Max-Planck Institut für Kolloid- und Grenzflächenforschung Theorie und Bio-Systeme Phase Separation in Giant Vesicles Dissertation zur Erlangung des akademischen Grades "doctor rerum naturalium" (Dr. rer. nat.) in der Wissenschaftsdisziplin “Weiche Materie“ eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam von Yanhong Li Potsdam, im November 2008 Published online at the Institutional Repository of the University of Potsdam: http://opus.kobv.de/ubp/volltexte/2009/2913/ urn:nbn:de:kobv:517-opus-29138 [http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-29138] Abstract Giant vesicles may contain several spatial compartments formed by phase separation within their enclosed aqueous solution. This phenomenon might be related to molecular crowding, fractionation and protein sorting in cells. To elucidate this process we used two chemically dissimilar polymers, polyethylene glycol (PEG) and dextran, encapsulated in giant vesicles. The dynamics of the phase separation of this polymer solution enclosed in vesicles is studied by concentration quench, i.e. exposing the vesicles to hypertonic solutions.