Genetic diversity of Helicobacter pylori isolates in Sudan [Elektronische Ressource] / vorgelegt von Wael Faroug Elamin

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• Gesundheit

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• Publié par: medizinische_hochschule_hannover_-mhh
• Publié le: 01 janvier 2008
• Nombre de lectures: 15
• Langue: Deutsch
• Poids de l'ouvrage: 44 Mo

Extrait

KhartoumAushscdemerInstitutvf?rErlangungMediziniscMedizinischevMikrobiologieFund2008KrankDoktorgradesenhaushanygieneHoderuleMediziniscvhenWHoElamincvhsczurhdesuledHannoMedizinvdererhenGeneticcDivhersitHannoyeroforgelegtHeliconobaelacterarougpyloriausIsolatesHannoinerSudanDissertationeAngenommenProf.vKreomt:Senat17.04.2009derDr.MediziniscranzhenJ?rgHomcProf.hscDr.hnulemed.HannoKvDr.ercamag17.4.2009henGedruchktHans-HeinricmiteGSuerbaumenehmigungStuhderbMediziniscDr.henFHoBangecoreferenhscProf.hmed.uleSHannohmidtkvTerderPr?siden?ndlict:Pr?fung:ProfessorPromotionsausscDr.ussmitglieder:med.Dr.DieterhBitter-SuermannipBetreuer:Prof.ProfessorSebastianDr.Prof.med.ManfredSebastianrmaSuerbaumn-SpangenReferenergt:terDeanddicyateydmtowifeMymPdaugharents,cialGeneticobservDivurersitmigrationsydofwhicHeliciobnactertopyloriofIsolatesWinectivSudanpyloriWofaeltFbarougcompariElaminWSubmittedsequenceforgenesthemutY,degreenewofallDoorctororiginMedicinaetheFtebruarysev2008theAbstractpHelicThisobaacterandpylorioinfectionisolofuhultiloumansypingishouseksoatpA,oldppthatyphCitsdenedpopulation,opulationpredominatesgeneticstrains,structureofreectsorigin.thatsuggestofancestralanciene)twhichinumananmigrations.migration.OriginsbacandogeneticsremainedofotherH.onlypyloriminimallyinpAfricaduearecalnotSudanwtheirellsdescribned.globalItates.isenotseclearmhocuswtdieren(MLST)t7weepinga(vefp,eseI,ofa,htrpC,uman).migrationseinaAfricaphahspEastAfrica,vheinconSudanesetributedirresptoethegeographicevethnicolutionOurofationsstrainthedivofersitH.y(hsp-EuropofinH.Sudan,pylorih.sTheagreemenobwithjectivouteAfricaofDespiteoureralstudykwtasSudan,tohspEastAfricalearndominanthewhilesancestraleopulationissuescobtributedy.studyingcouldtheossiblygeneticeoriginstoofmatriloH.sopyloripattern.inCopDeclarationtheTheofwknoorkaelinquotationsthisderivthesistraryis2008basedTheonwithresearcbhconsencarriedshouldouttheatthethetHelicyobElaminacteryrighresearcthesishauthor.group,itHannopublishedvpriorer,andGermanfromye.vNoconpartinoftext.thisyrighthesis?hasbbWeenF.submitted.elsewherecopfortanthisyrestsotherthedegreeNoorfromqualicationshouldandeitwithoutisauthor'sallwrittenmtyinformationoedwnitwborkacunlesswledged.referencedtoDr.Ak,cforknowwledgemenAtsHomeidaThisthanksstudyEltahirwtheothankuinlvidandnotconhaDr.vvidingerbSinaeenvidingmaden,pandossiblethewithouthisthewsupphortbacofChristianmandyiksupdaervisorursingProf.ofDr.rghanSebastianSudan.Suerbaum.terologyThroughfriendshiphisysupouldervisionProf.Illeague,wgas-asmeablethetoalsolearnwingvastabshironaluableScknoStefanwledgeobiasregardingeinitzborstenothSpmoleculartomiforcrobiologyuousasort.walsoelltoamsDr.hotheirwfortowiththinkbiopsiesprobleousramsforthrough.ortThealsolabtrooratoryinoftheProf.andDr.ySebastianforSuerbaumIhasebyeenarougahisvImaderyvidingpleas-sheepanallotwandlabaphasefunject.atmosphereProf.inrwhictohattocenstuSandradyhandarz,groKulicw;TmostScofwalerlTthough,SterzenIh.thankecialhimgoforDr.proKraftvidinghismetinwithadviceasupplifetimeIoppouldortunitlye.thankIHatialsoMu-thankwi,mM.A.yandco-supnervisor,staDr.proDanielmeFmostalush,theforandinitiatingYtheMiproijehectsuppandinforIhisthankconGas-tinenuousunitssuppIbnorthospital,andChineseguidance.hospitalIthewcademouldCharitlikhospitaleprotobiopsies.thankwPD.likDr.toChristinemJosenhansfather,andFmElamiyandcolcoleaguesProf.ofAradaibtheprogroupmeAnnatricLeybsampleso,forBirgitwingBrennektoe,orkClaudiatheirModuringccia,initialDanielaofG?ppproel,IJessikthankaM.Scfohalloulze,meNinauseLotzing,labPYatricmedicalkter.Olbermann,.Con.ten.ts.Abstract.iv.Declaration16v.A.c.kno(MtDNA)wledgemen.ts.vi.1.INTR.ODUCTION.1.1.1.Helic.obMiacter.pylori....i.......Ge...1.3.2.......20.....p.......hond.....1.2.2.......Mi.of..1.1.1.1.Hi.s.tory....................1.3.3.......proteins.........1.2.genetics...........13.o.al......1.1.1.2.GeChromosomeneral.c.haracteristics........5.a.routes...........Sudan.................17.y2.1.1.3.Di.sease.and.epidemiology....s.................opulation.............eeping.function..3.1.1.4.Ecol.ogical.nic.he....11.African.opulation.........................1.2.1.t.c.ri.DNA..........4.1.1.5.Ge.netic.div14ersitYy.of.Helic.ob.acter.pylori................1.1.2.3.gr.t.on.out5Africa1.1.6.Helic.ob.acter.pylori.in.Sudan......1.3................................8.1.1.7.Helic1.3.1obographacter.pylori.as.a.mark.er.of.h.uman.p.opulation.migra-.tions....17.Hi.tory...............................19.P.genetics....................9.1.1.8.Houseviik.Con32ten.t.sPCRviii.1.3.3.1rEthnic.g.r.oups........3.2.3.2.1.in.y.............strains.Ide...e..........20.1.3.3.251Language29groups......DNA-sequencing.......Data.........Hel.....from.patien...3.1.2.a..20.1.3.3.3.P.opulation.genetics........3.3.......5...Purication.duct...........29....21.1.4.Study.rationale....29.................3.Culture.p.Sudan.....32.tical..n.........p.from.patien......22.2ylogeneticMaterials.and.Metho.ds.24.2.1.Gastric.biopsiesAnalysis.............sp.............Discussion.58.....2.6.of.pro....................24.2.2.Sheep2.7gastric.D.NA.samples........................2.8.Analysis....................24.2.3.Culture......30.Results.3.1.of.icobacter.ylori.from.............3.1.1.n.isolates.di.e.e.t.ts.............37.Multi.l.strains.s25me2.4tPCR..............38.Ph.Analysis...........................44.Structure........................25462.5HelicobacterAgaroseeciesGelSheepElectrophoresis.for.Amplico.n.s..............4.53.Conclusion.Bibliograph.59.hListhpEuropofandFiguresof1.1seMigration.routesisooutisolatesofvAfricamap,(.F.orsterandandsequenced)Matsumurofaage,ancestry2005)v...comp....16using1.252LanguagehpSudan,familieshspMaori,andSudan.theirDistructdistributionrininAfricay(www.gmi.org)thin.estimated.ancestral21ts.3.1ositionDistribution.of.ivsolatestionwithin.Sudan......T.ts...n.hpAsi.hpAfrica2,.(subp.pEAsia)..are.isolates.45.graphic.prop.n.n.H.36determined3.2V2.0RAPDdel).PCRfor.indicates.t.eac.opulation.coloured.3.6.ancestral.Su.......3.7.wing.anc.s.groups.Africa...............50.pro.pairwise.......opulatio.s39e,3.3a2,HapplothpNEAfrica,ofhpAfrica1concatenatedhpEastAsiageneopulationsshohs-wing71platesolymorphicfromsites(69innewlypaired.isolates.3.5Vgure:erticalplotlinesthewithinothetiobsoancestralxesucleotidesindicate598thepyloripasosibtStructurei(linkonsmoofAse-linequenceeacpisolateolymorphisms.the.amoun.of.from.h.p.as.e.segmen.48.A.erage.comp.of.dane.strains...........49.Africa.sho.a.erage.estral40o3.4iNeighofbinour-joiningaroundtree.(Kim.ura.2-parameter).of.598.H..pylori.hap-.lot.yp.es.color-co.ded.according.to.p3.8opulationreeassignmenducedtBLASTbalignmeny.STR.UC-.TURE.in.to.theixp.List71of.TSUDSO4.2ables.2.1.Concenertrationscultureand3.3v.olumesofof3.2PCRoinmixthe.biopsies.........dierences.......and.58.the.the.ual.w.patien26.2.2.Primers./.anneal.ing.temp.eratures..P.....39.sequenced.......Cultured.represen.cultured.The.follo.um.cate.is.indivi.SUDSO4.1.that.dieren26the2.4wRAPDositivPCR.primers............tical...............38.isolates.genes.........Prop.fragmen.........4227.2.62916SIsolates:RNAisolatesPCRgeographicprimerstation..Isolates.from.individuals..p.ts.wing.n.b.indi-.that.isolate.from.same.d.eg.and.indicate.t.o.t.from.same.t.ere.p.e.28.2.5.Cycling.conditions....................35.Iden.Strains...............................3.428aired2.7andSequencinginreaction.v.olume.s............3.5.erties.gene.ts...................x.