712 pages
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Final scientific reports of all contracts completed before August 1996

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Medical and biological research

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Publié par
Nombre de lectures 164
Langue English
Poids de l'ouvrage 18 Mo

EUROPEAN
COMMISSION
SCIENCE
RESEARCH
DEVELOPMENT
Biotechnology
«SfA (1992-1994)
Edited by
A. Hoeveler, M. Creşti f ¿TÌ
Final
Report
Vol. II
EUR 16922 EN Cilindroeca closterium (diatom): positive binding by a "sandwich procedure" with a species-
specific polyclonal primary mouse antibody and a secondary FITC labelled goat antimouse
antibody. The photo was kindly provided by the laboratory of Prof. Costas. Univ. Complutense de
Madrid, participant of the concerted action "Exploration of microbial diversity" B102 CT 930119,
coordinated by M.G. Hölle, Gesellschaft für Biotechnisch Forschung. European Commission
BIOTECHNOLOGY PROGRAMME
(1992-1994)
Final Report Vol. II
Edited by
A. Hoeveler, M. Creşti
PARI EURO?. BiYrth,
N.C.
C I EUR 16922 EN Published by the
EUROPEAN COMMISSION
Directorate-General XII
Science, Research and Development
LEGAL NOTICE
Neither the European Commission nor any person acting on behalf of the
Commission is responsible for the use which might be made
of the following information
Cataloguing data can be found at the end of this publication
Luxembourg: Office for Official Publications of the European Communities, 1997
ISBN 92-828-0058-X
© European Communities, 1997
Reproduction is authorized, except for commercial purposes, provided the source
is acknowledged.
Printed in Italy FINAL REPORT VOL II III
Biotechnology Programme (1992­1994)
FINAL REPORT VOL Π
IntroductionXI
Composition of the Committee XVIII
Area 1. Molecularapproaches
1.1Proteinstructureand function1
1.2GeneStructure113
1.3Expressionofgenes133
Area 2. Cellularandorganismapproaches
2.1 Cellular regeneration, reproduction and development of living
organisms 187
2.2Metabolismofanimals, plants and microbes: essential physiologi­
caltraits237
2.3Communicationsystems within living matter 437
Area 3. Ecology and population biology
3.1 Ecological implications of biotechnology491
3.2 Conservation of genetic resources 539
Area 4. Horizontal activities commontoallareas
4.2 Information infrastructure581
Coordinates of participants 603
Keyword index671
Participant index679
Contract numberindex000 TABLE OF CONTENTS V
TABLE OF CONTENTS
Introduction XI
Composition of the Committee XVII
Area 1 - Molecular Approaches 1
1.1 Protein structure and function 1-111
CT 930076 Structure/function studies of the cytochrome bf complex by biochemical,
biophysical and molecular genetic techniques 5
CT 930078 Structure and function of photosynthetic membrane H+ATPases 1
CT 930145 Drug, antibiotic and toxin excretion mechanisms in pro-and eukaryotic cells
cells 17
CT 930348 Membrane bound ATPases involved in the translocation of hydrophobic
molecules 2
CT 930364 Structure and function of NADH: ubiquinone oxidoreductase (complex 1). 25
CT 930422 Combines structural mechanistic and functional analysis of two similar
eucaryotic transport ATPases: the H+ and CA2+ pumps 29
1.1.3 Structure-function relationships of receptors 33
CT 930005 Structural and functional analysis of the interactions of tyrosine kinase
receptors with the conserved guanine nucleotide releasing
CT 930083 Molecular mechanisms of Beta-adrenergic receptor function and regulation
(Euroceptor) 37
CT 930102 Interactions of activins with type II activin receptors in differentiation
systems: structure-function analysis and synthesis of dominant negative
ligands and receptors 41
CT 930243 Structure and function of glutamate receptors
CT 930518 Functional architecture of the acetylcholine nicotinic receptor as a model of
ligand gated ion channel: investigations at the amino acid level 53
1.1.4 Metalloproteins 5
CT 942052 Novel Heam-based catalysis
CT 942060 Cytochrome P450-ligand interactions 61
1.1.5 Carbohydrate active enzymes5
CT 943008 New analyses through gene manipulation designed by crystallography,
structure comparison, and affinity labelling (NEWAMASE) 6
CT 943018 Structure-function relationships in the enzymatic degradation of cellulose
by fungal and bacterial cellulases9
CT 943025 Carbohydrate recognition and control by glycogen Phosphorylase and other
enzymes of carbohydrate metabolism and the design of potential antidi­
abetic drugs 73
CT 943030 The mechanism of crystalline cellulose hydrolysis by cellobiohydrolase I
and II of Trichoderma reesei
CT 943071 Structure-function relationships of glucosyltransferase 8VI TABLE OF CONTENTS
1.1.6 Lipases 87
CT 943013 Towards understanding of lipase (phospho) lipid interactions at the atomic
and molecular level
CT 943016 Structure function lipase relationships in low water media 91
CT 943041 Interactions, at the molecular level, of biologically important lipids and
inhibitors with lipases of medical interest: 9
- exocrine digestive lipases: the Pancreatic Lipase/Colipase complex,
Gastric Lipase and Bile-salt simulated Lipase
- Endocrine lipases: Lipoprotein Lipase and Hormone Sensitive Lipase... 9
CT 943063 A multidisciplinary approach to the structural analysis of the interaction of
phosphoinositide — specific phospholipa.se C with lipid substrate and
regulatory G-proteins 109
1.2 Gene structure 113-132
1.2.1 Saccaromyces cerevisiae 117
CT 930022 Experimental pilot study for a European cooperation on gene function
