Mapping of new microsatellite markers and molecular identification of quantitative trait locus (QTL) for agronomically important traits in barley [Elektronische Ressource] / vorgelegt von Jingzhao Li

Sujets

• Biologie

Informations

• Publié par: martin-luther-universitat_halle-wittenberg
• Publié le: 01 janvier 2004
• Nombre de lectures: 27
• Langue: Deutsch

Extrait

Aus dem Institut für Pflanzenzüchtung und Pflanzenschutz


“Mapping of new microsatellite markers
and molecular identification of quantitative trait locus (QTL)
for agronomically important traits in barley”




Der Landwirtschaftlichen Fakultät
der
Martin-Luther-Universität
Halle-Wittenberg
als
Dissertation
zur Erlangung des akademischen Grades
doctor agriculturarum (Dr. agr.)
vorgelet von
M. Sc. In bio.
Jingzhao Li
geb. am 28. November, 1972
in Beijing, Volksrepublik China


Gutachter: Prof. Dr. W. E. Weber
Dr. habil. M. Ganal
PD Dr. A. Börner

Eröffnung des Promotionsverfahrens: 22 Juni 2004
Datum der öffentlichen Verteidigung: 6 Dezember 2004

Halle/Saale
urn:nbn:de:gbv:3-000007640
[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000007640]



Content


1 Summary ...........................................................................................................................1
2 Zusammenfassung ............................................................................................................3
3 Literature review ..............................................................................................................5
3.1 Yesterday and today of barley....................................................................................5
3.2 Molecular mapping of the barley genome..................................................................7
3.2.1 Genetic mapping in barley..................................................................................7
3.2.2 Molecular markers..............................................................................................8
3.2.2.1 RFLP-markers................................................................................................
3.2.2.2 RAPD-m...............................................................................................9
3.2.2.3 AFLP-markers9
3.2.2.4 STS-markers.................................................................................................10
3.2.2.5 SSR-markers11
3.2.2.6 SNP-markers12
3.3 Mapping of quantitative trait loci (QTL)..................................................................14
3.3.1 Mapping and analysis of quantitative trait loci (QTL).....................................14
3.3.1.1 Single-marker tests.......................................................................................14
3.3.1.2 Single interval mapping (SIM).....................................................................15
3.3.1.3 Composite interval mapping (CIM) .............................................................16
3.3.2 QTLs in barley..................................................................................................16
3.3.2.1 Mapping of agronomic traits ........................................................................16
3.3.2.2 Mapping of malting quality ..........................................................................17
3.3.2.3 Mapping disease resistance gene..................................................................19
3.4 Contribution of wild barley to crop improvement....................................................21
3.5 Advanced backcross-QTL analysis23
4 Mapping of new microsatellite markers.......................................................................25
4.1 Introduction..............................................................................................................25
4.2 Materials and methods..............................................................................................26
4.2.1 Molecular markers............................................................................................
4.2.2 Plant materials..................................................................................................26
4.2.3 Plant DNA extraction .......................................................................................27
4.2.4 PCR reaction.....................................................................................................27
4.2.5 Analysis of PCR products ................................................................................
4.2.6 Analysis and mapping of microsatellites..........................................................28
4.3 Results......................................................................................................................29
4.3.1 Screening for polymorphism and diversity analysis.........................................29
4.3.2 Mapping of microsatellite loci..........................................................................32
4.4 Discussion.................................................................................................................37
i ii Content
5 QTL mapping ................................................................................................................. 43
5.1 Introduction..............................................................................................................43
5.2 Mapping QTL in a BC -DH population from a cross ‘Brenda’ × HS213 ............... 44 3
5.2.1 Materials and methods.....................................................................................44
5.2.1.1 Population development...............................................................................
5.2.1.2 Fine mapping of QTL for heading date ....................................................... 44
5.2.1.3 Field tests and evaluation of agronomic traits, diseases resistance and
malting quality ............................................................................................................. 44
5.2.1.4 Genotyping and linkage analysis ................................................................. 46
5.2.1.5 QTL analysis and statistical analysis ........................................................... 46
5.2.2 Results47
5.2.2.1 Microsatellite polymorphism and marker segregation.................................
5.2.2.2 Distributions of traits ................................................................................... 48
5.2.2.3 Correlations between traits and ANOVA for genotype and environments . 49
5.2.2.4 QTL detection..............................................................................................50
5.2.2.5 Mapping of hd2.2 on the short arm of chromosome 2H.............................. 54
5.2.3 Discussion........................................................................................................57
5.2.3.1 Clustering of QTLs detected in this study ...................................................
5.2.3.2 Comparisons with other QTL studies in barley ........................................... 58
5.3 Mapping of QTL in a BC population from a cross ‘Brenda’ × HS584 .................. 63 3
5.3.1 Materials and methods.....................................................................................63
5.3.1.1 Population development...............................................................................
5.3.1.2 Field trials and evaluation of agronomic traits............................................. 63
5.3.1.3 Genotyping and linkage analysis ................................................................. 64
5.3.2 Results..............................................................................................................64
5.3.2.1 Microsatellite polymorphism and marker segregation.................................
5.3.2.2 Distributions of traits ................................................................................... 65
5.3.2.3 Correlations between traits and ANOVA analysis ...................................... 65
5.3.2.4 QTL detection..............................................................................................67
5.3.3 Discussion........................................................................................................71
5.3.3.1 Clustering of QTLs detected in this study ...................................................
5.3.3.2 Comparisons of the AB-QTL analyses between ‘Brenda’ × HS213 and
‘Brenda’ × HS584 with other QTL analyses in barley ................................................ 73
6 Conclusions and outlooks 78
7 References....................................................................................................................... 80
Acknowledgements ................................................................................................................99
Curriculum vitae... 100
Publications .......................................................................................................................... 101
ERKLÄRUNG...................................................................................................................... 102


Abbreviations
A adenine
AB advanced backcross
AFLP amplificated fragment length polymorphism
BC first backcross generation 1
bp basepair
C cytosine
cDNA complementary DNA
CIM compound interval mapping
cM centiMorgan
DAF DNA amplification fingerprinting
DH doubled haploid
DNA deoxyribonucleic acid
EDTA ethylenediaminetetraacetic acid
EST expressed sequence ta