Pestivirus infections of ruminants

European Commission Directorate-General For Agriculture And Rural Development Directorate-General For The Information Society And Media

Description

Agricultural and fisheries research
Veterinary sector and animal health

Sujets

• Cattle
• Infectious disease
• Epidemiology
• Livestock

Informations

• Publié par: EUROPEAN-COMMISSION-DIRECTORATE-GENERAL-FOR-AGRICULTURE-AND-RURAL-DEVELOPMENT-DI
• Nombre de lectures: 37
• Langue: English
• Poids de l'ouvrage: 4 Mo

Extrait

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Commission of the European Communities
AGRICULTURE
Pestivirus infections of ruminants
Report
EUR 10238 EN Commission of the European Communities
AGRICULTURE
Pestivirus infections of ruminants
A seminar in the CEC programme
of coordination of research on
animal husbandry, held in Brussels,
10-11 September 1985
Edited by
J. W. Harkness
Ministry of Agriculture, Fisheries and Food
Central Veterinary Laboratory
New Haw
Weybridge
Surrey
United Kingdom
Sponsored by the
Commission of the European Communities
Directorate-General for Agriculture
Coordination of agricultural research PAnl. VÎT?. BMioth.
N.C./L.. 'I
1987 CL EUR 10238 EN Published by the
COMMISSION OF THE EUROPEAN COMMUNITIES
Directorate-General
Telecommunications, Information Industries and Innovation
Bâtiment Jean Monnet
LUXEMBOURG
LEGAL NOTICE
Neither the Commission of the European Communities nor any person acting on behalf
of then is responsible for the use which might be made of the following
information.
Cataloguing data can be found at the end of this publication.
Luxembourg, Office for Official Publications of the European Communities, 1987
ISBN 92-825-7005-3
Catalogue number: CD-NA-10238-EN-C
© ECSC-EEC-EAEC, Brussels · Luxembourg, 1987
Printed in Belgium PKKKACE
The Pestivirus Genus contains only three viruses, each of which is a
pathogen of significance for farm animals. The most dramatic of the disea­
ses they cause is classical swine fever, but it was with the other condi­
tions. Bovine Viral Diarrhoea (BVD) and Border Disease of Sheep (BD), that
the seminar whose proceedings form the contents of this volume was primar­
ily concerned. It was held on 10-11 September 1985, in Brussels at the
Commission of the European Coranunities (CEC) and was part of the continu­
ing prcxji-ciiiiiie of support for research into Animal Husbandry.
Research on many aspects of pestivirus infections in cattle and sheep
is still at an early stage but the discoveries of the past few years have
exciting and important implications. The papers presented at the seminar
covered a wide range of topics and confirmed that studies at several lab­
oratories have enlarged upon recent advances. The meeting gave an opportu­
nity for scientists from the EEC countries to exchange knowledge and dis­
cuss findings about the viruses themselves and the diseases they cause,
and provided a considerable stimulus to further investigation.
The molecular virology of BVD and BD viruses has been much neglected
until recently. As some of the presentations showed, the application of
new techniques has begun to reveal the secrets of the viral genome and of
the virion's composition. As far as the antigenic characteristics of the
viruses are concerned, there is insufficient data available at present to
permit confident differentiation of the pestiviruses of cattle and sheep,
but with the appearance of monoclonal antibody panels we can expect a much
more precise basis for typing virus strains to emerge. The relationships
between the viruses from cattle and sheep can then be better defined, and
the similarities with and differences from the virus of classical swine
fever can be further explored.
The research effort on ruminant pestiviruses in several laboratories,
both within the countries of the EEC and elsewhere, has brought a clearer
understanding of their biology, and the emergence of new concepts in the
