Characterization of Cellular DNA Repair Capacity by Molecular and Cytogenetic Approaches: Suitability as Intermediate Phenotype of Cancer [Elektronische Ressource] / Harald Martin Surowy

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INSTITUTE OF H UMAN GENETICS OF THE U NIVERSITYH OSPITAL OF U LM MEDICAL DIRECTOR: PROF DR. C. KUBISCH CHARACTERIZATION OF CELLULAR DNA REPAIR CAPACITY BY MOLECULAR AND CYTOGENETICA PPROACHES : SUITABILITY ASI NTERMEDIATEP HENOTYPE OFC ANCER DISSERTATION FOR THE DOCTORAL DEGREE OF H UMAN B IOLOGY (DR. B IOL. H UM .) FACULTY OF M EDICINE , U LM U NIVERSITY SUBMITTED B Y H ARALD M ARTIN SUROWY B ORN IN M EMMINGEN , GERMANY 2011 Acting Dean: Prof. Dr. Thomas Wirth 1. Correspondent: Prof. Dr. Walther Vogel 2. Correspondent: PD Dr. Marcus Cronauer stDay of Promotion: October 21, 2011 TABLE OF C ONTENTS PAGE i Table of Contents LIST OFA BBREVIATIONS II I1 INTRODUCTION 1 1.1 Genetic Predisposition to Cancer 1 1.1.1 Cancer Heritability and Susceptibility 1 1.1.2 DNA Repair Disorders and Cancer Risk 3 1.2 Cellular DNA Repair Capacity and Cancer 6 1.3 Assays Used for the Measure of Cellular DNA RepaCiarp acity from Peripheral Blood Samples 7 1.3.1 Micronucleus Assay 7 1.3.2 Sister Chromatid Exchange Assay 8 1.3.3 Mitotic Delay Assay 10 1.3.4 Further Measures of DNA Repair Capacity 10 1.4 Aims of the Study 11 2 PROBANDS , MATERIALS AND METHODS 13 2.1 Probands 13 2.1.1 Cohorts with DNA Repair and Genotype Data 13 2.1.2 Cohorts with Genotype Data Only 14 2.2 Materials 16 2.2.1 Sources of Peripheral Blood Lymphocytes andN AD 16 2.2.

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• Publié par: universitat_ulm
• Publié le: 01 janvier 2011
• Nombre de lectures: 20
• Langue: English
• Poids de l'ouvrage: 2 Mo

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