Descending serotonergic facilitation and the antinociceptive effects of pregabalin in a rat model of osteoarthritic pain

Descending serotonergic facilitation and the antinociceptive effects of pregabalin in a rat model of osteoarthritic pain

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Descending facilitation, from the brainstem, promotes spinal neuronal hyperexcitability and behavioural hypersensitivity in many chronic pain states. We have previously demonstrated enhanced descending facilitation onto dorsal horn neurones in a neuropathic pain model, and shown this to enable the analgesic effectiveness of gabapentin. Here we have tested if this hypothesis applies to other pain states by using a combination of approaches in a rat model of osteoarthritis (OA) to ascertain if 1) a role for descending 5HT mediated facilitation exists, and 2) if pregabalin (a newer analogue of gabapentin) is an effective antinociceptive agent in this model. Further, quantitative-PCR experiments were undertaken to analyse the α 2 δ-1 and 5-HT3A subunit mRNA levels in L3–6 DRG in order to assess whether changes in these molecular substrates have a bearing on the pharmacological effects of ondansetron and pregabalin in OA. Results Osteoarthritis was induced via intra-articular injection of monosodium iodoacetate (MIA) into the knee joint. Control animals were injected with 0.9% saline. Two weeks later in vivo electrophysiology was performed, comparing the effects of spinal ondansetron (10–100 μg/50 μl) or systemic pregabalin (0.3 – 10 mg/kg) on evoked responses of dorsal horn neurones to electrical, mechanical and thermal stimuli in MIA or control rats. In MIA rats, ondansetron significantly inhibited the evoked responses to both innocuous and noxious natural evoked neuronal responses, whereas only inhibition of noxious evoked responses was seen in controls. Pregabalin significantly inhibited neuronal responses in the MIA rats only; this effect was blocked by a pre-administration of spinal ondansetron. Analysis of α 2 δ-1 and 5-HT3A subunit mRNA levels in L3–6 DRG revealed a significant increase in α 2 δ-1 levels in ipsilateral L3&4 DRG in MIA rats. 5-HT3A subunit mRNA levels were unchanged. Conclusion These data suggest descending serotonergic facilitation plays a role in mediating the brush and innocuous mechanical punctate evoked neuronal responses in MIA rats, suggesting an adaptive change in the excitatory serotonergic drive modulating low threshold evoked neuronal responses in MIA-induced OA pain. This alteration in excitatory serotonergic drive, alongside an increase in α 2 δ-1 mRNA levels, may underlie pregabalin's state dependent effects in this model of chronic pain.

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Ajouté le 01 janvier 2009
Nombre de lectures 700
Langue English
Signaler un abus
aPeg1 f  o(p17e agmbnun ref toc rotatipurpion )oses
Abstract Background:Descending facilitation, from the br ainstem, promotes spinal neuronal hyperexcitability and behavioural hypersensitivity in many chronic pain states. We have previously demonstrated enhanced descending facilitation onto dorsal horn neurones in a neuropathic pain model, and shown this to enable the analgesic effe ctiveness of gabapentin. Here we have tested if this hypothesis applies to other pain states by using a combination of approaches in a rat model of osteoarthritis (OA) to as certain if 1) a role for descending 5H T mediated facilitation exists, and 2) if pregabalin (a newer analogue of gabapentin) is an effective antinociceptive agent in this model. Further, quantitative-PCR experiment s were undertaken to analyse the α 2 δ -1 and 5-HT3A subunit mRNA levels in L3–6 DRG in order to assess wh ether changes in these mo lecular substrates have a bearing on the pharmacological effects of ondansetron and pregabalin in OA. Results: Osteoarthritis was induced via intra-articula r injection of monosodium iodoacetate (MIA) into the knee joint. Control animals were injected with 0.9% saline. Two weeks later in vivo electrophysiology was performe d, comparing the effects of spinal ondansetron (10–100 μ g/50 μ l) or systemic pregabalin (0.3 – 10 mg /kg) on evoked responses of dors al horn neurones to electrical, mechanical and thermal stimuli in MIA or contro l rats. In MIA rats, ondansetron significantly inhibited the evoked responses to both innocuous and noxious natural evoked neuronal responses, whereas only inhibition of noxious evoked response s was seen in controls. Pregabalin significantly inhibited neuronal responses in the MIA rats only ; this effect was blocked by a pre-administration of spinal ondansetron. Analysis of α 2 δ -1 and 5-HT3A subunit mRNA levels in L3–6 DRG revealed a significant increase in α 2 δ -1 levels in ipsilateral L3&4 DRG in MIA rats. 5-HT3A subunit mRNA levels were unchanged. Conclusion: These data suggest descending serotonergic facilitation pl ays a role in mediating the brush and innocuous mechanical punctate evoked neuronal responses in MIA rats, suggesting an adaptive change in the excitato ry serotonergic drive modulating low threshold evoked neuronal responses in MIA-induced OA pain. This alteration in excitatory serotone rgic drive, alongside an increase in α 2 δ -1 mRNA levels, may underlie pregabalin's state dependent effects in this model of chronic pain.
Published: 7 August 2009 Received: 6 April 2009 Molecular Pain 2009, 5 :45 doi:10.1186/1744-8069-5-45 Accepted: 7 August 2009 This article is available from: http ://www.molecularpain.com/content/5/1/45 © 2009 Rahman et al; licen see BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons. org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the orig inal work is properly cited.
Molecular Pain
Address: Department of Neuroscience, Phar macology and Physiology, University College London, Gower Street, London, WC1E 6BT, UK Email: Wahida Rahman* - w.rahman@ucl.ac.uk; Claudia S Bauer - c.bauer@ucl.ac.uk; Kirsty Ba nnister - kirsty.bannister@ucl.ac.uk; Jean-Laurent Vonsy - j.vonsy@ucl.ac.uk; Annette C Dolphin - a.dolphin@ucl.ac.uk; Anthony H Dickenson - anthony.dickenson@ucl.ac.uk Corresponding author *
Research Open Access Descending serotonergic facilit ation and the antinociceptive effects of pregabalin in a rat model of osteoarthritic pain Wahida Rahman*, Claudia S Bauer, Kirs ty Bannister, Jean-Laurent Vonsy, Annette C Dolphin and Anthony H Dickenson
Bio Med Central