Studies on the role of the Arabidopsis thaliana LATERAL ORGAN BOUNDARY DOMAIN (LBD) gene family [Elektronische Ressource] / Madlen Rast. Gutachter: Rüdiger Simon ; Daniel Schubert

Madlen Ventzislavova

Sujets

• Biologie

Informations

• Publié par: heinrich-heine-universitat_dusseldorf
• Publié le: 01 janvier 2012
• Nombre de lectures: 42
• Langue: Deutsch
• Poids de l'ouvrage: 8 Mo

Extrait

Studies on the role of the Arabidopsis thaliana
LATERAL ORGAN BOUNDARY DOMAIN (LBD)
gene family


Inaugural-Dissertation


zur Erlangung des Doktorgrades
der Mathematisch-Naturwissenschaftlichen Fakultät
der Heinrich-Heine-Universität Düsseldorf


vorgelegt von

Madlen I. Rast
aus Freiberg

Düsseldorf, Juli 2011 aus dem Institut für Genetik
der Heinrich-Heine Universität Düsseldorf











Gedruckt mit der Genehmigung der
Mathematisch-Naturwissenschaftlichen Fakultät der
Heinrich-Heine-Universität Düsseldorf




Referent: Professor Dr. R. Simon
Koreferent: Dr. D. Schubert


Tag der mündlichen Prüfung:
INDEX

1. INTRODUCTION ............................................................................................................ 2
1.1. Embryonic pattern formation .................................................................................... 2
1.2. Organization of the Shoot Apical Meristem (SAM) ................................................... 3
1.2.1. SAM homeostasis and lateral organ formation ......................................................... 4
1.2.2. The meristem-to-organ boundary: more than an extremity of anything .................... 4
(Rast et al., 2008)
1.2.3. The LATERAL ORGAN BOUNDARY DOMAIN GENE (LBD) family .......................13
1.3. Organization of the Root Meristem (RM) .................................................................14
1.3.1. Establishment and maintenance of a functional RM ................................................15
1.4. Auxin perception and signal transduction ................................................................16
1.5. Aims of this study....................................................................................................18
2. MATERIALS AND METHODS ......................................................................................20
2.1. Used materials .........................................20
2.1.1. Chemicals ................................................20
2.1.2. Enzymes ..........................................................................................20
2.1.3. Buffers and Media...................................................................................................20
2.1.4. Antibodies ................................................20
2.1.5. Molecular size standards ........................................20
2.1.6. Membrane and Paper .............................................................................................20
2.1.7. Oligonucleotides .............21
2.1.8. Plasmids ..................................................24
2.1.9. Microorganism .........................................26
2.1.10. Plants .....................................................................................................................26
2.1.11. Software ..................................................28
2.2. Methods ..........................29
2.2.1. Genetic methods ....................................................................................................29
2.2.2. Basic molecular methods ........................................................................................30
2.2.3. Protein interaction studies ........................31
2.2.4. Histological and Cytological techniques ..................................................................32
2.2.5. Microscopy .............................................................................................................33
3. FUNCTIONAL CHARACTERIZATION OF THE LBD GENE FAMILY ..........................35
3.1. Results ...................................................................................................................35
3.1.1. Isolation of LBD gain- and loss-of-function mutants ................................................35
3.1.2. Assigning function to LBD15 ...................................................................................37
3.2. Discussion ..............................................................................................................40
3.2.1. LBD family members regulate specific developmental processes ...........................40
III
INDEX

3.2.2. Conclusions ............................................................................................................42
4. THE ROLE OF JLO IN AUXIN SIGNAL TRANSDUCTION ..........................................44
4.1. Results ...................................................................................................................44
4.1.1. Summary of results published in Bureau et al., 2010 ..............................................44
4.1.2. JAGGED LATERAL ORGANS (JLO) controls auxin dependent patterning during
development of the Arabidopsis embryo (Bureau et al., 2010). ...............................45
4.1.3. Supplemental Data (Bureau et al., 2010) ................................................................59
4.1.4. Outcomes from prior studies ...................................................................................66
4.1.5. JLO expression in postembryonic roots ..................................................................66
4.1.6. JLO response to exogenous auxin treatment ..........................................................66
4.1.7. Genetic interaction between JLO and members of the PLT family ..........................68
4.1.8. Several auxin regulated genes are misexpressed in jlo-2 mutants 69
4.1.9. JLO function is partially mediated by the BDL/MP pathway ....................................71
4.1.10. Genetic interaction between JLO and NPH4 (ARF7) ..............................................72
4.1.11. JLO is required for the expression of TIR1/AFB1 family members ..........................74
4.1.12. Genetic interaction between JLO and TIR1.............................................................74
4.1.13. TIR1 expression is already reduced during jlo-2 embryogenesis ............................76
4.2. Discussion ..............................................................................................................77
4.2.1. JLO regulates auxin dependent gene expression ...................................................77
4.2.2. JLO facilitates auxi BDL degradation ..................................................78
4.2.3. JLO mediates auxin perception by promoting TIR1 expression...............................79
4.2.4. Conclusions ............................................................................................................80
5. JLO AND AS2 ACT TOGETHER TO PROMOTE ORGAN DEVELOPMENT AND
AUXIN TRANSPORT ..............................................................82
5.1. Manuscript Rast et al., 2011 ...................................................................................83
5.1.1. Abstract ..................................................................................................................83
5.1.2. Introduction .............................................................................................................84
5.1.3. Results ....................................................86
5.1.4. Discussion ............................................................................................................100
5.1.5. Experimental Procedures ......................................................................................103
5.1.6. References ...........................................................................................................106
5.1.7. Suplemental Data .................................. 109
6. CONCLUDING DISCUSSION ......................114
6.1. LBD transcription factors contribute to complex regulatory networks .................... 114
6.2. Conclusions ..........................................................................................................116
6.3. Perspectives ..................................................................................116

IV
INDEX

7. SUMMARY ..................................................................................................................119
7.1. ZUSAMMENFASSUNG ........................................................................................ 120
8. LITERATUR .........................................................................................123
9. APPENDIX ............................................................................132
9.1. Abbreviations ...............................................................................132
9.2. List of figures .............................133
9.3. List of tables .........................................................................................................134
9.4. Plasmid maps ................................................................................135
EIDESSTATTLICHE ERKLÄRUNG ....................137
ACKNOWLEDGEMENTS ..................................................................................................138

V









CHAPTER I
INTRODUCTION