Responsesof the hyperthermophilic archaeonSulfolobus solfataricusto UV-lightvom Fachbereich Biologieder Technischen Universität Darmstadt zurErlangung des akademischen Grades einesDoctor rerum naturaliumgenehmigteDISSERTATIONVorgelegt vonDipl. Biol. Sabrina Frölsaus Frankfurt am MainDarmstadt 2008D17Tag der Einreichung: 09. April 2008Tag der mündlichen Prüfung: 17. Juni 20081. Berichterstatterin extern : Prof. Dr. Christa Schleper2. Berichterstatterin: Prof. Dr. Felicitas Pfeifer3. Berichterstatter: Dr. habil. Arnulf KletzinCellular aggregation of S. solfataricusafter UV-irradiation.(DAPI stained, fluorescence micrograph)“Nothing in an organism makes sense exceptin the light of the functional context.”(Jan-Hendrik S. Hofmeyr)Index IIndexChapter 1 Summary 1Chapter 2 General Introduction 42.1 Archaea - the third domain of life 42.2 as models for the central information processing in Eukarya 62.3 Sulfolobus solfataricus and its virus SSV1 92.4 solfataricus - an archaeal model system 112.4.1 Cell cycle properties 122.4.2 UV-light induced DNA damage and repair 132.5 Aims of this study 14Chapter 3 Elucidating the transcription cycle of the UV-inducible hyperthermophilic archaeal virus SSV1 by DNA-microarrays3.1 Abstract 163.2 Introduction 173.3 Results 193.3.1 UV-effects on growth of host and virus 193.3.2 Analysis of the transcription cycle 213.3.3 Transcriptional activity in the non-induced state and effects on the host 253.3.