Untersuchungen zur Pathogenität von Autoantikörpern gegen Typ-XVII-Kollagen von Patienten mit Pemphigoid gestationis [Elektronische Ressource] = Pathogenic relevance of autoantibodies to type XVII collagen from pemphigoid gestationis patients / vorgelegt von Josep E. Herrero-González

Josep Carner

Description

Aus der Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie der Universität Würzburg Direktorin: Prof. Dr. med. Eva-Bettina Bröcker Untersuchungen zur Pathogenität von Autoantikörpern gegen Typ XVII Kollagen von Patienten mit Pemphigoid gestationis Pathogenic relevance of autoantibodies to type XVII collagen from pemphigoid gestationis patients Inaugural – Dissertation zur Erlangung der Doktorwürde der Medizinischen Fakultät der Bayerischen Julius-Maximilians-Universität zu Würzburg vorgelegt von Josep E. Herrero-González aus Barcelona, SpanienWürzburg, Dezember 2006 Referent: Prof.Dr.med.DetlefZillikensKoreferent: Prof.Dr.med.EvaBettinaBröckerDekan: Prof.Dr.med.MatthiasFroschTagdermündlichenPrüfung:14.

Sujets

• Gesundheit

Informations

• Publié par: julius-maximilians-universitat_wurzburg
• Publié le: 01 janvier 2007
• Nombre de lectures: 20
• Poids de l'ouvrage: 7 Mo

Extrait

 

                
!" "#$     $%  %  #&  '(& )**     &%    %$ $    (& )** $  &&%  &   * +  
  ,  -$% .  "($% /!-0!       /&
 1!23  "$ 4&       5667  

 

 

Referent: Prof. Dr. med. Detlef Zillikens Koreferent: Prof. Dr. med. EvaBettina Bröcker Dekan: Prof. Dr. med. Matthias Frosch                                     Tag der mündlichen Prüfung: 14. März 2007   Der Promovend ist Arzt

 

  

 

 

*0

   *$            % ( -  -% • atP stnei • Preparation of recombinant and cellderived forms of type XVII collagen • Production of synthetic peptides • Immunofluorescence microscopy, immunoblotting and ELISA • Complement source for immunofluorescence studies and complement inactivation of sera • Affinity purification of total IgG and specific antiBP180 autoantibodies   •Isolation of peripheral blood leucocytes • Treatment of cryostat sections of normal human skin • Assessment of the blisterinducing potential of autoantibodies :           •Preadsorption against the NC16A domain of type XVII collagen does not block autoantibodyinduced dermal epidermal separation in the majority of patients with bullous pemphigoid  

     

 

    

8 7 9

8;

 

 

• Pemphigoid gestationis autoantibodies induce leucocyte dependent dermalepidermal separation in cryosections of human skin • Preadsorption against the NC16A domain of type XVII collagen blocks the split formation induced by autoantibodies from pemphigoid gestationis patients •Binding of autoantibodies to more than one epitope of type XVII collagen results in subepidermal split formation • Pemphigoid gestationis autoantibodies recognize two major epitopes within the NC16A domain of type XVII collagen • Preadsorption against two synthetic peptides including the two major epitopes abolishes dermalepidermal separation induced by pemphigoid gestationis autoantibodies $          <$          4(           >         :$ $,                      

             

    

59 =6 =8 =5 == ;7

 

 

 

  Aa: amino acid BMZ: basal membrane zone BP: bullous pemphigoid BP180: bullous pemphigoid antigen of 180kDa / type XVII collagen BP230: bullous pemphigoid antigen of 230kDa BSA: bovine serum albumin DEJ: dermalepidermal junction ELISA: enzymelinked immunoassay GST: glutathionetransferase HRP: horseradish peroxidase IF: immunofluorescence LAD1: linear IgA disease antigen1 NC16A: 16th noncollagenous A domain PBS: phosphatebuffered saline PG: pemphigoid gestationis SDSPAGE: sodium dodecyl sulfatepolyacrilamide gel electrophoresis TBS: Trisbuffered saline TBST:   Trisbuffered saline plus Tween20