Hyberbaric oxygen increases atresia in normal & steroid induced PCO rat ovaries

Atis Alev, Aydin Yavuz, Ciftci Filiz, Sakä±Z Damlanur, Arslan Abdullah, Toklu, Donmez Melahat, Goker, Goker Nimet

Description

In this study, we investigated the effect of hyperbaric oxygen therapy (HBOT) on the morphology of estradiol valerate (EV) induced polycystic ovary (PCO) to find a new treatment modality for improvement of PCO. Methods The rats were divided into four groups. Group1, control; group 2, PCO group; group 3, PCO with HBOT group and group 4, normal ovary with HBOT. PCO was induced by a single intramuscular injection of 4 mg EV in adult cycling rats. Other rats with normal ovaries had oil injection as placebo. HBOT was applied to third and fourth groups for six weeks. Histopathologic evaluation of ovaries of all groups were performed & compared. Results Six weeks of HBOT was resulted in increase in follicular atresia, decrease in the number of primary, secondary, tertiary follicles and decrease in the number of fresh corpus luteum in normal rat ovary. HBOT on polycystic rat ovary, resulted in significant increase in atretic follicles which were already present. Conclusions HBOT of six weeks itself, changed ovarian morphology in favor of atresia both in PCO group and control group. This result of aggravated follicular atresia after HBOT on EV induced PCO may be due to long-term exposure in our protocol which with this state seems to be inapplicable in the improvement of PCO morphology.

Sujets

• Rat

Informations

• Publié par: biomed
• Publié le: 01 janvier 2012
• Nombre de lectures: 10
• Langue: English
• Poids de l'ouvrage: 2 Mo

Extrait

Atiset al.Reproductive Biology and Endocrinology2012,10:11 http://www.rbej.com/content/10/1/11

R E S E A R C HOpen Access Hyberbaric oxygen increases atresia in normal & steroid induced PCO rat ovaries 1* 21 34 41 Alev Atis, Yavuz Aydin , Filiz Ciftci , Damlanur Sakız , Abdullah Arslan , Akın S Toklu , Melahat Donmezand 1 Nimet Goker

Abstract Background:In this study, we investigated the effect of hyperbaric oxygen therapy (HBOT) on the morphology of estradiol valerate (EV) induced polycystic ovary (PCO) to find a new treatment modality for improvement of PCO. Methods:The rats were divided into four groups. Group1, control; group 2, PCO group; group 3, PCO with HBOT group and group 4, normal ovary with HBOT. PCO was induced by a single intramuscular injection of 4 mg EV in adult cycling rats. Other rats with normal ovaries had oil injection as placebo. HBOT was applied to third and fourth groups for six weeks. Histopathologic evaluation of ovaries of all groups were performed & compared. Results:Six weeks of HBOT was resulted in increase in follicular atresia, decrease in the number of primary, secondary, tertiary follicles and decrease in the number of fresh corpus luteum in normal rat ovary. HBOT on polycystic rat ovary, resulted in significant increase in atretic follicles which were already present. Conclusions:HBOT of six weeks itself, changed ovarian morphology in favor of atresia both in PCO group and control group. This result of aggravated follicular atresia after HBOT on EV induced PCO may be due to longterm exposure in our protocol which with this state seems to be inapplicable in the improvement of PCO morphology. Keywords:Hyperbaricoxygen, Polycysticovary, Rat, Estradiol valerate

Background Polycystic ovary syndrome (PCOS), the most common endocrinopathy in women of reproductive age is a mul tifactorial metabolic disease associated with insulin resis tance [1]. PCOS is a common condition with a range of clinical features. These reproductive features include anovulation, irregular menstrual cycles, clinical and bio chemical hyperandrogenism and infertility. Metabolic features include increased risk factors for type 2 diabetes mellitus and cardiovascular disease and increase in the prevalence of the metabolic syndrome [1,2]. Previously, the diagnosis of PCOS was based on the National Institute of Health (NIH) criteria comprising biochemical or clinical hyperandrogenism and anovula tory irregular menstrual cycles with the exclusion of related reproductive disorders. In 2003, diagnostic guidelines for PCOS were expanded to the socalled

* Correspondence: [e-mail] 1 Sisli Etfal Training & Research Hospital Obstetrics & Gynecology, Istanbul, Turkey Full list of author information is available at the end of the article

Rotterdam criteria, now based on presentation with any two of the three criteria of hyperandrogenism, irregular anovulatory periods or polycystic ovaries on ultrasound [1,3]. The reported prevalence range of PCOS is between 2.2% to 26% [1,3,4]. The wide range of esti mated PCOS prevalence can be explained by different recruitment processes of the study populations, selection biases, ethnic and racial variations in addition to, the criteria used for its definition and the screening meth ods used to identify each criteria; considering the Rot terdam versus NIH criteria increase the PCOS prevalence by 2 times. The latest study by Tehrani et al. demonstrated that the prevalence’s of PCOS using the NIH definition were 8.5% [4]. The abnormalities detected in PCOS have been attrib uted to primary defects in the hypothalamicpituitary adrenal axis, the ovarian microenvironment, the adrenal gland and the insulin/insulinlike growth factor meta bolic regulatory system [2,5]. There is evidence that both hypothalamic and pituitary mechanisms contribute to the gonadotropin dysfunction in PCOS.

© 2012 Atis et al; BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.