Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

Tai Cheng-Jeng, Su Tzu-Cheng, Jiang Ming-Chung, Chen Hung-Chang, Shen Shing-Chuan, Lee Woan-Ruoh, Liao Ching-Fong, Chen Ying-Chun, Lin Shu-Hui, Li Li-Tzu, Shen Ko-Hung, Yeh Chung-Min, Yeh Kun-Tu, Lee Ching-Hsiao, Shih Hsin-Yi, Chang Chun-Chao

Description

Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer. Methods The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed. Results CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage ( P =0.003) and depth of tumor penetration ( P =0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis Conclusions CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

Sujets

• Colorectal cancer
• Cytoplasm
• Invasión
• Metástasis
• Nucléus

Informations

• Publié par: biomed
• Publié le: 01 janvier 2013
• Nombre de lectures: 10
• Langue: English
• Poids de l'ouvrage: 2 Mo

Extrait

Taiet al. Journal of Translational Medicine2013,11:29 http://www.translationalmedicine.com/content/11/1/29

R E S E A R C HOpen Access Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage 1,2†3,5†1 4 6 ChengJeng Tai, TzuCheng Su, MingChung Jiang , HungChang Chen , ShingChuan Shen , 6 7 73,5 73 WoanRuoh Lee , ChingFong Liao , YingChun Chen , ShuHui Lin, LiTzu Li , KoHung Shen , 3,5 3,8,95 71,10* ChungMin Yeh, KunTu Yeh, ChingHsiao Lee , HsinYi Shihand ChunChao Chang

Abstract Background:Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer. Methods:The invasion and metastasisstimulating activities of CSE1L were studied byin vitroinvasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed. Results:CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Nonneoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis Conclusions:CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis. Keywords:Colorectal cancer, CSE1L, Cytoplasm, Invasion, Metastasis, Nucleus

Background Colorectal cancer (CRC) shows a high rate of metasta sis and is one of the most lethal cancers worldwide. Metastasis, especially occult metastasis, contributes considerably to the challenge of defining the prognosis for a patient with this disease. Approximately 60% of patients who undergo curative resection experience

* Correspondence: [e-mail] † Equal contributors 1 Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No.250, WuHsing St., Taipei 11031, Taiwan 10 Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, No.250, WuHsing St., Taipei 11031, Taiwan Full list of author information is available at the end of the article

local recurrence or distant metastases [1]. The primary treatment of CRC is surgical resection of the primary tumor and possibly the regional lymph nodes; this may be combined with chemotherapy, depending on the depth of tumor penetration and the disease stage [2]. The development and progression of a tumor is con trolled by oncogenes and tumor suppressor genes. Alterations in the expression of any of these genes in a tumor may correlate with the clinicalpathological characteristics and behavior of CRC. Thus, examin ation of these gene expressions may be useful for prognosis of the disease. Chromosome segregation 1like protein (CSE1L) is the human homologue of CSE1, the yeast chromosome seg regation protein [3]. CSE1L is highly expressed in most

© 2013 Tai et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.