Fluctuation spectroscopy of single plasmonic nanostructures [Elektronische Ressource] : new perspectives due to improved time resolution / Irene Ament

Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2012
• Nombre de lectures: 21
• Langue: English
• Poids de l'ouvrage: 9 Mo

Extrait

FFluctuationIreneSpwissenschaftenectroscoprankfurtyPhaofGutenbSinglerenP2011lasmonicereichNanostructuresundNewderpUniversit?terspgebecntiMainveamsachbdueChemie,tormazieimpGeorovedantimeJohannesresolutionergDissertationMainzzurAmentErlangungodesiGradesFDoktoamrMainz,derNaturwissenschaftenPr?fungskDiesevArbErstereitersonalenItstand[pimGutacZeed]itraumJanM?rzv2008hbisdataNoZwvter:emremobagerquiums:20112012amremoInstituted]f?rGutacPhter:ysikersonalaliscremoheed]Chemie,eiterJohanneshGuten[pbdataergvUnivTersit?tdesMainz.olloDek10.an:uar[pIersonalIdataholarship.ImeaprocPhDknoforwledgevidingtheaCarlscZeissIVStiftung'tTVowrmIfywithfamilyPItardodoesne'teriment,matterRichowFbyouee.autifulityouresntheagroryeis,expitit'sdoong.esnhard't.mattereynmanhowsmartyAbstracttimesPlasmonicstudynanoparticlestsareaddressedgreat0.03candidatesminorforasensingesappunderl,icationsemenwithaop-tempticalIread-out.stPlasmontro-sensingbisdbasedFonhightheresonanceintimeteractionenoforts.theplananoparticlealterationwithSetup',electromagneticanwaayvtheesthewheretotheopparticofleofscatterssensorlighatvatditsbutresonanceiswelengthaanvise-factorlength.osureThisthanwunresolacvsensorelengthvironmendepmonitoredendstheonesevunlaberal.innamictrinsicevfactorsmelikineofmeatmoeelsrial,cshaptecetheandwsizengofecules.theisnanopartwimolecule,cleter-asIwcollapseelltheasxedextrinsicdiusingfathatctorsspliklesensingtheVIraeyfractivmeasuredexpindex10ofimprotheofsurrounding20medium.theTheislatterteallopreviouswsus,theablenanoparticleeletoofbmebusedcalasaabsensor;extremelycresolution.hangesFlincantheevprofximateproteinsennanorovironmendditionallytthecanolutionbproteinetdirectlytmThisonitoredofbrythethedomainswcanaedvstudyeleanalytesngthforofossiblytheconformationalemittedclightarget.t.alsoDueuctuationstovtheiramintousculervsizewnianandeledhighfurthersensitivitthisyusabilitthisnanoruler,allonanosphereswsaiandtheilinkvidualillumination.nanoparticlesvtotreptheortIonncofhangesdineledparticlethecoconcenvtoerage.uorescenceTctroscopoforuseAthiscalsinglenanoparticleparticlhieveItsplasmonwsensorvforstabilitfutofurenm,sewithnseiosurengofapms,planicationsvitthasatoofmeetevthethoughdemandexpfortimedetection3000ofshorincidenrtsinonreptheThsinglepreviouslymoleculevlevwel,vsucngthhhangesasthesinglesmoleculeonsensinginducedoryevloenenthetdetectionlofcanconformationalecwithhangeshighoforalaUsingsingle'Plasmonmolecule.uctuationTherefIorresolveadsorption,entiomesingleresolutioneledandonsensitivitindividualydhaAv,emonitoredtody-beveofenhancedsingleasbindingtoendaony'smillisecondmeasuremenitscale.methofeasibilitdsisforhighsignalteread-outeareastoroleocertainsloinwproteinandbnotprobsensitivbeaenoughoftodiedresolvwithouteneedtheselabpropceinsducingseors.haracteristicThishangesthesisthepresenThetshniquearesolvnewequilibriumeinxpcoerimenerage,taleningsetup,windothein'PlasmonoFluctuationseSetup',ithBroadynamicstunlableadsmacromoltoAtremendoustopicimproinvthesisementhetsyinthetimetresolutionoandconnectesensitivitwithyspacer.asThisstinessisforacinhievparticleederbstrongyHere,impldiscoemenertationlighofinducedaofstrongernanoruler.lighurthermore,texploitsourcesensiandgaolumemoreasensitivnanoroetodetector.unlabTheanalytesnewaroundsetupnanorohasatatrationstimeareresolutionoinforthecorrelationmicroseconderegime,yanrealisticadvbiologicaancemensystems.tdditionallyoflo4-6pHorderswithofsmagnitudeactoed.previousIsetups..Contents.1.Intro.duction.1.2.Plasmons.in.a.N.utshell4.15.2.1.Plasmonfosp2.6.2ectra..a.p.........................illuminated.Conclusion...32.concept...........ticle..............5.2.1.144Drude-Sommerfeld.mo.del........27.........b.............Nano.r.p..........7.2.1.2EnsembleQuasi-static.app.ro.ximation3.4.......3.........Plasmon.setup.......4.2.........erfo......9.2.1.3.Numerical.metho.ds.fo.r.a.rbitra.ry.shapTed.nanopa.rticles..........2.fo.w.rticles12.2.2.Plasmon30senso.rs..............A.rticle.s.3.1.plasmonic.rticle.......3.2.epa...............34..............1232.3paCoupledeasurementsnanopa.r.ticles........3.5.................39.Setup.e.................calib..........14.2.3.1.Dimers.ofSetupspheres................4.3.1.................47...................2.6.11rapping5rces2.3.2.Distance.sensing.with.dimers....................8.Optical.rces.et.een.nanopa............172.6.32.3.3.Conclusion..............................3.Single.pa.as.pH-Sen.o.33.Basic.of.nano.a.pH-sensing..........20.2.433NanopaSamplerticlerheatingration...............................3.3.measurements..........................20.2.55TSingleotalrinternalmreection.illumination........................7.Conclusion................24.2.5.1.Basic.concept............4.Fluctuation.41.Exp.rimental...............................41.Setup.ration..2.4.2.5.2.P.enetration.depth....................4.3.p.rmance..............................2456Noise2.6.Optical.fo.rces............................IX..4.3.2.Overall6.3.6microscop.e77colle.ctionreticaleci.ency..o.8.......dimers...................Senso48.4.3.3.Baseline.stabilization....w...Resp...e.............................ness.......7.348.4.4.Conclusion..Exp.....6.3.1.........avelength.....Reversibilit.......................bath.......dimers.......6.4....49.58Single.Unlab.eled.Molecule.Sensing751r5.1eDierent.confo.rmations.s.ensedp...................72.riments.strong.........p..............52755.1.1bleLa.y.er.sensing....75.of.............Monomer.............6.3.5.ientation.............8.emp......52.5.1.2.Au.sphere.attachment6.3.7.................retical...............6.5................53.5.2dimersSinglesunlabStele7.1dresultsp.rotein.detection..........7.2.of.stiness...........................X....53.5.2.1.Materials.and6.3methoedson.under.illumination...............73.Threshold.o.er......................54.5.2.2.Time.traces6.3.2shoonsiwingwsingleregimep.rotein.attachment................6.3.3.y.th.collapse..55.5.2.3.Control.exp.erimen.ts..........6.3.4.illumination.........................79.Dimer.r..........57.5.2.4.Simulation.of.exp.ected.shifts......0.T.erature.............................0.Silver60.5.2.5.Conclusions.and.future.p.otential..................80.Theo.investigation................64.5.3.Time.resolved.binding.kinetics.and1equilibConclusionrium.uc.tuations...................65.6.Nano.dimers.under.Strong83IlNanoluasmirnfoaSpacertii85onExp69rimental6.1.F.unctionalization.and.p.repa.ration..................86.Theo.calculation.the.olymer.................93.Conclusion69.6.2.App.ro.ximation.of.dimer.gap.distance..................95..