Synthesis, characterization, and biological evaluation of gelatin-based scaffolds [Elektronische Ressource] / von Giuseppe Tronci

Giuseppe Tronci

Sujets

• Biologie

Informations

• Publié par: universitat_potsdam
• Publié le: 01 janvier 2010
• Nombre de lectures: 21
• Langue: English
• Poids de l'ouvrage: 17 Mo

Extrait

Aus dem Zentrum für Biomaterialentwicklung, Institut für Polymerforschung,
GKSS Forschungszentrum GmbH


Synthesis, Characterization, and
Biological Evaluation of
Gelatin-based Scaffolds

Dissertation

zur Erlangung des akademischen Grades
"doctor rerum naturalium"
(Dr. rer. nat.)
in der Wissenschaftsdisziplin
„Materialien in den Lebenswissenschaften“

eingereicht an der
Mathematisch-Naturwissenschaftlichen Fakultät
der Universität Potsdam

von

Giuseppe Tronci
aus Lecce, Italien

Potsdam, 2010 This work is licensed under a Creative Commons License:
Attribution - Noncommercial - Share Alike 3.0 Unported
To view a copy of this license visit
http://creativecommons.org/licenses/by-nc-sa/3.0/






































Published online at the
Institutional Repository of the University of Potsdam:
URL http://opus.kobv.de/ubp/volltexte/2011/4972/
URN urn:nbn:de:kobv:517-opus-49727
http://nbn-resolving.org/urn:nbn:de:kobv:517-opus-49727



















Gutachter: Prof. A. Lendlein, Universität Potsdam
Prof. R. v. Klintzing, Technische Universität Berlin
Prof. M. Maskos, Bundesanstalt für Materialforschung und -prüfung


Tag der Annahme der Dissertation: 07.07.2010
Tag der Disputation: 15.12.2010

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This work was financed by the German Research Foundation (Deutsche
Forschungsgemeinschaft (DFG)) through the Collaborative Research Center 760 (CRC – 760,
Sonderfoschungsbereich 760 (SFB 760)), subproject B5.


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“Research is to see what everybody else has seen, and to
think what nobody else has thought.”

Albert von Szent-Györgyi de Nagyrápolt

































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Statement of Originality

I, Giuseppe Tronci, formally submit the dissertation entitled “Synthesis, Characterization and
Biological Evaluation of Gelatin-based Scaffolds” to the University of Potsdam, Faculty of
Mathematics and Natural Sciences, Germany, for the acquirement of the academic degree of
Doctor of natural sciences (Dr. rer. nat.) in Materials for Life Sciences. The work presented
here was carried out from March 2006 to July 2010 at the Center for Biomaterial
Development, Institute for Polymer Research, GKSS Research Center, campus Teltow.

I hereby certify that this submission is entirely my own original work and that, to the best of
my knowledge and belief, it contains no material previously published or written by another
person, except where due reference is made in the thesis itself. Neither the dissertation, nor
any sections thereof, has been previously submitted for a degree or other qualification to any
other University or Institution. Any contribution made to the research by others, with whom I
have worked at GKSS or elsewhere, is explicitly acknowledged in the thesis.

Giuseppe Tronci
Potsdam, 07.07.2010
vii
Contents
Contents

List of abbreviations................................................................................................................. xii
Abstract .................................................................................................................................. xvii
Zusammenfassung................................................................................................................... xix
Acknowledgements ................................................................................................................ xxii
1. Introduction....- 1 -
1.1 Clinical need of bone regeneration.............................................................................. - 1 -
1.2 The extracellular matrix .............................................................................................. - 4 -
1.3 Regenerative Medicine................................................................................................ - 5 -
1.4 Hydrogels for Regenerative Medicine ........................................................................ - 7 -
1.5 Collagen ...................................................................................................................... - 9 -
1.6 Gelatin ....................................................................................................................... - 11 -
1.7 Gelatin hydrogels: state of the art and challenges..................................................... - 13 -
1.8 Design of degradable and form-stable hydrogels...................................................... - 15 -
2. Aim of the Ph. D. thesis ...............................................................................................- 16 -
3. Strategies and concept..................................................................................................- 18 -
4. Entropy-elastic gelatin-based hydrogels: synthesis and characterization ....................- 23 -
4.1 Networks synthesized in DMSO............................................................................... - 23 -
4.1.1 Investigation of crystallinity in Networks synthesized in DMSO using
WAXS .......................................................................................................................... - 25 -
4.1.2 Investigation of Swelling behavior of Networks synthesized in DMSO ............ - 26 -
4.1.3 Investigation of Mechanical Properties of Networks synthesized in DMSO
using tensile tests.......................................................................................................... - 27 -
4.2 Networks synthesized in water.................................................................................. - 28 -
viii
Contents
4.2.1 Investigation of crystallinity in Networks synthesized in water using
WAXS .......................................................................................................................... - 30 -
4.2.2 Swelling behavior of Networks synthesized in water ......................................... - 32 -
4.2.3 Mechanical properties of Networks synthesized in water................................... - 34 -
4.2.4 Investigation of Thermal properties of Networks synthesized in water.............. - 37 -
4.3 Analysis of the crosslinking reaction ........................................................................ - 39 -
4.4 Summary ................................................................................................................... - 43 -
5. Design of a gelatin-based scaffold system ...................................................................- 45 -
5.1 The integrated foaming-crosslinking process ........................................................... - 45 -
5.2 Foam formation and stabilization.............................................................................. - 48 -
5.3 Analysis of the porous structure................................................................................ - 52 -
5.4 Gelatin chain helicity investigated by WAXS .......................................................... - 55 -
5.5 Thermal properties determined by TGA and DSC.................................................... - 57 -
5.6 Dry-state mechanical properties - 59 -
5.6.1 Shape recovery of freeze-dried gelatin scaffold.................................................. - 61 -
5.7 Summary ................................................................................................................... - 63 -
6. Characterization of gelatin-based scaffolds in aqueous environments ........................- 65 -
6.1 Swelling behaviour of gelatin-based scaffolds in water............................................ - 65 -
6.2 Form-stability of gelatin-based scaffolds.................................................................. - 67 -
6.2.1 Water uptake in cell culture medium - 70 -
6.3 Macroscopic elasticity............................................................................................... - 72 -
6.3.1 Macro-mechanical properties by compression tests............................................ - 74 -
6.4 Micro-mechanical properties by AFM...................................................................... - 75 -
6.5 Hydrolytic degradation...................................................................