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Publié par | ludwig-maximilians-universitat_munchen |
Publié le | 01 janvier 2011 |
Nombre de lectures | 55 |
Langue | Deutsch |
Poids de l'ouvrage | 26 Mo |
Extrait
Dissertation zur Erlangung des Doktorgrades der Fakultät fur Chemie und
Pharmazie der Ludwig-Maximilians-Universität München
5.5 Å Structure of the Eukaryotic
Ribosome
Jean-Paul Armache
aus Łódź, Polen
2011Erklärung
Diese Dissertation wurde im Sinne von § 13 Abs. 3 bzw. 4 der Promotionsordnung vom 29.
Januar 1998 von Herrn Prof. Dr. Roland Beckmann betreut.
Ehrenwörtliche Versicherung
Diese Dissertation wurde selbstständig, ohne unerlaubte Hilfe erarbeitet.
München, am 25.02.2011
Jean-Paul Armache
Dissertation eingereicht am: 25.02.2011
1. Gutachter: Herr Prof. Dr. Roland Beckmann
2. Gutachter: Herr Prof. Dr. Karl-Peter Hopfner
Mündliche Prüfung am: 04.05.2011 Table of Contents
Acgedwtemelosknn ................................................................................................................6
Summary ................................................................................................................................8
1 I........................9
1.1 Th F G Ia ...................................................................................9
1.2 R s ..................................................10
1.3 Ea R .................................................................................................11
1.4 R B...11
1.5 Traa Ma ...............................................................................................12
1.5 P -‐bod n Fmaro tnoi.............................14
1.6 Ra .....................................................................................................15
1.7 S Ea R -‐ I ......................................................16
1.8 A (O T) ..........................................................16
2 Maa a M.....................................................................18
2.1 Sa Paa .....................................18
2.1.1 Sa Paa ( Ha e a., 2004) ............................18
2.1.2 Sac mchesyaor aeisicevr Knockout Strains Sample Preparatio..........................n 19
2.1.3 Aaa R Sa Paa ( M a., 2010) ..................20
2.2 C -‐Elotec rn Mypcsooicr ...........................................................................................21
2.2.1 Sa Paa..............................22
2.2.2 Ia Ga .................................................................................................22
2.2.3 Ia Prescgonsi..23
2.2.4 R Da .....................................................................................25
2.2.5 Ma D..............................25
2.3 M .....................................................................................................................25
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2.3.1 P Tea S ................................................................................26
2.3.2 Caa/H M........26
2.3.3 Sa S P ............................................................................27
2.3.4 D -‐bad M h Snda S P ..............................28
2.3.5 R B F ..................................................................................................29
2.3.6 Ma Da F F......29
2.3.6 M W Sa ..............................................................................29
2.4 F ........................................................31
3 R...............................................................................................32
3.1 A C -‐EM Ma a Ea R a 5.5 ......................32
3.1.1 D .................................................................................................................33
3.1.2 Fa ................................................................................................33
3.2 M o D...........................39
3.2.1 C C ....................................................................................................39
3.2.2 Ua Eaa -‐Spciecif Psnteoir...42
3.2.3 Ea -‐Specf cii P rnteoi Esxntoseni ..................................................................47
3.3 Protein Localization .....................................53
3.3.1 Sa S P..........................................................54
3.3.1.3 Sa S B ................................................................58
3.3.2 Large Subunit Proteins..........................................................58
3.3.3 Th R maiednr .....................................................................68
4 C a D...............................................................70
4.1 C -‐Elotec rn Mypcs ooicr Map fo ..................70
4.1.1 Fa V a G R ....................................................................70
4.1.2 I O E R Ea R ................70
4.1.3 C -‐EM a aa a ...........................................71
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4.2 M .........................................................................................................................72
4.2.1 Loc alzati o in fo et h Nv oel Pteoisrn .........................................................................72
4.2.2 R P ...................................74
4.2.3 I F a Ia .........................................................................83
4.2.4 P .....................85
4.2.5 Ra M .........................................................................................88
4.2.6 Caa Aa Ca S.......................90
4.3 R L E ..............................................................................94
5 Sa ...........................................................98
6 A.........................................................................................100
7 C a ..............................................127
8 R.......................................................................................129
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odleAcknowledgements
I would like to thank my supervisor, Prof. Roland Beckmann for allowing me to conduct
research in his group in the Gene Center. I am grateful for his trust in a computer scientist
working in Structural Biology, for the unique working conditions, atmosphere and his
constant support.
Many thanks to Dr. Daniel Wilson for his patience, availability, openness to discussions and
the immeasurable amount of time and work he invested in this project.
I wish to express a deep sense of gratitude to a number of people I had pleasure of working
with. Dr. Thomas Becker, who taught me everything I know about cryo-electron microscopy
and always had time to help me, no matter how busy he was; Dr. Shashi Bhushan, for the
fruitful collaboration and the tremendous amount of high-quality data no one else could have
acquired; Andreas Anger, Alexander Jarasch and Elizabeth Villa for all the time spent
together, the time