Architectures of DNA block copolymers [Elektronische Ressource] / vorgelegt von Ke Ding

Dingke

Description

Architectures of DNA block copolymers DISSERTATION zur Erlangung des Grades "Doktor der Naturwissenschaften" am Fachbereich Chemie, Pharmazie und Geowissenschaften der Johannes Gutenberg-Universität Mainz vorgelegt von Master-Chem. Ke Ding geb. in 03.01.1978 aus Jiangxi (V. R. China) Mainz ，2006 Tag der mündlichen Prüfung: 14. 11. 2006ABSTRACT The aim of this work is to understand the surface morphology of amphiphilic deoxyribonucleic acid (DNA) block copolymers, which is an interesting topic in DNA nanotechnology. The specific base pairing in DNA sequences makes DNA programmable, which enables the transition from 1- dimensional structure to 2- or 3- dimensional structures of DNA block copolymers. The atomic force microscopy (AFM) has been applied for investigating the different architectures formed on different substrates in liquid and/or in air. In-situ AFM studies illustrated that both DNA-b-polypropylene oxide (PPO) and DNA-b-polystyrene (PS) exhibit as single molecules or dimers of them below the critical micelle concentration (CMC) and globular micelles above the CMC. The micelles of DNA-b-PPO are flattened on mica surface with a height of 4-6 nm by AFM measurements due to the adsorption of the DNA with mica through the positive ions in buffer solutions, which was proved by dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS) to have a diameter around 11 nm in solution.

Sujets

• Biologie

Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2006
• Nombre de lectures: 36
• Langue: English
• Poids de l'ouvrage: 7 Mo

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