Regeneration of Centella asiaticaplants from non-embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants

Bibi Yamin, Zia Muhammad, Nisa Sobia, Habib Darima, Waheed Abdul, Chaudhary

Description

The threatened plant Centella asiatica L . is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years. Results Present study reveals that 6-benzyladenine (BA) either alone or in combination with 1-naphthalene acetic acid (NAA) supplemented in Murashige and Skoog (MS) medium at different concentrations produced good quality callus from leaf explants of C. asiatica . The calli produced on different plant growth regulators at different concentrations were mostly embryogenic and green. Highest shoot regeneration efficiency; 10 shoots per callus explant, from non-embryogenic callus was observed on 4.42 μM BA with 5.37 μM NAA. Best rooting response was observed at 5.37 and 10.74 μM NAA with 20 average number of roots per explant. Calli and regenerated plants extracts inhibited bacterial growth with mean zone of inhibition 9-13 mm diameter when tested against six bacterial strains using agar well diffusion method. Agar tube dilution method for antifungal assay showed 3.2-76% growth inhibition of Mucor species, Aspergillus fumigatus and Fusarium moliniformes . Conclusions The present investigation reveals that non-embryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium. Furthermore, callus from leaf explant of C. asiatica can be a good source for production of antimicrobial compounds through bioreactor.

Sujets

• Antimicrobial
• Centella asiatica
• Organogénesis
• Plant hormone

Informations

• Publié par: biomed
• Publié le: 01 janvier 2011
• Nombre de lectures: 40
• Langue: English
• Poids de l'ouvrage: 1 Mo

Extrait

Bibiet al.Journal of Biological Engineering2011,5:13 http://www.jbioleng.org/content/5/1/13

R E S E A R C H

Open Access

Regeneration ofCentella asiaticaplants from non embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants 1 2* 1 1 3 1 Yamin Bibi , Muhammad Zia , Sobia Nisa , Darima Habib , Abdul Waheed and Fayyaz M Chaudhary

Abstract Background:The threatened plantCentella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years. Results:Present study reveals that 6benzyladenine (BA) either alone or in combination with 1naphthalene acetic acid (NAA) supplemented in Murashige and Skoog (MS) medium at different concentrations produced good quality callus from leaf explants ofC. asiatica. The calli produced on different plant growth regulators at different concentrations were mostly embryogenic and green. Highest shoot regeneration efficiency; 10 shoots per callus explant, from nonembryogenic callus was observed on 4.42μM BA with 5.37μM NAA. Best rooting response was observed at 5.37 and 10.74μM NAA with 20 average number of roots per explant. Calli and regenerated plants extracts inhibited bacterial growth with mean zone of inhibition 913 mm diameter when tested against six bacterial strains using agar well diffusion method. Agar tube dilution method for antifungal assay showed 3.276% growth inhibition ofMucorspecies,Aspergillus fumigatusandFusarium moliniformes. Conclusions:The present investigation reveals that nonembryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium. Furthermore, callus from leaf explant ofC. asiaticacan be a good source for production of antimicrobial compounds through bioreactor. Keywords:Antimicrobial, Centella asiatica, organogenesis, plant growth regulators

Background In recent years considerable attention has been paid to utilize ecofriendly and biofriendly plant based products, normally secondary metabolites, for the prevention and cure of different human diseases including microbial infections [1]. Moreover, the continuous and nonorga nized exploitation has resulted in many plants becoming rare or extinct. So there is a dire need to set a protocol for in vitro production of plants. In view of commercial importance given to secondary metabolites, efficient pro duction of bioactive compounds by tissue culture tech nology has gained popularity [2]. Secondary metabolites

* Correspondence: [e-mail] 2 Department of Biotechnology, QuaidiAzam University, Islamabad, Pakistan Full list of author information is available at the end of the article

often have a complex stereo structure and many of these cannot be synthesized economically on a commercial basis. To overcome this limitation, biotechnologists sug gest“the use of cell and tissue culture technology rather than the whole plant for the extraction of certain second ary metabolites [3]. Antimicrobial potential of cell culture has been studied in a number of plants i.e.Sesuvium por tulacastrum L, Ephedra strobiliacea, Ephedra procera, Ephedra pachyclada[4,5], and reports are also available for increased metabolite production in callus culture of Calophyllum brasiliense(calanolides), Psoralea drupacea Bge (bakuchiol) andArtemisia absenthium(artemisinin) [68]. Centella asiatica L. commonly known as Indian penny wort belongs to family Apiaceae. Traditionally it is used

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