Roche Applied Science Launches the E-Plate Insert for Analysis of Cell-Cell Interactions using the xCELLigence System

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• Publié par: roche-diagnostics-gmbh
• Nombre de lectures: 10
• Langue: English

Extrait

Roche Applied Science Launches the E-Plate

Insert for Analysis of Cell-Cell Interactions

using the xCELLigence System

PR Newswire

PENZBERG, Germany, June 13, 2012

PENZBERG,

Germany

,

June 13, 2012

/PRNewswire/ --

The co-cultivation of two different cell types is an important way to investigate

cell-cell interactions and communication.

Co-cultivation can be used in a wide variety of applications, including cell

signalling and toxicology testing. A key goal in drug discovery is to identify how

to most effectively monitor cell-cell interactions when cells show pathology.

Roche Applied Science is now providing the new E-Plate Insert 16 and E-Plate

Insert 96, permitting continual impedance-based monitoring of indirect cell-cell

interactions/communication using xCELLigence Instruments.

In a recent study at the INRS-Institut Armand-Frappier (

Laval, Quebec, Canada

)

the E-Plate Insert had been used to test the behaviour of BeWo human

placental choriocarcinoma cells in co-culture with H295R human adrenocortical

carcinoma cells. "Using the E-Plate Insert in our co-culture experiments

requires fewer manipulations and allows us to perform several experiments

simultaneously - hormone measurements, signalling pathway analysis, real-

time monitoring of biochemical and morphological differentiation of the cells,"

summarized Prof. Thomas Sanderson and Prof. Cathy Vaillancourt, who are co-

investigators on this project and co-supervisors of Andrée-Anne Hudon

Thibeault, the Masters student who realised the study. The Canadian group

plans to use the unique abilities of the co-culture-compatible E-Plate Insert and

xCELLigence cell response monitoring technology, to determine the effect of

co-culture on primary villous trophoblast homeostasis (polarization,

differentiation/syncytialization, fusion, apoptosis) and on H295R cells

(proliferation, viability, apoptosis) as well as expression and activity levels of key

placental and fetal adrenal steroidogenic enzymes.

The membrane-containing E-Plate Inserts enable sensitive, reproducible

measurement of indirect cell-to-cell interactions in real-time, and in a label-free

environment using the xCELLigence Instruments (RTCA DP, SP, and MP

Analyzers). Combined with microscopy-compatible E-Plates VIEW, E-Plate

Inserts 16 and 96 are available for low to high throughput applications.

Impedance-based measurements are particularly advantageous when co-

culturing cells for pinpointing when and how cells respond to each other, thus

identifying the best time points for further experiments.

About Roche

Headquartered in

Basel, Switzerland

, Roche is a leader in research-focused

healthcare with combined strengths in pharmaceuticals and diagnostics. Roche

is the world's largest biotech company with truly differentiated medicines in

oncology, virology, inflammation, metabolism and central nervous system

(CNS). Roche is also the world leader in in-vitro diagnostics, tissue-based cancer

diagnostics and a pioneer in diabetes management. Roche's personalized

healthcare strategy aims at providing medicines and diagnostic tools that

enable tangible improvements in the health, quality of life and survival of

patients. In 2011, Roche had over 80,000 employees worldwide and invested

over

8 billion Swiss francs

in R&D. The Group posted sales of

42.5 billion Swiss

francs

. Genentech,

United States

, is a wholly owned member of the Roche

Group. Roche has a majority stake in Chugai Pharmaceutical,

Japan

. For more

information: http://www.roche.com.

XCELLIGENCE is a trademark of Roche.

E-PLATE and ACEA BIOSCIENCES are registered trademarks of ACEA

Biosciences, Inc. in the US.

For life science research only. Not for use in diagnostic procedures

For further information please contact:

Roche Diagnostics

Dr. Burkhard Ziebolz

Phone: [phone]

Email: [e-mail]