An elevation in intramucosal–arterial P CO 2 gradient (ΔP CO 2 ) could be determined either by tissue hypoxia or by reduced blood flow. Our hypothesis was that in hypoxic hypoxia with preserved blood flow, ΔP CO 2 should not be altered. Methods In 17 anesthetized and mechanically ventilated sheep, oxygen delivery was reduced by decreasing flow (ischemic hypoxia, IH) or arterial oxygen saturation (hypoxic hypoxia, HH), or no intervention was made (sham). In the IH group ( n = 6), blood flow was lowered by stepwise hemorrhage; in the HH group ( n = 6), hydrochloric acid was instilled intratracheally. We measured cardiac output, superior mesenteric blood flow, gases, hemoglobin, and oxygen saturations in arterial blood, mixed venous blood, and mesenteric venous blood, and ileal intramucosal P CO 2 by tonometry. Systemic and intestinal oxygen transport and consumption were calculated, as was ΔP CO 2 . After basal measurements, measurements were repeated at 30, 60, and 90 minutes. Results Both progressive bleeding and hydrochloric acid aspiration provoked critical reductions in systemic and intestinal oxygen delivery and consumption. No changes occurred in the sham group. ΔP CO 2 increased in the IH group (12 ± 10 [mean ± SD] versus 40 ± 13 mmHg; P < 0.001), but remained unchanged in HH and in the sham group (13 ± 6 versus 10 ± 13 mmHg and 8 ± 5 versus 9 ± 6 mmHg; not significant). Discussion In this experimental model of hypoxic hypoxia with preserved blood flow, ΔP CO 2 was not modified during dependence of oxygen uptake on oxygen transport. These results suggest that ΔP CO 2 might be determined primarily by blood flow.
Open Access Research Intramucosal–arterial PCOgap fails to reflect 2 intestinal dysoxia in hypoxic hypoxia 1 2 3 4 5 2 Arnaldo Dubin , Gastón Murias , Elisa Estenssoro , Héctor Canales , Julio Badie , Mario Pozo , 4 6 7 8 Juan P Sottile , Marcelo Barán , Fernando Pálizas and Mercedes Laporte
1 Principal Investigator, Cátedra de Farmacologia, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina 2 Research Fellow, Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina 3 Medical Director, Servicio de Terapia Intensiva, Hospital San Martín de La Plata, Argentina 4 Staff physician, Servicio de Terapia Intensiva, Hospital San Martín de La Plata, Argentina 5 Research Fellow, Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina 6 Medical Director, Unidad de Transplante Renal, CRAI Sur, CUCAIBA, Argentina 7 Medical Director, Servicio de Terapia Intensiva, Clínica Bazterrica de Buenos Aires, Argentina 8 Director, Servicio de Laboratorio, Hospital San Martín de La Plata, Argentina
Abstract IntroductionAn elevation in intramucosal–arterial PCOgradient (ΔPCO) could be determined either 2 2 by tissue hypoxia or by reduced blood flow. Our hypothesis was that in hypoxic hypoxia with preserved blood flow,ΔPCOshould not be altered. 2 MethodsIn 17 anesthetized and mechanically ventilated sheep, oxygen delivery was reduced by decreasing flow (ischemic hypoxia, IH) or arterial oxygen saturation (hypoxic hypoxia, HH), or no intervention was made (sham). In the IH group (nblood flow was lowered by stepwise= 6), hemorrhage; in the HH group (nhydrochloric acid was instilled intratracheally. We measured= 6), cardiac output, superior mesenteric blood flow, gases, hemoglobin, and oxygen saturations in arterial blood, mixed venous blood, and mesenteric venous blood, and ileal intramucosal PCOby tonometry. 2 Systemic and intestinal oxygen transport and consumption were calculated, as wasΔPCO. After basal 2 measurements, measurements were repeated at 30, 60, and 90 minutes. ResultsBoth progressive bleeding and hydrochloric acid aspiration provoked critical reductions in systemic and intestinal oxygen delivery and consumption. No changes occurred in the sham group. ΔPCO± 10 increased in the IH group (12 versus 40 [mean ± SD] ± 13 mmHg;P<0.001), but 2 remained unchanged in HH and in the sham group (13 ± 6 versus 10 ± 13 mmHg and 8 ± 5 versus 9 ± 6 mmHg; not significant). DiscussionIn this experimental model of hypoxic hypoxia with preserved blood flow,ΔPCOwas not 2 modified during dependence of oxygen uptake on oxygen transport. These results suggest thatΔPCO 2 might be determined primarily by blood flow.
Introduction Tonometry is one of the few clinical tools available for the monitoring of tissue oxygenation [1]. Decreases in gastro
intestinal intramucosal pH (pH ) have usually been considered i as indicators of dysoxia [2–5]; that is, as heralds of insufficient O to meet tissue demands. Recently, the intramucosal– 2
ANOVA = analysis of variance; CO = cardiac output; DO= oxygen transport;ΔPCO= intramucosal–arterial PCOgradient; FO= fraction of 2 2 2 i 2 inspired oxygen; HH = hypoxic hypoxia; IH = ischemic hypoxia; pH = intramucosal pH; VO= oxygen uptake. i 2