Structural-functional reciprocal affiliations between the phytochrome two-component signal transduction system and intra-domain cross talk in Calothrix PCC 7601 [Elektronische Ressource] / Shivani Sharda

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• Biologie

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• Publié par: heinrich-heine-universitat_dusseldorf
• Publié le: 01 janvier 2010
• Nombre de lectures: 14
• Langue: English
• Poids de l'ouvrage: 6 Mo

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Structural-functional reciprocal affiliations
between the phytochrome two-component
signal transduction system and intra-
domain cross talk in Calothrix PCC 7601
















Shivani Sharda





Structural-functional reciprocal affiliations between the
phytochrome two-component signal transduction system
and intra-domain cross talk in Calothrix PCC 7601

Inaugural Dissertation

zur Erlangung des Doktorgrades
der Mathematisch-Naturwissenschaftlichen Fakultät
der Heinrich- Heine-Universität Düsseldorf


vorgelegt von



Shivani Sharda
Aus Jalandhar (Indien)

Düsseldorf, April 2010
Revisionsschein




Ich bescheinige hiermit, dass mir die Originalfassung der Dissertation
Von Frau ……Shivani Sharda………………………………………………
mit dem Titel …` ‘Structural-functional reciprocal affiliations between the
phytochrome two-component signal transduction system and intra-domain
cross talk in Calothrix PCC 7601’.....……………………............................

vorgelegt worden ist und ich gegen den Druck dieser Dissertation nichts
einzuwenden habe


____________________________
(Ort and Datum)


(Unterschrift Berichterstatter /in und Institütsstempel)


Der unterzeichnet revisionsschein ist zurückzusenden an :






Mathematisch-Naturwissenschaftliche Fakultät
der Heinrich-Heine-Universität Düsseldorf
DER DEKAN
Universitätsstr. 1, 40225 Düsseldorf



ADDENDUM

Acknowledgements
It is a pleasure to thank the many people who made this thesis possible.
This piece of work is an accomplishment of a long journey that has been
blessed by God Almighty, and by people who were ever present behind me for
inspiring and guiding me through thick and thin. The travel through the scientific
corridors of phytochrome research was enlightened with words of encouragement
and intellectual pauses that expanded my knowledge and ideas to greater heights.
The vitally pertinent gratitude is for my Ph.D. supervisor, Professor
Wolfgang Gärtner whose perpetual energy and enthusiasm in research are exalted
by all. For me, he was always accessible and willing to help during my stay in
Germany and otherwise. It is difficult to overstate my sincere thanks to him for his
continuous support during the study, his patience and motivation that guided me
through the research and write up of this thesis. He set benchmarks for getting
solutions to problems that has enthused me to look for brighter prospects in future.
I could not have imagined a better mentor for my Ph.D. study.
I am earnestly grateful to Prof. Georg Büldt at Forschungszentrum, Jülich-
my co-supervisor for giving me the opportunity to work with his group and his
valuable insights on protein crystallography. The work was endearingly supported
with his scrupulous appraisal that was amply constructive for my work.
Solemn gratitude goes to Prof. Georg Groth to agree to be my second referee
for my thesis evaluation and helping me with his worthy inputs. I express my
sincere gratitude to Professor Wolfgang Lubitz for the insightful discussions and
permission to work in his lab.
Special thanks to Professor Silvia Braslavsky and Professor Aba Losi for
their cheery and buoyant selves that put new energy into my work. I would also
ADDENDUM

