Osteoblasts response to anodized commercially pure titanium in vitro [Elektronische Ressource] / vorgelegt von Jun Chen

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Aus der Universitätsklinik für Zahn-, Mund- und Kieferheilkunde Tübingen Abteilung Poliklinik für Zahnärztliche Prothetik mit Propädeutik Ärztlicher Direktor: Prof. Dr. Heiner Weber Sektion für Werkstoffkunde und Technologie Leiter: Professor Dr. J. Geis-Gerstorfer Osteoblasts Response to Anodized Commercially Pure Titanium in vitro Inaugural-Dissertation Zur Erlangung des Doktorgrades Der Zahnheilkunde der Medizinischen Fakultät der Eberhard-Karls-Universität zu Tübingen Vorgelegt von Jun Chen aus Zhejiang /China 2005 Dekan: Professor Dr. C. D. Claussen 1. Berichterstatter: Professor Dr. J. Geis-Gerstorfer 2. Berichterstatter: Professor Dr. H. Wolburg To my families Content 1. Introduction ····························································································· 1 2. Literature Review ··················· 2 2.1 Titanium and titanium oxides ················································ 2 4 2.2 Titanium surface modifications ····························· 2.2.1 Physical techniques ······································································ 4 2.2.2 Chemical treatments ················· 5 2.2.3 Combination methods ·································································· 7 2.3 Effects of modified titanium surfaces on osteoblasts ···························· 9 9 2.3.
Publié le : samedi 1 janvier 2005
Lecture(s) : 86
Tags :
Source : W210.UB.UNI-TUEBINGEN.DE/DBT/VOLLTEXTE/2005/1692/PDF/REAKTION_VON_OSTEOBLASTEN_AUF_ANODISCHE_OXIDATION.PDF
Nombre de pages : 91
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Aus der Universitätsklinik für Zahn-, Mund- und Kieferheilkunde Tübingen
Abteilung Poliklinik für Zahnärztliche Prothetik mit Propädeutik Ärztlicher Direktor: Prof. Dr. Heiner Weber
Sektion für Werkstoffkunde und Technologie
Leiter: Professor Dr. J. Geis-Gerstorfer
Osteoblasts Response to Anodized Commercially Pure
Titaniumin vitro
Inaugural-Dissertation Zur Erlangung des Doktorgrades
Der Zahnheilkunde
der Medizinischen Fakultät der Eberhard-Karls-Universität zu Tübingen
Vorgelegt von
Jun Chen
aus
Zhejiang /China
2005
Dekan: 1. Berichterstatter: 2. Berichterstatter:
Professor Dr. C. D. Claussen
Professor Dr. J. Geis-Gerstorfer Professor Dr. H. Wolburg
To my families
Content
1.Introduction·····························································································1
2.LiteratureReview···················································································· 2
2.1 Titanium and titanium oxides································································2
2.2 Titanium surface modifications····························································· 4
2.2.1 Physical techniques ······································································4
2.2.2 Chemical treatments····································································5
2.2.3 Combination methods·································································· 7
2.3 Effects of modified titanium surfaces on osteoblasts···························· 9
2.3.1 Effects of physical characteristics·················································9
2.3.2 Effects of chemical alterations on titanium surfaces·····················11
2.4 Osteoblast responses to modified titanium··········································· 13
2.4.1 Osteoblast···················································································· 13
2.4.2 Ostoeblast responses to titanium and its modifications················ 14
2.4.2.1 Cytotoxicity········································································ 14
2.4.2.2 Cell attachment and spreading·········································· 16
2.4.2.3 Cell proliferation and differentiation··································· 23
2.5 Anodic oxidation on commercial pure titanium········ ·············27 · ················
3. Aims of the present study ·······································································31
4.MaterialsandMethods············································································32
4.1 Anodized titanium specimens······························································· 32
4.1.1 Specimens preparation··································· 32 ·······························
4.1.2 Surface characterization································································ 33
4.1.2.1 Surface topography····························································
4.1.2.2 Surface roughness·····························································
4.1.2.3 Wettability···········································································
4.1.2.4 Chemical compositions······················································
33
38
40
42
Acknowledgments·······················································································85
Resume························ ················86 ································································ ·
8.References·······························································································74
Publications ·································································································84
6.4 Cell proliferation and differentiation······················································ 70
7. Conclusion·······························································································72
6.2 Cytotoxicity assay················································································· 65
6.3 Cell attachment and spreading···· ········· 66 ················································
6.Discussion·······························································································64
6.1 Experiments design·············································································· 64
5.5 Cell proliferation (determination of cell numbers)··································61
5.6 Alkaline phosphatase (ALP) activity····················································· 63
5.3 Cellular morphology·············································································· 53
5.4 Cytoskeleton organization····································································56
5.Results····································································································· 49
4.2.6 Statistical analysis········································································48
5.2 Cell attachment and spreading····························································· 50
5.1 Cytotoxicity··························································································· 49
4.2.3 Cell attachment and spreading·····················································44
4.2.2 Cytotoxicity assay········································································· 43
4.2.5 Alkaline phosphatase activity·······················································47
4.2.4 Cell proliferation (determination of cell numbers)························· 47
4.2 Osteoblasts cell culture················································· ········ 3 ················4
4.2.1 Osteoblast-like cell line···································· · 43 ····························
1. Introduction
 Unalloyed and alloyed titanium has been widely used to construct dental
implants because of its good biocompatibility. Rapid achievement of a stable
osseointegration between implant and bone tissues of the host is the main aim
in implant development. Because surface properties and/or chemical
composition play a critical role in achieving successful osseointegration, many
efforts have been employed to modify titanium surfaces and to improve its
biocompatibility.
 Anodic oxidation is an electrochemical method, and it is easy to produce
various oxide layers on titanium surfaces by adjusting the anodizing conditions,
such as anodizing electrolytes, temperature, anodizing voltage, and so on.
However, there are many kinds of combinations of the above parameters, so it
is difficult to find out the best combination. Some efforts focused onin vivo
experiments to investigate bone tissue response to anodized titanium implants,
while few articles investigated the basic responses of osteoblasts to these
surfaces. Osteoblasts play a critical role at the interface between implants and
bone tissue, so it is necessary to study cell behavior of osteoblasts cultured
directly on anodized titanium surfaces.
 The aim of this study was to evaluate human osteoblast responses to anodic
oxides of titaniumin vitro. Two kinds of anodizing electrolytes with different
composition and a series of anodizing voltages were adopted. In the present
study, cell cultures of the osteoblast-like cell line SaOS-2, derived from human
osteoblastic sarcoma, were performed on anodized titanium surfaces, and
cytotoxicity, cell attachment and spreading, cell morphology, cell proliferation
and differentiation were assessed.
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