Thesis presented to obtain the degree of Docteur de l'Université Louis Pasteur

Albert - Ludwigs Universitat

Description

Niveau: Supérieur, Doctorat, Bac+8
Thesis presented to obtain the degree of Docteur de l'Université Louis Pasteur STRASBOURG I & Doktor der Naturwissenschaften Der Albert-Ludwigs Universität FREIBURG NEUROSCIENCES Effets in vitro et in vivo d'une application combinée de 3,4-methylènedioxymethamphetamine (MDMA, ecstasy) et d'éthanol : étude des auto- et hétérorécepteurs présynaptiques du sous-type 5-HT1B et de leur surexpression par transfection virale de gène. Effects of combined applications of ethanol and 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) in vitro and in vivo: focus on presynaptic 5-HT1B auto- and heteroreceptors and their possible overexpression using HSV-1-mediated gene transfer. RIEGERT Céline Defended in public on December 12th, 2007 Referees: Dr CASSEL JC LINC, Strasbourg Thesis Director Prof Dr FISCHBACH KF Faculty of Biology, Freiburg Thesis co-Director Prof Dr JACKISCH R Pharmacology, Freiburg Thesis co-Director Dr LARMET Y ULP, Strasbourg Internal referee Dr MORMEDE P INRA, Bordeaux External referee Prof Dr AKTORIES K Pharmacology, Freiburg External referee

• director prof


• surexpression par transfection virale de gène


• freiburg external


• doktor der


• strasbourg internal referee


• thesis presented

Sujets

• Louis Pasteur University
• Biology

Informations

• Publié par: profil-zyak-2012
• Nombre de lectures: 14
• Langue: English
• Poids de l'ouvrage: 19 Mo

Extrait

Thesis presented to obtain the degree of
Docteur de l’Université Louis Pasteur
STRASBOURG I
&
Doktor der Naturwissenschaften
Der Albert-Ludwigs Universität
FREIBURG

NEUROSCIENCES

Effets in vitro et in vivo d’une application combinée de
3,4-methylènedioxymethamphetamine (MDMA, ecstasy) et d’éthanol :
étude des auto- et hétérorécepteurs présynaptiques du sous-type 5-HT 1B
et de leur surexpression par transfection virale de gène.

Effects of combined applications of ethanol and
3,4-methylenedioxymethamphetamine (MDMA, ecstasy) in vitro and in vivo: focus on
presynaptic 5-HT auto- and heteroreceptors and their possible overexpression 1B
using HSV-1-mediated gene transfer.

RIEGERT Céline

thDefended in public on December 12 , 2007

Referees:
Dr CASSEL JC LINC, Strasbourg Thesis Director
Prof Dr FISCHBACH KF Faculty of Biology, Freiburg Thesis co-Director
Prof Dr JACKISCH R Pharmacology, Freiburg Thesis co-Director
Dr LARMET Y ULP, Strasbourg Internal referee
Dr MORMEDE P INRA, Bordeaux External referee
Prof Dr AKTORIES K Pharmacology, Freiburg External referee









A mes parents
A ma sœur, ma petite



ACKNOWLEDGEMENTS
SSppeecciiaall tthhaannkkss ttoo…… SSppeecciiaall tthhaannkkss ttoo……

I first would like to thank Dr. Larmet (Strasbourg) and Dr. Mormede (Bordeaux) as well
as Prof. Dr. Fischbach (Freiburg) and Prof. Dr. Aktories (Freiburg) for accepting to be
members of the jury of my thesis. My special thanks also to Prof. Dr. Fischbach for
accepting to be my thesis director at the Faculty of Biology at the University of
Freiburg.
I would like to thank the neuroscience association Neurex for their three years of
financial support and interest in my work. This work was additionally funded by the
European Doctoral College and the University of Freiburg.

I would also like to thank Prof. Will and Dr. Kelche as well as Prof. Starke and Prof.
Hein for welcoming me in their lab, respectively, in Strasbourg and Freiburg. They
offered me all the facilities necessary to realize this work.

My very special thanks go to Dr. Cassel, director of this project, for his engagement.
His patience and comprehensiveness, strong experience and extremely accurate view
on scientific research were indispensable for me during the last five years we worked
together.

