Biology Scope approved at audit 2 Aug, 09

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• Publié par: Emme
• Nombre de lectures: 138
• Langue: English

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NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
SCOPE OF ACCREDITATION - MICROBIOLOGY


SL GROUP OF SPECIFIC TESTS OR TYPES OF SPECIFICATION, STANDARD ( RANGE OF TESTING/ MU
NO. PRODUCTS, TESTS PERFORMED METHOD) OR TECHNIQUE USED LIMIT OF DETECTION (±)
Antilog MATERIALS OR
ITEMS TESTED
1 WATER E. coli/250ml IS 15185 : 2002 Absent/Present NA
(PACKAGED Coliforms/250ml NA
DRINKING
WATER Faecal streptococci/250ml IS 5887 (part2): 1976 NA
OTHER 0.3MPN
THAN Staphylococcus Absent/Present NA
NATURAL aureus/250ml
MINERAL Pseudomonas IS: 13428 ANNEX D.1998 NA
WATER aeruginosa /250ml
IS Sulphite reducing bacteria IS:13428 ANNEX C.1998 Absent/Present NA
14543:2004 /50ml
PACKAGED Aerobic microbial IS 5402 :2002 1 cfu/ml 0.0742
NATURAL 0.0299 count/ml at 22? C & 37?C
MINERAL Yeast & Mould/250ml IS: 5403.1999 Absent/Present NA
WATER IS Salmonella/250ml IS 15187 (part 3):1999 NA
13428:1998 Shigella/250ml IS 5887 ( part 7):1999 NA
DRINKING Vibrio cholerae/250ml IS: 5887 (PART V) NA
WATER Vibrio IS 5887 (part 5) :1976 Absent/Present NA
IS parahaemolyticus/250ml
10500:1991

PROCESS Coliform bacteria IS 1622: 1981: reaffirmed MPN =1/100ml NA
WATER in 1996
IS Standard plate count 1 cfu/ml 0.0299
4251:1967 in 1996
98/83/EC Proteolytic bacteria IS 4251: 1967 Appendix A 5 cfu/ml NA
Lipolytic bacteria 5 cfu/ml NA
stE. coli/100 ml APHA:21 Ed 9221C, F 1 cfu 0.044
MPN =1.8 - 1600
Page 1 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR TYPES OF SPECIFICATION, SL RANGE OF TESTING/ MU
PRODUCTS, TESTS PERFORMED STANDARD ( METHOD) OR NO. LIMIT OF DETECTION (±)Antilog
MATERIALS TECHNIQUE USED
OR ITEMS
TESTED
1 WATER Faecal streptococci APHA:21st Ed 9230B MPN <1.8 - 1600/100ml NA
Salmonella APHA: 21st Ed 9260B Absent/Present NA
Shigella d 9260E NA
Vibrio cholerae/100 ml d 9260H NA
Pseudomonas aeruginosa APHA: 21st Ed 9213F MPN=1.8 to 1600/100 ml NA
EN 12780:2002 Absent/Present in 250 ml
Heterotrophic plate count d 9215B 1cfu/ml or 1 cfu/100 ml NA
Total coliforms/100 ml APHA: 21st Ed 9221B MPN =1.8 to 1600 NA
Faecal coliforms/100ml d 9221E =1.8 NA
Total Plate Count ISO 6222(1999e) 1 cfu 0.0742
0 0at 22 C & 37 C/ml 0.0299
Enterococci/100 ml IS 15186: 2002 Absent / Present NA
Clostridium perfringens Annex III 98/83 EC Absent /Present NA
including spores/100 ml

