Differential effect of DLX2 in neural precursors derived from the anterior and hippocampal subventricular zone [Elektronische Ressource] / presented by Yongjoon Suh
Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Diplom: Yongjoon Suh born in: Kangwondo, South Korea th Oral-examination: 12 Feb. 2009 Differential Effect of DLX2 in Neural Precursors Derived from the Anterior and Hippocampal Subventricular zone Referees Prof. Dr. Hilmar Bading Prof. Dr. Gabriele Elisabeth Pollerberg Table of Contents List of Figures 7 Summary 9 Zusammenfassung 10 Articles from this phD thesis 11 1. Introduction 12 1.1. Neurogenesis 1.2. Neural stem cells during embryonic development 13 1.3. Neural stem cells in the adult brain 16 1.4. Neurogenic regions in the postnatal brain 17 1.5. Regional specification and migration of neural precursors during embryonic development 21 1.6. Distal-less homeobox-2 (DLX2) 25 1.7. The Aims of the Work 28 2. Materials and Methods 29 2.1. 29 2.1.1. General reagents 29 2.1.2. Plasmids 30 2.1.3. Oligonucleotides 30 2.1.4. Enzymes 31 2.1.5. Quantitative PCR reagents 32 2.1.6. Mouse and cell lines 32 2.1.7. Cell culture reagents and media 32 2.1.8. Antibodies 34 2.1.8.1.