I don’t forget the co-director of my project, Prof. Dr. Jackisch for our every-day
collaboration in the lab. I am very grateful for his numerous advices and his will to
share his long experience in the neuroscience research as well as for his great help in
all aspects of my work.

I would like to acknowledge Prof. Neumaier and all his team for our collaboration on the
‘virus project’.

I would also like to thank Dr. Leemhuis for his help on the confocal microscope. He
allowed me to have beautiful pictures of neurons!

I also take the opportunity to thank all the co-workers from the ‘anatomy lab’ from Prof.
Dr. Frotcher. Special thanks go to Ulrich, Imre, Margot, Shanting and Sandra who
shared with me their “cell culture lab” and a lot of essential advices.

I ACKNOWLEDGEMENTS
I would also like to thank all the persons of the group of Dr. Ort for offering a place in
their lab for the microinjections of the viral vector.

A special thank goes to all the persons in the different animal houses for the care they
gave to all the ‘small-cute-rats’…

I would also like to thank all my collaborators of the ex-LN2C in Strasbourg:
Alexandra(s), Alex, Anne, Brigitte, Catherine, Chantal, Christophe, Ekatarina, Erwan,
Hélène, Karin, Monique, Olivier, Patrick, Rodrigue, Sarah... I really enjoyed the time
spent during my DEA and my PhD. I especially thank Sami Ben Hamida for his
participation to the last experiment of this work, and the great time we had in Freiburg
even during the very long “injections days”. My very special thank goes to Julie Koenig
with whom I started my work. Our discussions always had positive issues in scientific
or non-scientific life. Of course, the time in the lab wouldn’t have been the same without
our laughing and relaxing moments…I wish you the very best for your future life, a lot
of success in science and a lot of happiness in your personal life!

I don’t forget my "lab girls" from Freiburg, Anja, Sanni, Katha and Franzi. I really
enjoyed our every-day life in the lab and all the moments spent there. The four years of
my time in Freiburg wouldn’t have been the same without our discussions, jokes and
ice creams!

Je tiens également à remercier mes amis, ceux qui, en dehors du laboratoire m’ont
soutenu dans les moments difficiles et m’ont permis de garder un équilibre
indispensable. Merci tout particulièrement à Julie & David, Oliv, Dricky, Cris, Lolotte,
Pacal !

Je n’oublie pas mes parents, sans qui je ne serai pas là aujourd’hui. Leur soutien de
tous les moments m’a été indispensable pour atteindre mon but. Je ne vous
remercierai jamais assez de tout ce que vous avez fait pour moi ! Ma petite sœur
également, Camille à qui je souhaite tout le courage nécessaire pour la fin de ses
études !! Je te souhaite de trouver ta voie et d’être heureuse.
Antoine, ton soutien quotidien, ta patience, ta motivation aussi, m’ont sans aucun doute
permis d’achever ce travail. J’ai hâte de vivre "la vraie vie", celle qui nous attend, avec
toi !

II EUROPEAN DOCTORAL COLLEGE
EEuurrooppeeaann DDooccttoorraall CCoolllleeggee:: EEuurrooppeeaann DDooccttoorraall CCoolllleeggee::

« Melle RIEGERT Céline a été membre de la promotion Copernic du Collège Doctoral
Européen des universités de Strasbourg pendant la préparation de sa thèse de 2003 à
2007. Elle a bénéficié des aides spécifiques du CDE et a suivi un enseignement
hebdomadaire sur les affaires européennes dispensé par des spécialistes
internationaux.
Ses travaux de recherche ont été effectués dans le cadre d’une convention de cotutelle
avec l’université Albert Ludwigs de Freiburg (Allemagne) et l’Université Louis Pasteur
de Strasbourg (France) ».


« Ms RIEGERT Céline was a member of the European Doctoral College of the
Universities of Strasbourg during the preparation of her PhD, from 2003 to 2007, class
name Copernic. She has benefited from specific financial supports offered by the
College and, along with her mainstream research, has followed a special course on
topics of general European interests presented by international experts. This PhD
research project has been led with the collaboration of two universities: the University
Albert Ludwigs of Freiburg (Germany) and the Louis Pasteur University in Strasbourg
(France). »


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