Electron microscopic investigation of cytomegalovirus egress after high-pressure freezing, freeze-substitution and plastic embedding [Elektronische Ressource] / Christopher Buser
Z.E. Elektronenmikroskopie Prof. Dr. Paul Walther and Institut für Virologie Prof. Dr. Thomas Mertens Electron microscopic investigation of cytomegalovirus egress after high-pressure freezing, freeze-substitution and plastic embedding Dissertation zur Erlangung des Doktorgrades der Humanbiologie der Medizinischen Fakultät der Universität Ulm Christopher Buser, New York, N.Y., USA 2006 Amtierender Dekan: Prof. Dr. Klaus-Michael Debatin 1. Berichterstatter: Prof. Dr. Paul Walther 2. Berichterstatter: Prof. Dr. Thomas Mertens Tag der Promotion: 8. Dezember 2006 2Abstract Modern cryo-preparation techniques for biological electron microscopy have a great impact on the preservation of the sample structure. Among these, high-pressure freezing, freeze-substitution and plastic embedding of cell cultures for transmission electron microscopy allows new insights into various processes in cell biology due to its superiority over conventional preparation by chemical fixation. On the other side, the recent virological research on herpesviruses and especially on cytomegaloviruses has mainly relied on biochemistry, molecular biology and fluorescence microscopy techniques.