Diagnostic Immunohistochemistry E-Book

David J Dabbs

Description

Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin’s lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. This concise and complete resource is today’s indispensable guide to the effective use of immunohistochemical diagnosis.

• Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses.
• Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization.
• Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors.
• Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance.
• Features an expert author for each chapter to ensure coverage of the current state of the art.

Informations

• Publié par: Saunders
• Date de parution: 11 octobre 2013
• Nombre de lectures: 0
• EAN13: 9780323225090
• Langue: English
• Poids de l'ouvrage: 11 Mo

Extrait

Diagnostic Immunohistochemistry
Theranostic and Genomic Applications
Fourth Edition

David J. Dabbs, MD
Professor and Chief of Pathology, Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania
Copyright © 2014 Elsevier
Table of Contents
Cover image
Title page
Copyright
Dedication
Contributors
Foreword
Preface
How to Use this Book
Chapter 1: Techniques of Immunohistochemistry: Principles, Pitfalls, and Standardization
Overview
Basic Principles of Immunohistochemistry
Antibodies as Specific Staining Reagents
Blocking Nonspecific Background Staining
Detection Systems
Quality Control and Standardization
Tissue Fixation, Processing, and Antigen Retrieval Techniques
Techniques, Protocols, and Troubleshooting
Conclusion
Chapter 2: Molecular Anatomic Pathology: P rinciples , T echniques , and A pplication to I mmunohistologic D iagnosis
General Principles of Molecular Biology
Genetic Polymorphism and Mutations
Specimen Requirements for Molecular Testing
Common Techniques for Molecular Analysis
Detection of Small-Scale Mutations
Detection of Chromosomal Rearrangements
Detection of Chromosomal Deletions/Loss of Heterozygosity Analysis
Detection of Microsatellite Instability
DNA-Based Tissue Identity Testing
Summary
Chapter 3: Immunohistology of Infectious Diseases
Overview
Viral Infections
Bacterial Infections
Fungal Infections
Protozoal Infections
Emerging Infectious Diseases
Pathologists, Immunohistochemistry, and Bioterrorism
Beyond Immunohistology: Molecular Diagnostic Applications
Summary
Chapter 4: Immunohistology of Neoplasms of Soft Tissue and Bone
Overview
Biology of Antigens and Antibodies
Specific Soft Tissue and Bone Tumors
Summary
Acknowledgment
Chapter 5: Immunohistology of Hodgkin Lymphoma
Overview
Biology of Antigens and Antibodies
Antibody Specifications
Diagnostic Immunohistochemistry
Molecular Anatomic Pathology
Beyond Immunohistochemistry: Anatomic Molecular Diagnostic Applications
Theranostic Applications
Summary
Chapter 6: Immunohistology of Non-Hodgkin Lymphoma
Overview
Antigens for Evaluation of Hematologic Disorders
Establishing the Diagnosis of Lymphoma: Differential Diagnostic Considerations
Immunohistochemical Evaluation of Small B-Cell Lymphomas
Large B-Cell Lymphomas and Other Aggressive B-Cell Lymphomas
T-Cell Lymphomas
Pitfalls in the Diagnosis of Lymphoma: Mimicry
Summary
Chapter 7: Immunohistology of Melanocytic Neoplasms
Overview
Biology of Antigens and Antibodies
Neuroendocrine Markers in Melanocytic Lesions
Sentinel Lymph Node Biopsies for Metastatic Melanoma
Application of Immunohistochemistry to Selected Differential Diagnoses
Prognostic Markers and Targeted Therapy for Melanoma
Summary
Chapter 8: Immunohistology of Metastatic Carcinoma of Unknown Primary Site
Overview
Cancer of Unknown Primary Site: Clinical Aspects and Economic Considerations
Diagnostic Approach to the Study of CUPS: Specimen Preparation
Determining Site of Origin: Stepwise Approach
Combined Antibody (Panel) Approach to Solving Diagnostic Problems
Special Clinical Presentations
Molecular Approach for Determining Site of Origin
Summary
Chapter 9: Immunohistology of Head and Neck Lesions
Overview
Squamoproliferative Lesions
Nasal Cavity and Paranasal Sinuses
Nasopharynx
Oral Cavity
