Glycohistochemical, immunohistochemical and ultrastructural studies of the bovine epididymis [Elektronische Ressource] / by Alkafafy Mohamed

Alkafafy, Mathilde Mohamed

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2005
• Nombre de lectures: 21
• Langue: Deutsch
• Poids de l'ouvrage: 6 Mo

Extrait

Aus dem Institut für Tieranatomie der
Ludwig-Maximilians-Universität München
Lehrstuhl für Tieranatomie II
insbesondere Allgemeine Anatomie, Histologie und Embryologie
Vorstand: Prof. Dr. Dr. Dr. habil. F. Sinowatz




Under Supervision of
Prof. Dr. Dr. Dr. habil. F. Sinowatz,
Head of Institute of Veterinary Anatomy II
Ludwig-Maximilians-Universität, München



Glycohistochemical, Immunohistochemical and Ultrastructural
Studies of the Bovine Epididymis


A thesis
submitted for the
Doctor Degree in Veterinary Medicine
Faculty of Veterinary Medicine
Ludwig-Maximilians-Universität, München

By
Alkafafy Mohamed
From
Dakahlia-Egypt



Munich 2005Gedruckt mit Genehmigung der Tierärztlichen Fakultät der
Ludwig-Maximilians-University Munich













Dekan: Univ.-Prof. Dr. A. Stolle
Referent: Univ.-Prof. Dr. Dr. F. Sinowatz
Korreferent: Univ.-Prof. Dr. J. Hirschberger



Tag der Promotion: 11. Febraur 2005























To
My Parents, Wife and Kids



























Contents

1 INTRODUCTION --------------------------------------------------------------------------------------------------------------------------- 1
2 REVIEW OF LITERATURE -------------------------------------------------------------------------------------------------------------- 2
2.1 DEVELOPMENT OF THE EPIDIDYMIS----------------------------------------------------------------------------------- 2
2.1.1 Prenatal Development ------------------------------------------------------------------------------------------- 2
2.1.2 Postnatal Development ------------------------------------------------------------------------------------------ 4
2.2 MACROSCOPICAL ANATOMY AND SPECIES DIFFERENCES----------------------------------------------------------- 5
2.2.1 General view ------------------------------------------------------------------------------------------------------ 5
2.2.2 Species differences------------------------------------------------------------------------------------------------ 5
2.2.3 Blood supply of the epididymis---------------------------------------------------------------------------------- 7
2.2.4 Lymphatic drainage ---------------------------------------------------------------------------------------------- 8
2.2.5 Nerve supply------------------------------------------------------------------------------------------------------- 9
2.3 LIGHT HISTOLOGICAL STRUCTURE OF MAMMALIAN EPIDIDYMIS------------------------------------------------- 9
2.3.1 General view 9
2.3.2 Light Histological Structure of the Bovine Epididymis -----------------------------------------------------11
2.3.2.1 Efferent ductules (ED)---------------------------------------------------------------------------------------------------12
2.3.2.2 Epididymal duct ----------------------------------------------------------------------------------------------------------13
2.3.2.2.1 Segment I------------------------------------------------------------------------------------------------------------13
2.3.2.2.2 Segment II-----------------------------------------------------------------------------------------------------------14
2.3.2.2.3 Segment III15
2.3.2.2.4 Segment IV ---------------------------------------------------------------------------------------------------------15
2.3.2.2.5 Segment V16
2.3.2.2.6 Segment VI16
2.4 LECTIN HISTOCHEMISTRY ---------------------------------------------------------------------------------------------17
2.4.1 Definition of a lectin17
2.4.2 Common structure and molecular properties of lectins-----------------------------------------------------17
2.4.3 Biological properties of lectins --------------------------------------------------------------------------------18
2.4.4 Lectin toxicity ----------------------------------------------------------------------------------------------------20
2.4.5 Classification of lectins -----------------------------------------------------------------------------------------20
2.4.6 Nomenclature of lectins21
2.4.7 Endogenous Lectins22
2.4.8 Applications of lectins-------------------------------------------------------------------------------------------23
2.4.9 Application of lectins for the characterization of the epididymis------------------------------------------24
2.5 ELECTRON MICROSCOPIC STRUCTURE OF THE EPIDIDYMIS --------------------------------------------------------25
2.5.1 General view -----------------------------------------------------------------------------------------------------25
2.5.2 Species specific differences-------------------------------------------------------------------------------------25
2.5.3 Electron microscopic structure of the bovine epididymis---------------------------------------------------37
2.5.3.1 Efferent ductules----------------------------------------------------------------------------------------------------------37
2.5.3.2 Epididymal duct39
2.5.3.2.1 Segment I------------------------------------------------------------------------------------------------------------39
2.5.3.2.2 Segment II-----------------------------------------------------------------------------------------------------------41
2.5.3.2.3 Segment III42
2.5.3.2.4 Segments IV and V ------------------------------------------------------------------------------------------------43
2.5.3.2.5 Segment VI ---------------------------------------------------------------------------------------------------------44
2.5.4 Extraepithelial, peritubular and interstitial structures -----------------------------------------------------45
2.6 EPIDIDYMAL PHYSIOLOGY --------------------------------------------------------------------------------------------47
3 MATERIAL AND METHODS52
3.1 MATERIALS -------------------------------------------------------------------------------------------------------------52
3.1.1 Animals52
3.1.1.1 Adult specimens ----------------------------------------------------------------------------------------------------------52
3.1.1.2 Foetal specimens53
3.2 METHODS ---------------------------------------------------------------------------------------------------------------53
3.2.1 Light microscopy-------------------------------------------------------------------------------------------------53
3.2.1.1 Fixation, Processing and Sectioning -----------------------------------------------------------------------------------53
3.2.1.1.1 Paraffin sections----------------------------------------------------------------------------------------------------53
3.2.1.1.2 Frozen sections -----------------------------------------------------------------------------------------------------54
3.2.1.2 Histological Staining54
3.2.1.2.1 Conventional staining ---------------------------------------------------------------------------------------------54
3.2.1.2.2 Lectin histochemistry (Glycohistochemistry) ------------------------------------------------------------------54
3.2.1.2.3 Immunohistochemical staining56
3.2.2 Transmission electron microscopy ----------------------------------------------------------------------------58
3.3 Chemicals -----------------------------------------------------------------------------------------------------------58
iContents

4 RESULTS------------------------------------------------------------------------------------------------------------------------------------60
4.1 PRENATAL DEVELOPMENT OF THE BOVINE EPIDIDYMIS------------------------------------------------------------60
4.1.1 Microscopic anatomy of the bovine prenatal epididymis ---------------------------------------------------60
4.1.1.1 The third gestational month ---------------------------------------------------------------------------------------------60
4.1.1.2 The fourth gestational month -------------------------------------------------------------------------------------------61
4.1.1.3 The fifth gestational month63