like to gratefully acknowledge the support and valuable advice of Professor Jon
Hughes to let me have extensive discussions around my work with him.
My regards to Professor Siebert and Pascale Schwinté at University of
Freiburg, who gave me their critical analysis of the proteins I worked upon
facilitating my knowledge into their molecular understanding.
I am indebted to trustworthy collaborations with Professor Martin
Engelhard at MPI Dortmund, Professor Eckhard Hofmann at Ruhr University
Bochum for their extended support during my protein explorations.
Frau Helene Steffen, Tanja Berndsen, Ingeborg Heise and Gül Kóc have been
a constant source of espousal during my project work. I treasure all precious
moments we shared and really like to thank them.
During this work I have collaborated with many colleagues for whom I have
great regard, and I wish to extend my warmest thanks to all those who have
helped me with my work with special mention to Norbert Dickmann, Michael
Reus, Joung-Jun Kim at MPI Dortmund, and Christina Lang at University of
Giessen.
I would like to thank the helpful and caring lab-mates at Max Planck, to
make it a convivial place to work. This extends to all the colleagues who co-
ordinate in good spirits at times of need, and make working more fun during the
long hours in the lab.
My well wishers and friends in the institute gave me their steadfast help
during my difficult times, their detailed review, constructive criticism and
excellent advice has seen me sail through the project work. Amrit, thanks for
tuning in with my frequency personally and working atmosphere in the lab.
Aruna, Julieta, Rashmi, Zhen, Gopal, were fun to work with and grow into a
closely knit protein working group. I thank my fellow lab buddies for stimulating
ADDENDUM

questioning, for their undaunted help and their tireless hours that were spent in
the green aura of the dark room. Christoph, Christian, Anke, Alessandra, Dr. Zakir
Hussain, Dr. Hideaki Ogata, Petra were very supportive in their role as co-workers
and friends.
Daniela and Barbara were two nice girls who worked with me and showed
their skill in learning and adapting to the protein work. There are others who have
assisted me in numerous ways in my work and stay there. Thanks.
I am especially grateful to my friend Dr. Bettina Berendonck for helping me
get through tough times, be it in Germany or during her visit to India, and for all
the emotional support, comraderie, entertainment and care she provided.
The provident staff at MPI had been always welcoming and helpful with
special allusion for Herr Schlamann, Herr Klotzbücher, and Herr Stephan
Kempkes for their timely help. The librarians for providing me documents at short
notices and finding the needed literature for my study. I also thank the
administration staff for their sympathetic help in all paper work.
And to the rest, whom I failed to mention, thank you very much for your
prayers. It really helped a lot. To everybody that has been a part of my life but I
failed to mention, thank you very much.
The words might fall short for my hearty gratitude for my mentors and very
special individuals who showed me the path for a successful career in spite of all
my backtracking experiences. The word document doesn’t sum up my heartfelt
emotions for the endurance showered upon me. They reflected back my ideas of
work and life, so that they echoed in my mind to shape this thesis and future work.
I can only say proper thanks by practically getting high on your expectations
through my future work.
ADDENDUM

To wrap it up, thanks everyone, for making my MPI - Germany life fun,
enjoyable and unforgettable. And thanks for making the “morning at nights”
session untiring and enjoyable experience. Thank you doesn’t seem sufficient but it
is said with appreciation and respect. Thank you all for encouraging me to grow
and expand my thinking and allowing the freedom to pursue my doctoral degree.
Last and stupendously, I am indebted to my parents Promila and Jatinder
Kumar Sharda. They bore me, raised me, trained me, propped and loved me to
reach my dreams. To them I devote this thesis.
I cannot end without my deepest gratitude for my family for their stanch
and unswerving belief and eternal spiritual support system throughout my life. I
wish to thank my entire extended family for providing a loving environment for
me. Their unflinching courage and conviction will always instigate me, and I hope
to continue, in my own ways to refurbish their confidence in me and to them I
dedicate this work.
Sagacious approbation goes to my partner, Shailander, for his endorsement
and time to finish this work. His aura would always be surmised in framing my
work and life. The pristine elite element in my life, Sveysha, angelic daughter born
on Christmas Eve, whose innocent smile puts me in a trance, and melts down my
distress into nowhere.
The generous support from MPI/DFG Foundation is greatly appreciated for
funding my ambition to study abroad in their prestigious institute.
Last but not least, thanks to God, his power bestowed in my life has seen me
through all tests in the past five years. You have made my life more plenteous.
May your name be exalted, honored, and glorified.
Shivani Sharda
MPI Muelheim, 2010
ADDENDUM

Curriculum Vitae
Personal Information
Name Shivani Sharda
Birth date 13.07.1976
Birth place Jalandhar, India
Education
th1994 12 grade schooling (Physics,Chemistry,Biology)