pathogenesis, epidemiology and control of the diseases they 'cause. Not
least has been the realisation that their economic impact in many lives­
tock enterprises is very much greater than was believed hitherto.
Thanks are due to all those who through their contributions helped to
make the seminar a success particularly those who acted as chairmen, and
those who recorded the discussions.
J.W. Harkness
Weybridge, 30 May 1986 CONTENTS
Page
Preface . . . . . . Ill
SESSION I : PROPERTIES OF THE VIRUSES OF BVD AND
BORDER DISEASE
ISOLATION OF CYTOPATHIC AND NON-CYTOPATHIC
BOVINE VIRAL DIARRHOEA VIRUS FROM TISSUES OF
INFECTED ANIMALS
M.C. Clarke, J. Brownlie and C.J. Howard .. 3
DISCUSSION .. .. ..11
CYTOPATHIC AND NON-CYTOPATHIC ISOLATES OF BVDV
IN A CLOSED DAIRY HERD
P.F. Nettleton, A.C. Gardiner, J.S. Gilmour
and D.M.L. Barber .. ..3
DISCUSSION .. . 28
CHARACTERISATION OF VARIOUS STRAINS OF BVD
VIRUS BY MONOCLONAL ANTIBODIES
I. Greiser-Wilke, W. Peters, V. Moennig and
B. Liess .. .. ..29
DISCUSSION.. 42
MOLECULAR VARIATION BETWEEN BVD VIRUS ISOLATES
D.H. Pocock, C.J. Howard, M.C. Clarke and
J. Brownlie .. .. ..43
DISCUSSION. . 52
MOLECULAR CLONING, SEQUENCING AND EXPRESSION
OF BVDV RNA
A. Renard, S. Brown-Shimmer, D. Schmetz,
C. Guiot, L. Dagenais, P.-P. Pastoret, D. Dina
and J. Martial .. .. ..53
DISCUSSION .. . 65
SESSION II : SEROLOGICAL STUDIES AND DIAGNOSTIC METHODS
JIMMUNOENZYME TECHNIQUES FOR BOVINE VIRAL
DIARRHOEA VIRUS
C.J. Howard, J. Brownlie and M.C. Clarke .. 69
DISCUSSION .. .. ..79
AN ELISA FOR BVD VIRUS SEROLOGY
P.J. Straver, W.G.J. Middel, F. Westenbrink
and P.W. de Leeuw .. .. ..81 PRODUCTION OF POTENT ANTISERA RAISED IN PIGS
BY ANAMNESTIC RESPONSE AND USE FOR DIRECT
IMMUNOFLUORESCENT AND IMMUNOPEROXIDASE
TECHNIQUES
J.M.K. Hyera, J. Dahle, B. Liess, V. Moennig
and H.-R. Frey .. .. 87
DISCUSSION ......102
OCCURRENCEOFPESTIVIRUS INFECTIONSINSHEEP
IN FRANCE
P. Russo, A. Giauffret and C. Fleche-Seban .. 103
DISCUSSION .. .. ..110
OBSERVATIONSONNATURALEXPOSURE TOBOVINE
VIRAL DIARRHOEA IN CATTLE
H.M. Gunn .. .. ..m
DISCUSSION....118
SESSION III :PATHOLOGY, PATHOGENESIS AND IMMUNITY
ACCOUNT OF THE ETIOLOGICAL RELATION OF BVD
VIRUS INFECTIONS IN CATTLE TO CLINICALLY
SUSPECT CASES OF BOVINE VIRAL DIARRHOEA
H.-R. Frey, Β. Liess, W. Peters, L. Roming
and B. Lehmann .. .. .. 123
DISCUSSION ......131
INCIDENCEANDIMPACT OF PERSISTENT INFECTIONS
WITH BVDVIRUSIN THE FIELD
W. Peters, B. Liess, H.-R. Frey and G. Trautwein 133
DISCUSSION .. . . · · H4
CLINICALANDEXPERIMENTALMUCOSAL DISEASE-
DEFININGAHYPOTHESIS FORPATHOGENESIS
J. Brownlie, M.C. Clarke and C.J. Howard .. 1^7
DISCUSSION .. .. 157
EXPERIMENTALTRANSPLACENTALTRANSMISSIONOF
BVD VIRUSTOTHE BOVINE FETUS
jB. Liess, S. Orban, H.-R. Frey andG.Trautwein 159
DISCUSSION .. .. 167
CEREBELLARHYPOPLASIA ANDHYDRANENCEPHALYIN
CATTLEASSOCIATED WITH TRANSPLACENTALBVD
VIRUS INFECTION
G. Trautwein, M. Hewicker, Β. Liess, S. Orban
and W. Peters .. .. .. 169
179 DISCUSSION
-VI-SESSION IV : EPIDEMIOLOGY AND CONTROL
METHODS AND STRATEGIES OF BOVINE VIRAL DIARRHOEA
CONTROL IN ITALY
F.M. Cancellotti and F. Carlotto .. .. 183
DISCUSSION .. ..193
INTERSPECIESTRANSMISSIONOFPESTIVIRUS :
EXPERIMENTALINFECTIONS WITH BOVINE VIRAL
DIARRHOEA
195J. Dahle, B. Liess and H.-R. Frey
212
DISCUSSION
INTERHERD CONTROL OF BOVINE VIRAL DIARRHOEA
INFECTIONS IN CATTLE BREEDING HERDS
C. Stahl, Β. Liess, J. Dahle, H.-R. Frey and
E. Grunert 213
DISCUSSION 223
EXPERIMENTAL EXPOSURE OF VACCINATED AND NON-
VACCINATED PREGNANT CATTLE TO ISOLATES OF BOVINE
VIRAL DIARRHOEA VIRUS (BVDV)
A. Meyling, L. R^nsholt, K. Dalsgaard and
Astrid Mikel Jensen .. .. . . 225
232 DISCUSSION
THE EFFICACY OF AN EXPERIMENTAL INACTIVATED
MVD-MD VACCINE
J.W. Harkness, P.L. Roeder, T. Drew, L. Wood and
M. Jeffrey .. .. .. 233
DISCUSSION....250
CONTROL OFBVDV : A CASE FOR SCBA?
C.R.W. Spedding, R.M. Bennett and J.T. Done .. 253
DISCUSSION .. .. 272
GENERALDISCUSSSION ....275
.LIST OF PARTICIPANTS .. .. 278
-VII-