhh]T jhRdiWVhh]d hV( UkTjRiV RTj]l]jo ]d c]TheiecVi ]iebRjVU Whec b]lVh. K]dTV j[]i j]cV R dkcSVh eW fbRicR(cVcShRdV hVUen fhejV]di j[Rj ifVT]pTRbbo hVUkTV WVhh]T ]hed T[VbRjVi [RlV SVVd ]UVdj]pVU Rj j[V cebVTkbRh bVlVb ]d oVRij Ri mVbb Ri fbRdj heeji [-±0]. L[]i fhel]UVU ijhedZ Vl]UVdTV Weh j[V hVgk]hVcVdj eW R hVUkTj]ed ijVf fh]eh je kfjRkV eW ]hed So oVRij RdU fbRdj TVbbi. L[V WVhh]T hVUkTjRiV fhejV]di UViTh]SVU ]d oVRij RdU fbRdj ioijVci SVbedZ je j[V iRcV WRc]bo Ri A9P,(f[en’ R cR‘eh TecfedVdj eW j[V hVif]hRjeho Skhij en]URiV WekdU ]d cRccRbi. Cd cRccRbi U]VjRho ]hed ]i RSiehSVU ]d j[V fhen]cRb ]djVij]dV’ cR]dbo ]d j[V UkeUVdkc RdU ]j mRi kdemd Weh cRdo oVRhi j[Rj WVhheki ]hed ]i SVjjVh RSiehSVU j[Rd WVhh]T. L[V U]iTelVho ]d ,93 j[Rj R cR‘eh
Key words: duodenal cytochromeb, intestine, neutrophil. Abbreviations used: Dcytb, duodenal cytochromeb; DCT, divalent-cation transporter. 1 To whom correspondence should be addressed (e-mail andrew.t.mckie!kcl.ac.uk).
#2002 Biochemical Society
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kfjRkV fRj[mRo Weh ]hed ]d j[V ]djVij]dV ]dleblVU R U]lRbVdj TRj]ed jhRdifehjVh [HhRcf-’ U]lRbVdj( TRj]ed jhRdifehjVh , %87L,) eh U]lRbVdj(cVjRb jhRdifehjVh ,] [1±3] ijhedZbo ikZZVijVU j[Rj WVh( heki ]hed mRi j[V fhVWVhhVU Wehc eW ]hed RSiehSVU RdU ZRlV Wkhj[Vh ]cfVjki je j[V iVRhT[ Weh R ]djVij]dRb WVhh]T hVUkTjRiV.
Characterization of a duodenal ferric reductase activity L[V fhViVdTV eW ikT[ R ikhWRTV WVhh]T hVUkTjRiV RTj]l]jo ]d j[V UkeUVdRb ckTeiR mRi phij UViTh]SVU So j[V Ched GVjRSeb]ic Ahekf Rj E]dZi 7ebbVZV FedUed iecV j]cV SVWehV j[V U]iTelVho eW 87L, [4’4R.] Cd j[Rj ijkUo ]j mRi i[emd j[Rj R WVhh]T hVUkTjRiV RTj]l]jo TekbU SV cVRikhVU Rj j[V ikhWRTV eW j[V UkeUVdRb ckTeiR. L[V hVUkTjRiV RTj]l]jo mRi ijhedZbo ij]ckbRjVU So [ofen]R RdU ]hed UVpT]VdTo’ Sej[ eW m[]T[ ij]ckbRjV ]dThVRiVU RSiehfj]ed eW U]VjRho ]hed. Cd RUU]j]ed ]j mRi WekdU j[Rj j[V RTj]l]jo mRi []Z[Vij ]d j[V UkeUVdkc RdU bemVij ]d j[V ]bVkc’ TecfRj]SbV m]j[ j[V fhepbV eW ]hed RSiehfj]ed RbedZ j[V Zkj’ m[]T[ ]i []Z[Vij ]d UkeUVdkc RdU bemVij ]d ]bVkc. 5jjVcfji je fkh]Wo j[V fhejV]d hVifedi]SbV Weh j[]i RTj]l]jo fhel]UVU Vl]UVdTV j[Rj j[V RTj]l]jo mRi RiieT]RjVU m]j[ R F(jofV [RVc fhejV]d j[Rj mRi ]cckdebeZ]TRbbo U]ij]dTj Whec j[V H58PB en]URiV AP9,(P[en ].[9 L[V fhejV]d mRi dVlVh ikTTViiWkbbo fkh]pVU ki]dZ S]eT[Vc]TRb jVT[d]gkVi Ri j[V [RVc mRi beij VRhbo ]d j[V fkh]pTRj]ed. A]lVd j[V hVRiedRSbV [ecebeZo SVjmVVd A9P,(f[en RdU j[V oVRij RdU fbRdj WVhh]T hVUkTjRiV fhejV]di edV c]Z[j [RlV VnfVTjVU j[V UkeUVdRb WVhh]T hVUkTjRiV je SV R [ecebeZeki fhejV]d. BemVlVh’ UVZVdVhRjV P7J RffheRT[Vi ki]dZ hVZ]edi eW []Z[ [ecebeZo SV( jmVVd j[V oVRijFCAZVdVi RdU A9P,(f[en mVhV
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