Experimental analysis of biaxial mechanical tension in cell monolayers and cultured three-dimensional tissues [Elektronische Ressource] : the celldrum technology / von Jürgen Trzewik

Jürgen Trzewik

Sujets

• Biologie
• Gesundheit

Informations

• Publié par: technische_universitat_ilmenau
• Publié le: 01 janvier 2008
• Nombre de lectures: 30
• Poids de l'ouvrage: 9 Mo

Extrait

Jürgen Trzewik

Experimental analysis of biaxial mechanical tension in
cell monolayers and cultured three-dimensional tissues



Experimental analysis of biaxial
mechanical tension in cell monolayers
and cultured three-dimensional tissues

- The CellDrum Technology -

Von Jürgen Trzewik
















Universitätsverlag Ilmenau
2008
Impressum


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über http://dnb.d-nb.de abrufbar.


Diese Arbeit hat der Fakultät Informatik und Automatisierung als Dissertation vorgelegen
Tag der Einreichung: 25. Oktober 2006
1. Gutachter: Prof. Dr.-Ing. habil. G. Henning
2. Gutachter: Prof. Dr.-Ing. Dr. med. H. Witte
3. Gutachter: Prof. Dr. rer. nat. habil. G.M. Artmann
Tag der Verteidigung: 23. November 2007


Technische Universität Ilmenau/Universitätsbibliothek
Universitätsverlag Ilmenau
Postfach 10 05 65
98684 Ilmenau
www.tu-ilmenau.de/universitaetsverlag


Herstellung und Auslieferung
Verlagshaus Monsenstein und Vannerdat OHG
Am Hawerkamp 31
48155 Münster
www.mv-verlag.de

ISBN 978-3-939473-26-8
urn:nbn:de:gbv:ilm1-2007000305

Meinen Eltern
1 Introduction and motivation 1
2 Mechanical structures in cells and tissues 2
2.1 Molecular and cellular level 2
2.2 Tissue and organ level 5
3 Experimental approaches to analyse the mechanical behaviours of tissues and organs 9
3.1 Tissue-stretch-technique 9
3.2 Sheet-wrinkling-technique 10
3.3 Microaspiration technique 11
3.4 Rectangular 3D-Gel and its variations 12
3.5 Uncommon approaches 13
3.6 Evaluation of cardiomyocytes 14
3.7 Summary of the current status 15
4 Objectives 17
4.1 Specification for a newly developed micromechanical experimental setup 17
5 The CellDrum principle 18
5.1 2D CellDrum 20
5.2 Fiber and mesh supported CellDrums for tissue application (3D) 20
6 Basic concepts 21
6.1 The Load-deflection concept 22
6.2 Steady state measurements 24
6.3 Dynamic measurements 24
7 Displacement Sensor concept 26
7.1 CCD Laser displacement Sensor 26
7.2 Sensor properties -custom made optical sensor 26
7.3 Software properties and function 28
7.4 Steady-state system: properties and limitations 35
7.5 Laser sensor dynamic system: Sensor properties 39
VII8 CellDrum technologies 42
8.1 Membrane fabrication technology 42
8.2 Biocompatibility 42
8.3 Force transduction between the silicon membrane and an individual cell? 46
8.4 Force transduction and surface coatings 52
8.5 Mechanical properties 55
9 Orientation of cells in a mechanically anisotropic environment 57
9.1 Effects of mechanical loading to tissue behaviour 57
9.2 Experimental setup 57
9.3 Microscopic analysis 58
9.4 Isotropicity and anisotropicity of cell distribution 59
10 Dynamic measurement of lateral tension in tissue equivalents 62
10.1 Modeling of rectangular and circular membranes 62
10.2 Cell orientation – isotropic/anisotropic behaviour 63
10.3 Theoretical considerations 64
10.4 Vibrational Modes of a Circular Membrane 64
10.5 Elastic membrane model 66
10.6 Experimental protocol: 69
10.7 Experimental results 70
10.8 Discussion 75
11 Steady state measurements: Self exciting myocardical cell layers 79
12 Residual stress and Elastic modulus of cell seeded thin film tissue constructs- a Load-deflection
measurement 86
12.1 Experimental setup 86
12.2 Data acquisition and fitting procedure 89
12.3 Evaluation study: Load-deflection relationship of pure CellDrum membranes 89
12.6 Mechanical properties of 3D tissue constructs 92
13 Summary and future prospects 97
VIII 14 Documentation 100
14.1 Good laboratory practice 100
14.2 Cell culture methods 101
14.2.1 Cell lines BAEC, 3T3 102
14.2.2 Tissue equivalent 102
14.2.3 Cultured heart tissue equivalent 103
14.2.4 Mito-capture™ Apotosis kit 104
14.2.5 Determination of F-actin 104
14.2.6 Fluorescence labeling of live cells for cell orientation studies 105
14.2.7 Cell-Tak™ 106
14.2.8 Protein profile analysis 106
15 Publications by J. Trzewik related to this and preceding work 108
16 Abstract 123
17 Kurzfassung 125


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