Page 2 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( METHOD) OR SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS TECHNIQUE USED NO. LIMIT OF DETECTION (±)
MATERIALS PERFORMED Antilog
OR ITEMS
TESTED
TH2. MARINE Total plate count/G IS : 5402 – 2002, BAM 8 Ed Ch 3 Jan = 10 cfu per g 0.03
PRODUCTS 2001, AOAC Petrifilm method
RAW Total coliforms/G BAM 8th Ed Ch-4 Sep 2002 IS 5401 = 10 cfu 0.0626
/FROZEN Part 1 & 2, 2002, Petrifilm method MPN =3 to 1100
SQUID =0.3
CUTTLEFISH E. coli/G BAM 8th Ed Ch-4 Sep 2002 – IS 5887 = 10 cfu 0.0905
OCTOPUS (Part 1) 1976, Reaffirmed 1995, AOAC MPN =3 to 1100
FISH Petrifilm method =0.3 to 110
SHRIMPS Staphylococcus BAM 8th Ed Ch-12 Jan 2001, AOAC = 10 cfu 0.028
SQUID aureus/G Petrifilm Method, IS: 5887 (PART II) MPN =3 to 1100
TUBES 1976 =0.3 to 110
TENTACLES Faecal IS: 5887 (PART II) 1976 10 cfu 0.398
AND CRAB streptococci/G MPN =0.3 to 110
MEAT Clostridium BAM 8th Ed Ch-16 Jan 2001 = 10 cfu per g PENDING
perfringens
Salmonella BAM 8th Ed Ch-5 Apr 2003, IS 5887( Absent/Present in 25g NA
Part 3 ) 1999,
Shigella BAM 8th Ed Ch - 6 Jan 2001, NA
IS 5887 ( Part 7 ) 1999
Vibrio cholerae BAM:8th Ed Ch – 9 May 2004 IS 5887 ( NA
Part 5 ) 1976 Reaff 1995
Vibrio BAM 8th Ed Ch-9 May 2004 Absent/Present in 25g NA
parahaemolyticus
Vibrio vulnificus d Ch- NA
Bacillus cereus/G BAM 8th Ed Ch-14 Jan 2001 = 10 cfu 0.0108
MPN =3 to >1100
Listeria d Ch-10 Jan 2003 Absent/Present in 25g NA
monocytogenes Petrifilm Method, ISO 11290-2 : 1998 = 10 cfu per g
thThermo tolerant BAM 8 Ed Ch –4 , Sep 2002 NA
coliforms/ F. MPN = 3 – 1100/g
coliforms
Enterobacteriacea IS 7402:1993, Petrifilm Method = 10 cfu 0.22
MPN = 0.3 – 110/g
thYeast & Mould/G IS: 5403.1999 BAM 8 Ed Ch 18, AOAC 10 cfu NA
Petrifilm Method 100 cfu
Page 3 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005


GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS METHOD) OR TECHNIQUE USED SL LIMIT OF DETECTION (±)Antilog
MATERIALS OR PERFORMED NO.
ITEMS TESTED
TH3. RAW/ Total plate count IS : 5402 – 2002, BAM 8 = 10cfu 0.03
FROZEN /G Ed Ch – 3 Jan 2001 AOAC
MEAT Petrifilm method
0.0626 PRODUCTS Total coliforms/G BAM 8th Ed Ch- 4 = 10 cfu
COOKED Sept: 2002, IS 5401 Part MPN =3 to 1100
SHRIMPS 1&2: 2002, AOAC Petrifilm =0.3 to 110 method
SQUID 0.0905 E. coli/G BAM 8th Ed Ch - 4 =10 cfu
RINGS Sept: 2002 IS 5887 MPN =3 to 1100
& (Part 1 ) 1976, Reaffirmed =0.3 to 110
TENTACLES 1995, AOAC Petrifilm
method
thThermo tolerant BAM 8 Ed Ch –4 , Sep : NA
coliforms /G 2002 MPN =3 to 1100
thF. coliforms BAM 8 Ed Ch –4 , Sep : NA
2002 MPN =3 to 1100
0.22 Enterobacteriacea IS 7402:1993, AOAC Perifilm =10 cfu
Method
thYeast & IS: 5403.1999, BAM 8 Ed =10 cfu, PENDING
Mould/G Ch 18, AOAC Petrifilm 100 cfu
Method
0.028 Staphylococcus BAM 8th Ed Ch - 12 =10 cfu per g
aureus /G Jan:2001, AOAC Petrifilm MPN <3 to 1100
Method
Clostridium BAM 8th Ed Ch-16 Jan 2001 =10 cfu PENDING
perfringens/G
0.398 Faecal IS: 5887 (PART II) 1976 =10 cfu
streptococci/G MPN =0.3 TO 110
Salmonella BAM 8th Ed Ch - 5 Apr Absent/Present in NA
2003 25g
Page 4 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR SPECIFICATION, SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS STANDARD ( METHOD) NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR ITEMS PERFORMED OR TECHNIQUE USED
TESTED
Shigella BAM 8th Ed Ch - 6 Absent/Present in 25g NA
Jan: 2001
Vibrio cholerae BAM 8th Ed Ch-9 NA
May 2004
Vibrio BAM 8th Ed Ch-9 NA
parahaemolyticus
Vibrio vulnificus BAM 8th Ed Ch-9 Absent/Present in 25g NA
May 2004
Bacillus cereus/G BAM 8th Ed Ch- =10 cfu .0108
14 Jan 2001 MPN =3 to 1100