search in the yeast Saccharomyces cerevisiae 11
1.2.2 Arabidopsis thaliana 121
CT 930075 European scientists sequencing Arabidopsis "ESSA" 12
1.2.3 Bacillus subtilis9
CT 930272 Sequencing of the Bacillus subtilis genome
CT 942011g of the Bacillus subtilis genome 13
1.3 Expression of genes 133-186
1.3.1n of genes 137
CT 930060 Analysis of the transcriptional regulation of vertebrate Hox — 2.8/1.11
subgroup genes
CT 930101 Transcriptional control of phenylpropanoid metabolism 141
CT3 Liver transcription factors HNFl and HNF4 structure, function and
interrelationship 145
CT 930315 The regulation of the GATA proteins and their role in haematopoietic
differentiation9
CT 930319 The HNF3/ forkhead and HNFl transcription factor families: structural/
functional analysis and developmental role 153
CT 930345 In vitro selection of RNA structural motifs with self-splicing activity 157
CT 930450 Role of the PML, PML/RAR~ and RAR~ and nuclear proteins during the
myeloid differentiation process 161
CT 930454 Mechanisms of transcriptional activation and developmental role of tran­
scription factor TTF-l7
CT 930457 Characterization of the ^-amatinin resistant RNA polymerase associated
with high level transcription in Baculovirus-infeeled insect cells 17
CT 930473 Control of gene expression by nuclear receptors: interaction with ligands,
DNA and other factors 175
CT 943069 Animal Cell Biotechnology9 TABLE OF CONTENTS VII
Area 2 - Cellular and Organism Approaches 187
2.1 Cellular regeneration, reproduction and development of living
organisms 187-235
2.1.1 Control of development, cell commitment and cell totipotency in farm
animals of major economic importance 191
CT 930430 Identification of genes involved in early fish development 19
CT 943044 The pig gene mapping project (PIGMAP) — Identifying trait genes 193
2.1.4 In vitro developmental toxicology 209
CT 930107 A new strategy for developmetnal toxicology in vitro using molecular and
cellular markers in mammalian whole embryo culture to enhance sensitivity
and selectivity
CT 930108 Neurotoxicological systems 213
CT 930224 Development of in vitro neural and related immune systems for the study
of potentially toxic effects of novel and highly specific compounds during
cell differentiation7
CT 930394 Development of a new test of developmental toxicology 225
CT 930471 The use of gene transfer technology in conjunction with primary and clonal
culture for the establishment of predictive in vitro screening assays for
teratogenic potential: development, validation and transfer to the industrial
sector 231
2.2 Metabolism of animals, plants and microbes: essential physiological
traits 237-435
2.2.1 Transgenic animals and human health 24
CT 942002 Transgenic animal models for human ost osteoarthritis and
chonodrodysplasias
CT 942092 Modelling arthritis in transgenic mice3
2.2.2 Plants 249
CT 930400 Plant molecular genetics for an environmentally compatible agriculture.... 24
2.2.5 Mechanisms governing essential biological functions/metabolic activities in
filamentous fungi 375
CT 930008 Study of the mechanisms of secretion of heterologous proteins in
filamentous fungi as a knowledge base for efficient protein production 37
CT 930174 pH regulation, carbon catabolite repression, secreted en/ymes and
secondary metabolites in filamentous fungi 377
CT 930502 Protein secretion in filamentous fungi Trichoderma 383 VIII TABLE OF CONTENTS
2.2.6Mechanisms governing essential biological functions/metabolic activities in
industrial microbial species 385
CT930254Characterizationand optimisation of the Bacillus sitbtilis protein secretion
apparatus385
CT 930470Earlystepsinprotein secretion391
CT 943055Improvementoflactic acid bacteria for traditional and novel applications in
biotechnology395
2.2.7 Mechanismsofgeneregulation for the expression of economically impor­
tant products 417
CT942045Factors influencing efficient expression and secretion of heterologous
proteins in filamentous fungi417
CT942067Mechanisms of gene regulation for the polyketide and related metbolitc
production421
CT942100Regulation of antibiotic formation in filamentous fungi 425
2.2.8 Biochemical, physiological and genetic aspects ofrepresentative types of
extremophile organisms 429
CT 930274Biotechnologyofextremophiles429
2.3 Communicationsystemswithin living matter 437­490
2.3.1 New immunologicalapproaches todevelopeffective, safe and preferably
economicvaccines441
CT 920131Newstrategiesforthe development oforalvaccines: vectors based on non­
pathogenic commensal bacteria, synthetic peptides and lipophilic adjuvants. 441
CT 920321 Strategies for the design of peptide vaccines for the control of major human
infections 445
CT 930105 An investigation into the mechanism of adjuventicity of
immuno­stimulating complexes (ISCOMS) of defined chemical
compositionusing a primate model of Epstein­Barr virus induced
lymphoma449
CT 930111Technologiesforoptimisation of mucosal immune responses 453
CT 930349Newvaccinesbased on an optimal reconstruction ofimmunogenic
structurestargetedfor systemic or oral delivery 457
CT 930489Antigenpresentation for induction of optimal Τ cell­mediatedimmune
responses461
2.3.2 Development of in vitro human immuneresponses,eitherhumoral or
cellular, for fundamental and/or applied research465
CT 920363 In vitro study of the human immune response to the pollen allergens of
Parietaria 465
2.3.4 Coordinationofresearchon molecular, biochemical and pharmacological
approachesforthestudyof nerve and glial cells, their interactions and
dysfunctions467