DNA hybrid materials consisting of oligonucleotides and organic molecules [Elektronische Ressource] : synthesis, characterization and applications / Diana Milena Anaya

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Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2010
• Nombre de lectures: 20
• Langue: English
• Poids de l'ouvrage: 16 Mo

Extrait

‘DNA Hybrid Materials Consisting of
Oligonucleotides and Organic Molecules:
Synthesis, Characterization and Applications’







Dissertation
zur Erlangung des Grades
“Doktor der Naturwissenchaften”




am Fachbereich
Chemie, Pharmazie und Geowissenschaften
der Johannes Gutenberg-Universität Mainz











Diana Milena Anaya
geb. in Bogotá, Kolumbien
Mainz 2010


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Dekan:
1. Berichterstatter:
2. Berichterstatter:
Tag der mündlichen Prüfung:



































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Dedicated to my parents























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“Science knows no country, because knowledge
belongs to humanity, and is the torch which
illuminates the world.”
(Louis Pasteur )













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9 TABLE OF CONTENTS


LIST OF ABBREVIATIONS ...............................................................................13

Chapter 1

INTRODUCTION ................................................................................................17

1.1 DNA fundamentals.......................................................................................18
1.2 DNA as a construction material for nanoarchitectures .................................25
1.3 DNA-based hybrid materials ........................................................................29
1.4 Summary......................................................................................................32
1.5 References...................................................................................................34
Chapter 2

MOTIVATION AND OBJECTIVES.....................................................................39

Chapter 3

AGGREGATION OF DOUBLE STRANDED DNA INDUCED BY AROMATIC
π-SYSTEMS.......................................................................................................43

3.1 Introduction ..................................................................................................43
3.2 Preparation of dsDNA-PDI hybrids ..............................................................44
3.3 Analysis on surface by Atomic Force Microscopy (AFM) .............................46
3.4 Native polyacrylamide gel electrophoresis (PAGE) Analysis .......................48
3.5 Fluorescence correlation spectroscopy (FCS) study of DNA-PDI hybrids in
.solution .........................................................................................................49
3.6 Photophysical study of DNA-PDI hybrids.....................................................52
3.7 Conclusions .................................................................................................55
3.8 Experimental Section ...................................................................................57
3.9 References...................................................................................................66
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