Listeria BAM 8th Ed Ch-10 Absent/Present in 25g NA
monocytogenes Jan 2003, Petrifilm =10 cfu per g
Method
ISO 11290-2 :1998
Page 5 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS METHOD) OR TECHNIQUE USED NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR PERFORMED
ITEMS TESTED
TH4. BLACK Total plate count IS : 5402, 2002, BAM 8 Ed = 10cfu per g 0.477
PEPPER, /G Ch– 3 Jan 2001 AOAC
PROCESSED Petrifilm method
FOOD Enterobacteriaceae IS 7402:1993, AOAC Petrifilm = 10cfu 1.274
CONTAINING /G Method MPN =0.3 to 110
thRED PEPPER Yeast & Mould/G IS: 5403.1999 BAM 8 Ed Ch =10 cfu, =100 cfu 1.017
AND OTHER 18, AOAC Petrifilm Method
SPICES AND Total coliforms/G BAM 8th Ed Ch-4 Sep 2002, = 10 cfu per g 1.274
CONDIMENTS AOAC Petrifilm Method MPN <3 to 1100
E. coli/G d Ch-4 Sep 2002, =10 cfu 1.274
MPN =3 to 1100
Staphylococcus BAM 8th Ed Ch-12 Jan =10 cfu PENDING
aureus /G 2001, AOAC Petrifilm Method MPN =3 to 1100
Clostridium BAM 8th Ed Ch-16 Jan:2001 =10 cfu per g PENDING
perfringens
Faecal IS: 5887 (PART II) 1976 =10cfu
streptococci/G =0.3 MPN to 1100

Salmonella BAM 8TH Ed.Ch- 5 April Absent/Present in 25g NA
2003
Shigella BAM 8th Ed Ch-6 Jan: 2001 NA
Bacillus cereus d Ch-10 Jan 2003 NA

thListeria BAM 8 Ed Ch-14 Jan 2001, Absent/Present in 25g, NA
monocytogenes AOAC Petrifilm Method =10 cfu/g
ISO 11290-2 : 1998
Lactic acid bacteria IS 12899:1989 =10 cfu per g PENDING



Page 6 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
GROUP OF SPECIFIC TESTS OR TYPES SPECIFICATION, STANDARD ( SL RANGE OF TESTING/ MU
PRODUCTS, OF TESTS PERFORMED METHOD) OR TECHNIQUE USED NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR
ITEMS TESTED
TH5 MILK & MILK Total plate count/G IS : 5402, 2002, BAM 8 =10 cfu 0.266
POWDER Ed Ch–3 Jan 2001 AOAC
Petrifilm method
PENDING Total coliforms/G IS 5401 ( Part 1) 2002, IS =10 cfu
5401 Part 1 2002, BAM MPN =3 to 1100
th8 Ed Ch 4 Sep 2002, =0.3 to 110
AOAC Petrifilm Method
th PENDING Yeast & Mould/G IS: 5403.1999, BAM 8 =10 cfu
Ed Ch 18, AOAC Petrifilm =100 cfu
Method
PENDING E. coli/G IS 5887 ( P