sP (EO-stat-PO) - glycosaminoglycans (GAGs) hybrid-hydrogels for medical applications [Elektronische Ressource] / Anandhan Dhanasingh

Anandhan Dhanasingh

Informations

• Publié par: rheinisch-westfalischen_technischen_hochschule_-rwth-_aachen
• Publié le: 01 janvier 2012
• Nombre de lectures: 91
• Langue: English
• Poids de l'ouvrage: 3 Mo

Extrait

sP (EO-stat-PO) - Glycosaminoglycans
(GAGs) Hybrid-Hydrogels for Medical
Applications





Von der Fakultät für Mathematik, Informatik und Naturwissenschaften der
RWTH Aachen University zur Erlangung des akademischen Grades eines
Doktors der Naturwissenschaften genehmigte Dissertation


vorgelegt von


Anandhan Dhanasingh, B.E., M.Sc.,

aus Chennai, Indien




Berichter: Univ.Prof. Dr. Martin Möller
Prof. Dr. Jürgen Groll


Tag der mündlichen Prüfung: 01.12.2011







Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online verfügbar.
























-ஔவையா

What has learnt is a handful, and what has still to acquire is wide as the world

- Auvaiyar
th (Before 9 century, India)
















ர்Table of Contents
Table of Contents
Acknowledgement...................................................................................................... ii
Summary..................................................................................................................... iv
List of abbreviations................................................................................................... vii

1. Introduction
1.1 Hydrogels as DDS and TE Scaffolds................................................................. 1
1.2 Description of this thesis.................................................................................... 3
1.3 References............. 4

2. Literature Overview
2.1 Hydrogel........................................................................................................... 5
2.1.1 Introduction.................................................................................................. 5
2.1.2 Classification of hydrogels........................................................................... 5
2.1.3 Properties of hydrogels................................................................................. 8
2.1.4 Classification of water in hydrogels............................................................. 11
2.1.5 Common applications of hydrogels.............................................................. 12
2.1.6 Summary of the important physico-chemical parameters and properties
of hydrogels in general 13

2.2 Hydrogels as biomedical materials
2.2.1 Introduction................................................................................................ 14
2.2.2 Hydrogels in drug delivery system............................................................. 15
2.2.3 Hydrogels as tissue engineering scaffolds.................................................. 19

2.3 Extra-cellular matrix (ECM)
2.3.1 Introduction................................................................................................ 22
2.3.2 Classes of ECM.......................................................................................... 23
2.3.3 Component of CM...................................................................................... 23

2.4 GAGs and their importance in connective tissue/ECM
2.4.1 Introduction................................................................................................ 24
2.4.2 Classification of GAGs............................................................................... 24
2.4.3 Properties of GAGs.................................................................................... 25
2.4.4 Proteoglycans.............................................................................................. 26
2.4.5 Hyaluronan...................... 27

2.5 References......................................................................................................... 38

3 NCO-sP(EO-stat-PO) as cross-linker to HA
3.1 Introduction...................................................................................................... 43
3.2 Materials........................................................................................................... 44
3.3 Hydrogels preparation.... 44
3.4 Contact angle measurements............................................................................ 45
3.5 Equilibrium water content measurements........................................................ 45
3.6 Differential scanning calorimetry..................................................................... 46
3.7 FT-IR spectroscopy.......................................................................................... 46
3.8 Cryo-FESEM................. 46
3.9 Rheological analysis......................................................................................... 46
3.10 In-vitro stability test in PBS............................................................................. 47

Table of Contents
3.11 Enzymatic degradation.................................................................................... 47
3.12 Statistical nalysis.............................................................................................. 47
3.13 Results and discussions.................................................................................... 48
3.14 Conclusion........................................................................................................ 58
3.15 References...................... 60

4 Comparison of sP(EO-stat-PO) with isocyanate and acrylate end groups
as respective cross-linker for unmodified and thiol modified
Hyaluronic acid
4.1 Introduction...................................................................................................... 62
4.2 Materials........................................................................................................... 63
4.3 Hydrogel preparation........................................................................................ 63
4.4 Contact angle measurements............................................................................ 64
4.5 Equilibrium water content measurements........................................................ 64
4.6 Differential scanning calorimetry..................................................................... 65
4.7 FT-IR spectroscopy.......................................................................................... 65
4.8 RAMAN spectroscopy... 65
4.9 Cryo-FESEM................. 65
4.10 Rheological analysis......................................................................................... 65
4.11 In-vitro stability in PBS.................................................................................... 66
4.12 Enzymatic degradation..................................................................................... 66
4.13 Statistical nalysis.............................................................................................. 66
4.14 Results and discussion...................................................................................... 67
4.15 Conclusion........................................................................................................ 77
4.16 References........................................................................................................ 78

5 NCO-sP(EO-stat-PO) as universal cross-linker to GAGs
5.1 Introduction...................................................................................................... 81
5.2 Materials........................................................................................................... 82
5.3 Hydrogel preparation........................................................................................ 82
5.4 Contact angle measurements............................................................................ 83
5.5 Equilibrium water content measurements......................................................... 83
5.6 FT-IR spectroscopy........................................................................................... 83
5.7 Cryo-FESEM.................................................................................................... 83
5.8 Rheological analysis.... 84
5.9 In-vitro stability test in PBS............................................................................. 84
5.10 Results and discussions.................................................................................... 84
5.11 Conclusion........................................................................................................ 90
5.12 References....................................................................