Larynx/Hypopharynx
Salivary Glands
Ear and Temporal Bone
Metastatic Tumors
Summary
Acknowledgements
Chapter 10: Immunohistology of Endocrine Tumors
Overview
Biology of Antigens and Antibodies
Tumors of Specific Sites
Endocrine Tumors in Other Sites
Summary
Acknowledgements
Chapter 11: Immunohistology of the Mediastinum
Overview
Biology of Antigens and Antibodies
Normal Thymus
Thymic Epithelial Neoplasms
Mediastinal Neuroendocrine Neoplasms
Mesothelial Neoplasms of the Mediastinum
Mesenchymal Neoplasms of the Mediastinum
Mediastinal Germ Cell Tumors
Histiocytic Tumors of the Mediastinum
Lymphoproliferative Disorders of the Mediastinum
Prognostic Markers for Mediastinal Neoplasms
Summary
Chapter 12: Immunohistology of Lung and Pleural Neoplasms
Overview
Primary Lung Neoplasms
Differential Diagnosis and Pitfalls of Lung Neoplasms
Theranostic Applications in Lung Neoplasms
Pleural Neoplasms
Diagnostic Pitfalls of Pleural Neoplasms
Molecular Biology and Theranostic Features in Diffuse Malignant Mesothelioma
Prognosis of Mesothelioma Based on Morphology and Immunohistochemical Analysis
Summary
Chapter 13: Immunohistology of Skin Tumors
Overview
Epithelial Tumors of the Skin
Cutaneous Lymphohematopoietic Disorders
Mesenchymal Tumors of the Skin
Special Topics in Cutaneous Immunohistochemistry
Chapter 14: Immunohistology of the Gastrointestinal Tract
Overview
Biology of Antigens: General and Tissue Specific
Diagnostic Immunohistochemistry
Genomic Applications
Theranostic Applications
Beyond Immunohistochemistry: Anatomic Molecular Diagnostic Applications
Summary
Chapter 15: Immunohistology of Pancreas and Hepatobiliary Tract
Pancreas
Extrahepatic Biliary Tract (Gallbladder and Extrahepatic Bile Ducts)
Ampulla
Liver
Summary
Chapter 16: Immunohistology of the Prostate
Overview
Biology of Antigens and Antibodies
Diagnostic Immunohistochemistry of Specific Prostate Lesions
Beyond Immunohistochemistry: Theranostic and Genomic Applications
Summary
Chapter 17: Immunohistology of the Bladder, Kidney, and Testis
Overview
Immunohistology of the Urinary Bladder
Diagnostic Immunohistochemistry of Specific Bladder Neoplasms
Immunohistology of Renal Neoplasms
Immunohistology of Testicular Tumors
Summary
Chapter 18: Immunohistology of the Female Genital Tract
Overview
Vulva, Vagina, and Cervix
Uterus
Ovary and Fallopian Tubes
Peritoneal Mesothelioma
Summary
Peritoneal Mesothelioma
Summary
Chapter 19: Immunohistology of the Breast
Overview
Myoepithelial Cells and Assessment of Stromal Invasion
Immunohistochemistry of Papillary Lesions
Proliferative Ductal Epithelial Lesions and In Situ Carcinoma
Tumor Type Identification by Immunohistochemistry
Paget Disease of the Breast
Detection of Lymphatic Space Invasion
Sentinel Lymph Node Examination
Systemic Metastasis of Breast Carcinoma
Fibroepithelial Tumors
Theranostic Applications
Genomic Applications of Immunohistochemistry: Breast Cancer Molecular Classification and Immunogenomics
Other Tumor Markers
Summary
Chapter 20: Immunohistology of the Nervous System
Overview
Clinical and Radiologic Perspective of Lesions
Nonneoplastic Brain Lesions
Tumors of the Nervous System
Cysts of the Nervous System
Dementias
Demyelination
Epilepsy
Pitfalls in Diagnosis
Summary
Acknowledgments
Chapter 21: Immunocytology
Overview
Use of Immunocytology
Immunocytology Techniques
Interpretation and Limitations of Immunocytology
Applications of Immunocytology
Theranostic Applications of Immunocytology
Tumors of Unknown Primary
Summary
Chapter 22: Immunohistology of Pediatric Neoplasms
Overview
Biology of Antigens and Antibodies
Specific Tumors
Summary
Acknowledgements
Chapter 23: Imaging and Quantitative Immunohistochemistry
Overview
Imaging Systems
Software Algorithms
Strengths and Limitations
Clinical Applications
Summary
Index
Copyright

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DIAGNOSTIC IMMUNOHISTOCHEMISTRY: THERANOSTIC AND GENOMIC APPLICATIONS ISBN: 978-1-4557-4461-9
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Notices
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