Impaired fertility in transgenic mice overexpressing betacellulin [Elektronische Ressource] / by Ana Angélica Gratao

Ana Angelica Gratao

Description

Institute for Molecular Animal Breeding and Biotechnology, Gene Center Faculty of Veterinary Medicine of the Ludwig-Maximilians University, Munich Prof. Dr. Eckhard Wof Impaired Fertility in Transgenic Mice Overexpressing Betacellulin Thesis for the attainment of the title of Doctor in Veterinary Medicine from the Faculty of Veterinary Medicine of the Ludwig-Maximilians University, Munich by Ana Angélica Gratao from Ipameri, Brazil Munich, 2007 Aus dem Institut für Tierzucht der Tierärztlichen Fakultät der Universität München Lehrstuhl für Molekulare Tierzucht und Biotechnologie, Genzentrum Univ.-Prof. Dr. Eckhard Wof Fruchtbarkeitsstörungen bei transgenen Mäusen, die Betacellulin überexprimieren Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München von Ana Angélica Gratao aus Ipameri, Brasilien München, 2007 Gedruckt mit Genehmigung der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München Dekan: Univ.-Prof. Dr. E. P. Märtlbauer Referent: Prof. Dr. Wolf Korreferent(en): PD. Dr. Neubauer Tag der Promotion: 9. Februar 2007 Meinem Ehemann, Rudah Brentano Assef I CONTENTS Page CONTENTS I ABBREVIATIONS III 1.

Sujets

• Biologie

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2007
• Nombre de lectures: 11
• Langue: English

Extrait

Institute for Molecular Animal Breeding and Biotechnology, Gene Center Faculty of Veterinary Medicine of the Ludwig-Maximilians University, Munich Prof. Dr. Eckhard Wof         Impaired Fertility in Transgenic Mice Overexpressing Betacellulin   Thesis for the attainment of the title of Doctor in Veterinary Medicine from the Faculty of Veterinary Medicine of the Ludwig-Maximilians University, Munich               by Ana Angélica Gratao from Ipameri, Brazil  Munich, 2007 

Aus dem Institut für Tierzucht der Tierärztlichen Fakultät der Universität München Lehrstuhl für Molekulare Tierzucht und Biotechnologie, Genzentrum Univ.-Prof. Dr. Eckhard Wof         Fruchtbarkeitsstörungen bei transgenen Mäusen, die Betacellulin überexprimieren   Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München              von Ana Angélica Gratao aus Ipameri, Brasilien  München, 2007

Gedruckt mit Genehmigung der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München          

Dekan: Univ.-Prof. Dr. E. P. Märtlbauer Referent: Prof. Dr. Wolf Korreferent(en): PD. Dr. Neubauer   

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Tag der Promotion: 9. Februar 2007  

 

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Meinem

Ehemann,

Rudah

Bren

tano

Assef

 

I

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 CONTENTS  CONTENTS ABBREVIATIONS  1. INTRODUCTION AND OBJECTIVES 2. REVIEW OF THE LITERATURE 2.1 Growth Factors 2.1.1 The Epidermal Growth Factor Receptor Ligands 2.1.2 Betacellulin 2.2 Female Reproduction 2.2.1 Sexual Maturity 2.2.2 Oocyte maturation and Ovulation 2.2.3 Implantation 2.2.3.1 Role of EGFR family members in implantation 2.3 Male Reproduction 3. ANIMALS, MATERIALS AND METHODS 3.1. Animal Breeding 3.2 Mouse genotyping 3.3 Analysis of Body and Organs Weight 3.4 Fertility Analysis 3.5 Evaluation of female puberty onset and estrous cycle tracking 3.6 Embryo Implantation studies 3.7 Ovulation and Fertilization analysis 3.8 Cumulus oocyte complex isolation and culture  3.9 Sperm isolation andin vitrofertilization 3.10 Sperm analysis 3.11 Histological and Immunohistochemical studies 3.12 Statistical analysis 4. RESULTS 4.1 Analysis of Body and Organs Weight 4.2 Fertility measurements

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Page I  III 

1 3  3 4 5 10 12 14 15 16 19 22  22 22 25 26 26 27 27 27 28 28 29 29 30  30 31

 

II

4.2.1 Males 4.2.2 Females 4.3 Evaluation of female puberty onset and estrous cycle tracking 4.4 Embryo Implantation 4.5 Ovulation and Fertilization analysis 4.6In vitromaturation 4.7In vitro fertilization  4.8 Sperm analysis  4.9 Immunohistochemical analysis 5. DISCUSSION 5.1 Males  5.2 Females 5.2.1 Fertility Analyses 5.2.2 Female Puberty Onset and Estrous Cycle Tracking 5.2.3 Embryo Implantation  5.2.4 Ovulation and Fertilization Ratesin vivo 5.2.5In vitroMaturation and Fertilization 6. SUMMARY 7. ZUSAMMENFASSUNG 8. BIBLIOGRAPHY

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31 31 32 34 35 36 37 38 39 41  41 43 43 43 44 45 46 48  50  53 

 

ABBREVIATIONS  ADAM AREG  BMP-15 BSA BTC  BTC Btc Btc CCOs DAB DNA DNerbB4 dNTPs E  E 2 EDTA EGF  EGFR EPGN ErbB1-4 EREG  Erk ERα  FCS FGF  FSH GnRH HBEGF  hCG HGF hTGFA  icv

III

Adisintegrinandmetalloprotease Amphiregulin  Bone morphogenetic protein 15 Bovine serum albumin Betacellulin, protein, human  Betacellulin, gene, human  Betacellulin, protein, mouse  Betacellulin, gene, mouse  Complexes Cumulus-Oocytes diaminobenzidine Deoxyribonucleic acid Dominant-negative erbB4 receptor deoxyribonucleoside triphosphates Estrogen  Estradiol Ethylene diamine tetraacetic  Epidermal Growth Factor  Epidermal Growth Factor Receptor Epigen Receptor tyrosine kinases 1-4 Epiregulin  Extracellular signal-regulated protein kinase Estrogen receptor  Fetal calf serum Fibroblast Growth Factor  Follicle-stimulating hormone Gonadotropin releasing hormone Heparin-binding EGF-like  Human chorionic gonadotropin Hepatocyte Growth Factor Human transforming growth factor-α  Intracerebroventricular