Molecular basis of inter- and intraspecific multicellularity in prokaryotes [Elektronische Ressource] / vorgelegt von Roland Wenter

Roland Wenter

Sujets

• Biologie

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2010
• Nombre de lectures: 10
• Langue: English
• Poids de l'ouvrage: 7 Mo

Extrait

Molecular basis of inter- and intraspecific
multicellularity in prokaryotes



Dissertation der Fakultät für Biologie
der Ludwig-Maximilians-Universität München







vorgelegt von
Roland Wenter
am 04. Februar 2010


































1. Gutachter: Prof. Dr. Jörg Overmann
2. Gutachter: Prof. Dr. Dirk Schüler
Tag der mündlichen Prüfung: 12. Mai 2010

„In der Natur ist die Bedeutung des unendlich Kleinen unendlich groß“
Louis Pasteur, französischer Mikrobiologe (1822-1895)





























Meinen Eltern gewidmet
Publications originating from this thesis

Chapter 3:

Vogl, K., Wenter, R., Dressen, M., Schlickenrieder, M., Plöscher, M., Eichacker, L.A., Overmann,
J. (2008) Identification and analysis of four candidate symbiosis genes from
"Chlorochromatium aggregatum", a highly developed bacterial symbiosis. Environ Microbiol
10: 2842-2856

Chapter 4:

Wenter, R., Hütz, K.A., Dibbern, D., Plöscher, M., Li, T., Reisinger, V., Plöscher, M., Eichacker,
L.A., Eddie, B., Hanson, T., Bryant, D.A., Overmann, J. (2009) Expression-based
identification of genetic determinants of the bacterial symbiosis "Chlorochromatium
aggregatum". Environ Microbiol (published online ahead of print on April 01, 2010;
doi:10.1111/j.1462-2920.2010.02206.x)

Chapter 5:

Wenter, R., Wanner, G., Schüler, D., Overmann, J. (2009) Ultrastructure, tactic behavior and
potential for sulfate reduction of a novel multicellular magnetotactic prokaryote from North
Sea sediments. Environ Microbiol 11: 1493-1505

Contributions of Roland Wenter to the publications listed in this thesis

Chapter 3:
Roland Wenter performed the inital Northern blot analyses of ORFs Cag_1919 and 1920 as well as
the long range RT-PCR of Cag_1919. He also prepared the protein bands for mass spectrometry and
conducted the bioinformatics data analysis. The cultures of Chlorobium chlorochromatii CaD and
"Chlorochromatium aggregatum" were maintained together with Kajetan Vogl. Roland Wenter
wrote the respective experimental procedure and results sections and created Figure 1B.

Chapter 4:
Roland Wenter performed the in silico subtractive hybridisation analysis, prokaryotic cDNA
suppression subtractive hybridisation, reverse transcription, quantitative real-time PCR and
phylogenetic analysis of ORF Cag_1285. He conducted the cross-linking studies of consortia
membrane proteins as well as the isolation of chlorosomes and analysis of chlorosomal proteins
together with Katharina Hütz. Two-dimensional difference gel electrophoresis of the cytosolic
proteome was performed together with Veronika Reisinger and Dörte Dibbern. Roland Wenter
prepared the proteins for mass spectrometry as well as the cDNA for Illumina whole transcriptome
sequencing and subsequently conducted the bioinformatics data analyses. Chlorobium
chlorochromatii CaD and "Chlorochromatium aggregatum" were cultivated together with
Katharina Hütz and Dörte Dibbern. Roland Wenter and Jörg Overmann created all figures and
tables and wrote the publication.

Chapter 5:
Field sampling of the magnetic multicellular prokaryotes (MMPs) was done together with Prof. Dr.
Dirk Schüler. Roland Wenter conducted the magnetotactic enrichment of the MMPs, phase contrast
microscopy and the chemotaxis assays. Furthermore he performed fluorescence in situ hybridisation
and phylogenetic analyses of the MMPs 16S rRNA genes as well as the DsrAB and AprA gene
products. Scanning electron microscopy was carried out together with Prof. Dr. Gerhard Wanner.
Roland Wenter and Jörg Overmann created all figures and wrote the publication.


I herby confirm the above statements

________________________ _______________________
Roland Wenter Prof. Dr. Jörg Overmann
Contents

Chapter 1: Summary..........................................................................................................................1

Chapter 2: Introduction.....................................................................................................................5
Inter- and intraspecific interactions and symbioses between prokaryotes.....................................5
The phototrophic consortium "Chlorochromatium aggregatum" and
magnetotactic multicellular prokaryotes as model systems for bacterial multicellularity...........11
Aims of the present study............................................................................................................18
References....................................................................................................................................19

Chapter 3: Identification and analysis of four candidate symbiosis genes
from "Chlorochromatium aggregatum", a highly developed bacterial symbiosis................25
Summary......................................................................................................................................25
Introduction..................................................................................................................................26
Results..........................................................................................................................................27
Discussion....................................................................................................................................38
Experimental procedures.............................................................................................................42
References....................................................................................................................................48
Supplementary material...............................................................................................................54

Chapter 4: Expression-based identification of genetic determinants of the bacterial
symbiosis "Chlorochromatium aggregatum"............................................................................57
Summary......................................................................................................................................57
Introduction..................................................................................................................................58
Results..........................................................................................................................................59
Discussion....................................................................................................................................71
Experimental procedures.............................................................................................................78
References....................................................................................................................................86
Supplementary material...............................................................................................................92

Chapter 5: Ultrastructure, tactic behavior and potential for sulfate reduction of a
novel multicellular magnetotactic prokaryote from North Sea sediments.........................115
Summary....................................................................................................................................115
Introduction................................................................................................................................116
Results and discussion...............................................................................................................117
Experimental procedures...........................................................................................................129
References..................................................................................................................................135
Supplementary material.............................................................................................................140
Chapter 6: Discussion.....................................................................................................................141
Epibiont symbiosis genes related to bacterial virulence factors and exopolysaccaride
synthesis have functional implications for prokaryotic multicellularity....................................141
The fraction of unique epibiont genes is small due to the preadaptation of free-living
relatives to interspecific interaction..........