Effects of xenobiotics on the glutathione and glucose metabolism of cultured brain astrocytes [Elektronische Ressource] / vorgelegt von Maike M. Schmidt

Sujets

• Biologie

Informations

• Publié par: universitat_bremen
• Publié le: 01 janvier 2010
• Nombre de lectures: 28
• Langue: English
• Poids de l'ouvrage: 24 Mo

Extrait

Effects of Xenobiotics on the Glutathione and
Glucose Metabolism of Cultured Brain Astrocytes

Dissertation des Fachbereichs 2 Biologie/Chemie
Universität Bremen


zur Erlangung des Grades eines Doktors
der Naturwissenschaften


2010


vorgelegt von
Maike M. Schmidt























Tag der mündlichen Prüfung: 2. August 2010

Dekan: Prof. Dr. S. Kelm
1. Berichterstatter: Prof. Dr. R. Dringen (Universität Bremen)
2. Berichterstatter: Prof. Dr. S. Kelm (Universität Bremen)
3. Berichterstatter: Prof. Dr. J. P. Bolaños (Universität Salamanca, Spanien)

















This thesis is dedicated to my beloved family.



Content
I Acknowledgements I
II II Information on the structure of the thesis
III III Summary
IV IV Zusammenfassung
V V Abbreviations
1 Introduction 1
1.1 Properties and functions of astrocytes 3
1.2 Publication/Manuscript 1: 27
Schmidt, M. M. & Dringen, R. Glutathione synthesis and metabolism. In:
Advances in Neurobiology, Volume: Neural Metabolism in vivo (In-Young
Choi & Rolf Gruetter eds.). Springer Science, New York. In press.
2 Results 61
2.1 Astroglia-rich primary cultures as model system to study metabolism and 63
glutathione mediated detoxification of xenobiotics in brain astrocytes.
2.2 Publication 2: 115
Schmidt, M. M. & Dringen, R. (2009) Differential effects of
iodoacetamide and iodoacetate on glycolysis and glutathione
metabolism of cultured astrocytes. Front Neuroenergetics, 1, 1-10.
2.3 Publication 3: 127
Schmidt, M. M. & Dringen, R. (2010) Fumaric acid diesters deprive
cultured primary astrocytes rapidly of glutathione. Neurochem Int. In
press.
2.4 Publication/Manuscript 4: 137
Schmidt, M. M. et al.: Effects of chlorinated acetates on the glutathione
metabolism and on glycolysis of cultured astrocytes. Neurotox Res. In
revision.
2.5 Publication/Manuscript 5: 167
Schmidt, M. M. et al.: 2-Deoxyribose deprives cultured astrocytes of
their glutathione. Neurochem Res. Submitted.
3 Summarising Discussion 195
3.1 Effects of xenobiotics on the glutathione content 199
3.2 Effects of xenobiotics on the glucose metabolism 205
3.3 Future perspectives 206
3.4 References 209
4 Appendix 217
4.1 Curriculum vitae 219
4.2 List of publications 220





Acknowledgements


I Acknowledgements
Firstly I would like to thank Professor Ralf Dringen who offered me this doctoral
thesis and who supported me throughout this process of research and writing with
unswerving commitment. I am truly thankful for his unfailing accessibility,
constructive criticism, and encouragement. The numerous opportunities I was given
to attend national and international congresses were enriching experiences and will
undoubtedly help me to forge future professional plans. I am particularly thankful to
Professor Dringen for his faith in me to consider my opinion in some important
decisions.
I am very grateful to Professor Soerge Kelm for reviewing my thesis in addition to
his critical questions and inspiring comments throughout my work that proved to be
highly valuable.
Special thanks to Professor Juan Bolaños (University of Salamanca, Spain) for finding
the time to review my thesis on quite a short notice.
To all the members of Professor Dringen’s group who helped and guided me along
the way, who shared their scientific expertise with me and who, in some cases,
ultimately became friends, I extend my gratitude.
Excellent laboratory management is a prerequisite for good scientific work which
would not have been possible without Monica Cox and Yvonne Köhler. Many thanks
to both of them.
I would like to express sincere gratitude to Hendrik Koliwer-Brandl for the highly
efficient cooperation. Also special thanks to Astrid Rohwedder and Helena Greb for
their contribution to this thesis.
Sincere thanks to Peter Behrend (Centre for Environmental Research and
Sustainable Technology, University of Bremen) for establishing analytical
techniques to quantify glutathione conjugates and for giving me an insight into the
world of gas chromatography and mass spectrometry.
In the end I would like to thank my parents and my sister for their unconditional
love, support and never-ending patience.

I