Model membrane interactions with ions and peptides at the air/water interface [Elektronische Ressource] / von Elena Maltseva

Maltseva

Informations

• Publié par: universitat_potsdam
• Publié le: 01 janvier 2005
• Nombre de lectures: 6
• Langue: English
• Poids de l'ouvrage: 5 Mo

Extrait

Model membrane interactions with ions and peptides at the air/water interface








Dissertation
zur Erlangung des akademischen Grades
"doctor rerum naturalium"
(Dr. rer. nat.)
in der Wissenschaftsdisziplin "Physikalische Chemie"










eingereicht an der
Mathematisch-Naturwissenschaftlichen Fakultät
der Universität Potsdam




von
Elena Maltseva





Potsdam, den 28 Februar 2005 Contents
1. INTRODUCTION ....................................................................................................................................................4
2. THEORETICAL BACKGROUND. .......................................................................................................................6
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3. MATERIALS AND METHODS...........................................................................................................................16
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3.2.1. CD-spectroscopy.......................................................................................................................................18
3.2.2. Fluorescence microscopy..........................................................................................................................20
3.2.3. GIXD.........................................................................................................................................................21
3.2.4. X-ray reflectivity .......................................................................................................................................23
3.2.5 IRRAS.........................................................................................................................................................24
4. STRUCTURE AND IONIC INTERACTIONS OF DPPG MONOLAYERS. ..................................................31
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4.2.1 Surface pressure/area isotherms................................................................................................................32
4.2.2 X-ray Diffraction........................................................................................................................................34
4.2.3 X-ray Reflectivity .......................................................................................................................................37
4.2.4 IRRAS.........................................................................................................................................................39
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5. INTERACTIONS OF LIPID MONOLAYERS WITH FUSOGENIC PEPTIDE B18 ....................................45
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5.2.1 B18 structure in aqueous solutions............................................................................................................46
5.2.2 Adsorption of B18 at the air/water interface and to the lipid monolayers.................................................49
5.2.3 Structure of lipid monolayers with adsorbed B18 (GIXD).........................................................................52
5.2.4 IRRAS of B18 adsorbed at the air/water interface and on lipid monolayers. ............................................54
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6. INTERACTIONS OF Aβ (1-40) PEPTIDE WITH LIPID MONOLAYERS ...................................................66
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6.2.1 Aβ (1-40) structure in aqueous solutions...................................................................................................69
6.2.2. Adsorption of Aβ (1-40) peptide at the air/water interface and on lipid monolayers...............................71
6.2.3. Structure of lipid monolayers with adsorbed Aβ (1-40) peptide (GIXD)..................................................77
6.2.4. IRRAS of Aβ adsorbed at the air/water interface and on lipid monolayers..............................................83
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7. CONCLUSIONS.....................................................................................................................................................97
APPENDIX 1 ............................................................................................................................................................100
APPENDIX 2 ............................................................................................................................................................101
APPENDIX 3 ............................................................................................................................................................103
REFERENCES. ........................................................................................................................................................114
ACKNOWLEDGEMENTS .....................................................................................................................................122

, 1. Introduction
344546