Chromosome distribution in human sperm – a 3D multicolor banding-study

Manvelyan Marina, Hunstig Friederike, Bhatt Samarth, Mrasek Kristin, Pellestor Franck, Weise Anja, Simonyan Isabella, Aroutiounian Rouben, Liehr, Liehr Thomas

Description

Nuclear architecture studies in human sperm are sparse. By now performed ones were practically all done on flattened nuclei. Thus, studies close at the in vivo state of sperm, i.e. on three-dimensionally conserved interphase cells, are lacking by now. Only the position of 14 chromosomes in human sperm was studied. Results Here for the first time a combination of multicolor banding (MCB) and three-dimensional analysis of interphase cells was used to characterize the position and orientation of all human chromosomes in sperm cells of a healthy donor. The interphase nuclei of human sperm are organized in a non-random way, driven by the gene density and chromosome size. Conclusion Here we present the first comprehensive results on the nuclear architecture of normal human sperm. Future studies in this tissue type, e.g. also in male patients with unexplained fertility problems, may characterize yet unknown mechanisms of infertility.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2008
• Nombre de lectures: 6
• Langue: English
• Poids de l'ouvrage: 1 Mo

Extrait

Molecular Cytogenetics

BioMedCentral

Open Access Research Chromosome distribution in human sperm – a 3D multicolor bandingstudy 1,2,3 3 3,4,5,6 Marina Manvelyan* , Friederike Hunstig , Samarth Bhatt , 3 4,5,7 3 2 Kristin Mrasek , Franck Pellestor , Anja Weise , Isabella Simonyan , 1 3 Rouben Aroutiounian and Thomas Liehr

1 2 Address: Department of Genetic and Laboratory of Cytogenetics, State University, Yerevan, Armenia, Research Centre of Maternal and Child 3 4 Health Protection, Yerevan, Armenia, Institute of Human Genetics and Anthropology, Jena, Germany, INSERM U847, Montpellier, France, 5 6 7 University of Montpellier I, Montpellier, France, Baylor College of Medicine, Houston, Texas, USA and Department of Reproduction biology, CHU Montpellier, Montpellier, France Email: Marina Manvelyan*  [e-mail]; Friederike Hunstig  [e-mail]jena.de; Samarth Bhatt  [e-mail]; Kristin Mrasek  [e-mail]jena.de; Franck Pellestor  fpellestor@chumontpellier.fr; Anja Weise  [e-mail]jena.de; Isabella Simonyan  [e-mail]; Rouben Aroutiounian  [e-mail]; Thomas Liehr  [e-mail]jena.de * Corresponding author

Published: 14 November 2008 Received: 23 September 2008 Accepted: 14 November 2008 Molecular Cytogenetics2008,1:25 doi:10.1186/17558166125 This article is available from: http://www.molecularcytogenetics.org/content/1/1/25 © 2008 Manvelyan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Nuclear architecture studies in human sperm are sparse. By now performed ones were practically all done on flattened nuclei. Thus, studies close at thein vivostate of sperm, i.e. on threedimensionally conserved interphase cells, are lacking by now. Only the position of 14 chromosomes in human sperm was studied.

Results:Here for the first time a combination of multicolor banding (MCB) and threedimensional analysis of interphase cells was used to characterize the position and orientation of all human chromosomes in sperm cells of a healthy donor. The interphase nuclei of human sperm are organized in a nonrandom way, driven by the gene density and chromosome size.

Conclusion:Here we present the first comprehensive results on the nuclear architecture of normal human sperm. Future studies in this tissue type, e.g. also in male patients with unexplained fertility problems, may characterize yet unknown mechanisms of infertility.

Background Interphase chromosome organization and nuclear archi tecture are already being investigated for a long time [13]. Chromosomes have been demonstrated to be located in specific regions in the interphase nucleus. These were called 'chromosome territories' [47]. However, our own multicolor banding (MCB) based studies [8] showed, that the chromosome shape is not lost in the interphase nucleus and one can even identify interphase chromo

somes instead of only chromosome territory [911]. MCB is the only approach available at present that provides the possibility of characterizing the chromosomal integrity of arbitrary interphase cell populations [12,13]. It is still a matter of discussion what influences more the nuclear position of chromosomes: chromosome size or gene den sity. It has been repeatedly shown that small chromo somes preferentially locate close to the center of the nucleus, while large chromosomes can be found in the

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